pet-28a backbone Search Results


99
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Addgene inc pet 28a backbone

Pet 28a Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet-28a+backbone/pET-28a(%2B)-FGF2-G3+(Plasmid+%23135521)/pmc08766474-398-23-4
Average 94 stars, based on 1 article reviews
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Pet28a Vector Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
GenScript corporation nadv genes

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Addgene inc backbone plasmid pet28a sumo nup53xl 1 320

Backbone Plasmid Pet28a Sumo Nup53xl 1 320, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Twist Bioscience pet 28a backbone

Pet 28a Backbone, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: eLife

Article Title: In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates

doi: 10.7554/eLife.64252

Figure Lengend Snippet:

Article Snippet: The 6xHis-MBP tag was PCR-amplified from pDZ2087 construct (Addgene, #92414) with primers MBP F and MBP R. Full-length 6xHis-MBP was cloned into pET28a backbone with Xba I and Eco RI restriction sites. mGFP-FUS-TIS-Strep-tag II was PCR-amplified from pcDNA-mGFP-FUS-TIS construct with primers mGFP F and TIS RBD-Strep-tag R. The Strep-tag II sequence was incorporated into primer TIS RBD-Strep-tag R. Full-length mGFP-FUS-TIS-Strep-tag II was cloned into pET28a-6xHis-MBP backbone with Nhe I and Eco RI restriction sites.

Techniques: Transfection, Construct, Recombinant, Expressing, Plasmid Preparation, In Vitro, Sequencing, Purification, Mutagenesis, Bradford Protein Assay, Bradford Assay, Protein Quantitation, Software, Strep-tag, Imaging