Journal: Cell reports

Article Title: Clonal expansion and diversification of germinal center and memory B cell responses to booster immunization in primates

doi: 10.1016/j.celrep.2025.116142

Figure Lengend Snippet: (A) Circos plots showing B cell clone sharing between Wu-1 RBD bait-binding memory and GC B cells in rhesus macaques following three mRNA SARS-CoV-2 immunizations. Segment size is proportional to the clone size, with the small light gray-colored segments denoting singlets and the darker gray indicating expanded clones. The magenta wedge denotes splenic memory clones, teal denotes GC B cell clones identified in the LHS auxiliary lymph nodes (aLNs), and yellow denotes GC B cell clones identified in the RHS aLNs. Conjoined cross-compartment segments indicate clones present in multiple compartments; gray = singlet-singlet, green = expanded clone-singlet, and purple = expanded clone-expanded clone. (B) Germline nucleotide mismatch in GC B cells from the boosted side from each site condition. (C and D) Graphs of the (C) nucleotide mismatch in both sides in contralaterally boosted macaques and (D) SHM of clones belonging to families present in both the LHS and RHS GCs. (E) Phylogenetic trees of individual B cell clonal families based on Ig heavy and light sequences rooted to their inferred germline precursor (iGL). Nodes comprise a single cell unless otherwise marked. Node color denotes the location and fate of the originating cell. (F) Plots showing the germline nucleotide mismatch of heavy-chain and light-chain sequences of memory and GC B cells. (G) Plot showing the paired germline nucleotide mismatch of clones with family members present in both the GC and memory compartment. In cases where more than one cell is in a compartment, the median was used. Data were compared using a paired Student’s t test. (H) Plot showing the germline nucleotide mismatch of cells belonging to clonal families shared across multiple compartments or only present in a single compartment. (B–D, G, and H) Data were compared using a Student’s t test. Each point is the mutation load (cumulative germline mismatch of heavy and light chains) for a single cell. Data are pooled from all animals.

Article Snippet: Anti-human goat IgG (H + L) HRP , Jackson ImmunoResearch , 109–035-088; RRID: AB_2337584.

Techniques: Binding Assay, Clone Assay, Mutagenesis