pet21( ) expression vector Search Results


isopropyl β d 1 thiogalactopyranoside iptg inducible vector pet21  (Thermo Fisher)
97
Thermo Fisher isopropyl β d 1 thiogalactopyranoside iptg inducible vector pet21
Isopropyl β D 1 Thiogalactopyranoside Iptg Inducible Vector Pet21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/Isopropyl-beta-D-thiogalactopyranoside+(IPTG)%2C+Dioxane+free%2C+%3E99%25%2C+Ultrapure/pmc08599861-239-5-15
Average 97 stars, based on 1 article reviews
isopropyl β d 1 thiogalactopyranoside iptg inducible vector pet21 - by Bioz Stars, 2026-11
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expression plasmid pet21 orfa  (ATCC)
99
ATCC expression plasmid pet21 orfa
Expression Plasmid Pet21 Orfa, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/Plasmid/us11613728-200-24-50
Average 99 stars, based on 1 article reviews
expression plasmid pet21 orfa - by Bioz Stars, 2026-11
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expression vector pet21 6  (New England Biolabs)
98
New England Biolabs expression vector pet21 6
Strains used in this study
Expression Vector Pet21 6, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/NcoI/pmc05952393-561-17-28
Average 98 stars, based on 1 article reviews
expression vector pet21 6 - by Bioz Stars, 2026-11
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pet21-b(+) expression vectors  (GenScript corporation)
90
GenScript corporation pet21-b(+) expression vectors
Strains used in this study
Pet21 B(+) Expression Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet21b/pmc10940687-220-12-16
Average 90 stars, based on 1 article reviews
pet21-b(+) expression vectors - by Bioz Stars, 2026-11
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pet21 expression vector  (Twist Bioscience)
95
Twist Bioscience pet21 expression vector
Strains used in this study
Pet21 Expression Vector, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/Affinity/pmc11342335-64-21-24
Average 95 stars, based on 1 article reviews
pet21 expression vector - by Bioz Stars, 2026-11
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hindiii  (New England Biolabs)
99
New England Biolabs hindiii
Strains used in this study
Hindiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/HindIII/custom%40r0104%4028955868
Average 99 stars, based on 1 article reviews
hindiii - by Bioz Stars, 2026-11
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xhoi  (New England Biolabs)
99
New England Biolabs xhoi
Strains used in this study
Xhoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/XhoI/custom%40r0146%4028955868
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xhoi - by Bioz Stars, 2026-11
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expression casette 60egp  (GenScript corporation)
90
GenScript corporation expression casette 60egp
Strains used in this study
Expression Casette 60egp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/expression+casette+60egp/pm27485812-38-4-27
Average 90 stars, based on 1 article reviews
expression casette 60egp - by Bioz Stars, 2026-11
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pet15_nesg expression vectors  (GenScript corporation)
90
GenScript corporation pet15_nesg expression vectors
Strains used in this study
Pet15 Nesg Expression Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pet15+nesg++dlrr+b+dlrr+c/pm25580576-398-9-19
Average 90 stars, based on 1 article reviews
pet15_nesg expression vectors - by Bioz Stars, 2026-11
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catalytic domain  (Addgene inc)
94
Addgene inc catalytic domain
Strains used in this study
Catalytic Domain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/pET21+Catalytic+Domain+of+MALT1+(Plasmid+%2348970)/pmc07479270__mmc1-18-1-19
Average 94 stars, based on 1 article reviews
catalytic domain - by Bioz Stars, 2026-11
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site-directed mutagenesis kit  (NZYTech Inc)
90
NZYTech Inc site-directed mutagenesis kit
Strains used in this study
Site Directed Mutagenesis Kit, supplied by NZYTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/mutagenesis+kit/pm36658780-239-1-20
Average 90 stars, based on 1 article reviews
site-directed mutagenesis kit - by Bioz Stars, 2026-11
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bamhi  (Promega)
90
Promega bamhi
Strains used in this study
Bamhi, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet21(+)+expression+vector/bamhi/pm18632128-167-6-7
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Image Search Results


Strains used in this study

Journal: Journal of Bacteriology

Article Title: Signal Peptidase Is Necessary and Sufficient for Site 1 Cleavage of RsiV in Bacillus subtilis in Response to Lysozyme

doi: 10.1128/JB.00663-17

Figure Lengend Snippet: Strains used in this study

Article Snippet: To generate constructs for purification, the target genes were amplified with primers listed and cloned into the expression vector pET21 6′His-rtev digested with the enzymes NcoI and BamHI (New England BioLabs): SipS 35-184 (CDEP3054/CDEP3055; pAC112), full-length SipS (CDEP3385/CDEP3054; pAC110), SipT (CDEP3363/CDEP3364; pCE601), SipU (CDEP3367/CDEP3368; pCE602), and SipV (CDEP3365/CDEP3366; pCE603). table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Primer Sequence, 5′–3′ Description of use CDEP3165 CGGAGCTCGAATTCGGATCCATTTTATCGAATATAACGTTCTCCC pET 6×His Full-length rsiV CDEP3267 TTTAAGAAGGAGATATACATATGCACCATCACCATCACCATATGGATAAGAGATTACAGC pET 6×His Full-length rsiV CDEP3385 TGTATTTTCAGGGCGCCATGATGAAATCAGAAAATGTTTCGAAG pET 6×His Full-length sipS CDEP3054 CTTTGAATTCCGGATCCCATGGAGCCCCAATGGGAAGCTG pET 6×His sipS 38-184 CDEP3386 TGATGGTGACGCTATGTATCCTAC sipS S43A CDEP3387 ACGACATACGGCGCAAAA sipS S43A CDEP3363 TGTATTTTCAGGGCGCCATGGATTTAGTTGAAGGTTCATCTATG pET 6×His Full-length sipT CDEP3364 AGGCCTTTGAATTCCGTTATTTTGTTTGACGCATTTCG pET 6×His Full-length sipT CDEP3365 TGTATTTTCAGGGCGCCATGGTAGAAGGCGTCAGTATG pET 6×His Full-length sipV CDEP3366 AGGCCTTTGAATTCCGTTATTCGGCATCAGAAATCAC pET 6×His Full-length sipV CDEP3367 TGTATTTTCAGGGCGCCATGGTTATTGAAGGATCATCAATGGC pET 6×His Full-length sipU CDEP3368 AGGCCTTTGAATTCCGTTATTTTGCCTGTCTCATCTC pET 6×His Full-length sipU CDEP1561 ATCAACCCGGACGCCGCTCAGTGGATGTCAAAGATCCCT rsiV A66W CDEP1562 AGGGATCTTTGACATCCACTGAGCGGCGTCCGGGTTGAT rsiV A66W CDEP1031 AAAAAACCTCAATTTTACAG Δ sigV :: kan P hs -rsiV + CDEP1012 GAAGGCCATCAAGTCCAGG Δ sigV :: kan P hs -rsiV + CDEP1690 GGATGAATTGTTTTAGTACCTTGTTTTTTCTTCATTGC Δ sigV :: kan P hs -rsiV + CDEP1691 TAAGCTTAATTGTTATCCGCTAAGGAGGCTTTCTTAATGGATAAGAGATTACAG Δ sigV :: kan P hs -rsiV + CDEP1692 GGTACTAAAACAATTCATCC kan P hs template CDEP1693 TAAGCTTAATTGTTATCCGC kan P hs template CDEP1701 CGTCCTGGTTTTGCGCCTGG Δ sipT CDEP1711 CAATTCGCCCTATAGTGAGTCGTAAAGTGACGGATCAGGAGAGCCA Δ sipT CDEP1712 CCAGCTTTTGTTCCCTTTAGTGAGTGCTGGCCTAGGACAGCAGG Δ sipT CDEP1704 GGTGGTTCGTCGGCTCATCGA Δ sipT CDEP1721 GATGTTCACTATGTCAAACATATTATCGGCCTTCCCGGC sipS R84H CDEP1722 GCCGGGAAGGCCGATAATATGTTTGACATAGTGAACATC sipS R84H CDEP3169 TAGAATTCGAGCTCCCGGGAGTAACCTTCATGACGTTAATTG Clone sipS R84H onto pMAD CDEP3170 CATGCCATGGTACCCGGGAATTGCTGTCGGAACATC Clone sipS R84H onto pMAD CDEP1955 ACGACTCACTATAGGGCGAATTGTCCTTACGCGAAATACGGGC CDEP1954 CTCACTAAAGGGAACAAAAGCTGGAGTACAGTCGGCATTATCTC CDEP3053 TGTATTTTCAGGGCGCCATGGTCGTTGATGGTGACTCTATGTATC pET 6×His sipS 38-184 Open in a separate window Oligonucleotides used in this study To clone full-length SipS S43A (CDEP3386/CDEP3387), we conducted PCR amplification with sipS using primer pairs CDEP3054/CDEP3387 and CDEP3388/CDEP3055.

Techniques: