Journal: bioRxiv

Article Title: The 9p21.3 Coronary Artery Disease Risk Locus Modulates Vascular Cell-State Transitions via Enhancer-Driven Regulation of MTAP

doi: 10.1101/2025.11.18.689066

Figure Lengend Snippet: Characterization of 9p21.3 enhancers and identification of cell-types mediating risk for CAD (A-D) Plots showing super enhancers (blue), enhancers (red), and enhancers within 9p21.3 haplotype (green). (E-G) Dot plots showing enrichment for CAD heritability at baseline and in response to CAD-relevant stimulatory conditions, including Inflammatory (IL-1α and IL-6 & TNF-α) atrial derived fibroblast, coronary artery derived endothelial cells, and adipose derived pericytes. Solid dark lines around each dot represent significance at a threshold of -log 10 (adjusted P-value) ≥ 3.35. (H-I) Dot plots showing the impact of CAD-relevant stimulatory conditions on enhancer activities (proxy of H3K27ac) at 9p21.3 locus in endothelial cells and pericytes. Fold changes are indicated as Red (increased fold change) or Blue (Reduced fold change) and solid dark lines around each dot represent significance at a threshold of -log 10 (FDR) ≥ 1.3. (J) T-statistic versus absolute t-statistic (y-axis) for gene expression across five primary human vascular cell types under basal conditions. Differentially expressed genes, representing the top 10% by absolute t-statistic, are highlighted in red; all other genes are in blue. The dashed line indicates a high absolute t-statistic threshold.

Article Snippet: We utilized primary human cells, including atrial fibroblast (Lonza, CC-2903), ventricular fibroblast (Lonza, CC-2904), coronary artery endothelial cells (Lonza, CC-2585), pulmonary artery smooth muscle cells (Lonza, CC-2581), coronary artery smooth muscle cells (Lonza, CC-2583), and adipose derived microvascular pericytes (Angioproteomie, cAP-0043).

Techniques: Derivative Assay, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

doi: 10.3390/ijms22094604

Figure Lengend Snippet: Representative photomicrographs of human adipose mesenchymal stem cells cultured in basal medium (ASC) or in pericyte medium (pmASC), stained by crystal violet during cellular proliferation assays. After three days of growth in their respective medium, glucose was added in some samples (High Glucose, 25 mM, HG), whereas other samples were kept in normal glucose (NG) condition. Pictures were taken after 72 h from glucose addition and compared to NG samples. A : ASC NG; B : pmASC NG; C : ASC HG; D : pmASC HG. In each image, two magnification levels are shown (10× and 4×, respectively). Scale bar: 100 µm.

Article Snippet: HRPCs were seeded in poly-L-lysine (0.01 mg/mL solution, Innoprot) coated culture flasks and incubated at 37 °C with 5% CO 2 in a specific pericyte medium (PM), containing 2% fetal bovine serum (FBS), 1% pericyte growth supplement (PGS), and 1% penicillin/streptomycin (Innoprot).

Techniques: Cell Culture, Staining

Journal: International Journal of Molecular Sciences

Article Title: Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

doi: 10.3390/ijms22094604

Figure Lengend Snippet: High glucose effects on cell proliferation and viability in human adipose mesenchymal stem cells cultured in basal medium (ASC) or in pericyte medium (pmASC), and in human retinal pericytes (HRPC). In some samples, glucose was added to the culture medium (High Glucose, 25 mM, HG); data were gathered after 24 h, 48 h and 72 h from glucose addition and compared to corresponding samples kept in normal glucose (NG) condition. Proliferation rate was assessed by crystal violet assays in ASC, pmASC ( A ), and HRPC cultures ( B ) in NG or in HG. Cell viability was evaluated by MTT assays in ASC, pmASC ( C ), and HRPC cultures ( D ) in NG or in HG. Absorbance values were determined at 570 nm for both assays. Values are expressed as mean ± SEM of three independent experiments. In A and C, values are referred to ASC NG population, at each corresponding time point. In B and D, values are referred to HRPC NG population, at each corresponding time point. * p < 0.05 pmASC vs. ASC; # p < 0.05 HG vs. NG. Two-way ANOVA, followed by Sidak’s test.

Article Snippet: HRPCs were seeded in poly-L-lysine (0.01 mg/mL solution, Innoprot) coated culture flasks and incubated at 37 °C with 5% CO 2 in a specific pericyte medium (PM), containing 2% fetal bovine serum (FBS), 1% pericyte growth supplement (PGS), and 1% penicillin/streptomycin (Innoprot).

Techniques: Cell Culture

Journal: International Journal of Molecular Sciences

Article Title: Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

doi: 10.3390/ijms22094604

Figure Lengend Snippet: Cell migration ability evaluated by wound-healing assays in human adipose mesenchymal stem cells cultured in basal medium (ASC) or in pericyte medium (pmASC), and in cultures of human retinal pericytes (HRPC). In some samples of each culture type, glucose was added to the culture medium (High Glucose, 25 mM, HG) whereas other samples were kept in normal glucose (NG) condition. ( A ): Representative pictures of each sample taken immediately after the scratch (0 h), after 24 h and 48 h of culture. Scale bar: 100µm (Magnification: 4×). Percentage of wound closure was quantified by Image J software for ASC and pm ASC cultures ( B ), and HRPC cultures ( C ). Values are expressed as mean ± SEM of three independent experiments. * p < 0.05 pmASC vs. ASC; # p < 0.05 HG vs. NG. Two-way ANOVA, followed by Sidak’s test.

Article Snippet: HRPCs were seeded in poly-L-lysine (0.01 mg/mL solution, Innoprot) coated culture flasks and incubated at 37 °C with 5% CO 2 in a specific pericyte medium (PM), containing 2% fetal bovine serum (FBS), 1% pericyte growth supplement (PGS), and 1% penicillin/streptomycin (Innoprot).

Techniques: Migration, Cell Culture, Software

Journal: International Journal of Molecular Sciences

Article Title: Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

doi: 10.3390/ijms22094604

Figure Lengend Snippet: High glucose effects on ROS levels evaluated by H2DCFDA assays in ( A ) human adipose mesenchymal stem cells cultured in basal medium (ASC) or in pericyte medium (pmASC), and in ( B ) human retinal pericytes (HRPC). In some samples, glucose was added to the culture medium (High Glucose, 25 mM, HG); data were gathered after 24 h, 48 h and 72 h from glucose addition, and compared to samples kept in normal glucose (NG) condition. ROS values of each group are normalized by the corresponding population density, as determined by crystal violet staining, at each corresponding time point (ROS/CV ratio). Values are expressed as mean ± SEM of three independent experiments. * p < 0.05 pmASC vs. ASC; # p < 0.05 HG vs. NG; ‡ p < 0.05 72 h vs. 24 h; Two-way ANOVA, followed by Sidak’s test.

Article Snippet: HRPCs were seeded in poly-L-lysine (0.01 mg/mL solution, Innoprot) coated culture flasks and incubated at 37 °C with 5% CO 2 in a specific pericyte medium (PM), containing 2% fetal bovine serum (FBS), 1% pericyte growth supplement (PGS), and 1% penicillin/streptomycin (Innoprot).

Techniques: Cell Culture, Staining

Journal: International Journal of Molecular Sciences

Article Title: Effects of High Glucose Concentration on Pericyte-Like Differentiated Human Adipose-Derived Mesenchymal Stem Cells

doi: 10.3390/ijms22094604

Figure Lengend Snippet: High glucose effects on anti- and pro-inflammatory cytokines and angiogenic factors in human adipose mesenchymal stem cells cultured in basal medium (ASC) or in pericyte medium (pmASC). In some samples, glucose was added to the culture medium (High Glucose, 25 mM, HG); data were gathered after 72 h from glucose addition and compared to corresponding samples kept in normal glucose (NG) condition. Quantitative analysis of IL 10 ( A ), TGF-β1 ( B ), TNFα ( C ), IL-1β ( D ), VEGF ( E ), Angiopoietin-2 ( F ) and MMP9 ( G ) mRNA levels, as evaluated by qRT-PCR. mRNA levels of each group were normalized to the housekeeping reference gene ribosomal 18S RNA. In the histograms, values are expressed as a fold change of those detected in ASCs cultured in NG condition. Each value represents mean ± SEM obtained from three independent experiments. * p < 0.05 pmASC vs. ASC; # p < 0.05 HG vs. NG. Two-way ANOVA, followed by Sidak’s test.

Article Snippet: HRPCs were seeded in poly-L-lysine (0.01 mg/mL solution, Innoprot) coated culture flasks and incubated at 37 °C with 5% CO 2 in a specific pericyte medium (PM), containing 2% fetal bovine serum (FBS), 1% pericyte growth supplement (PGS), and 1% penicillin/streptomycin (Innoprot).

Techniques: Cell Culture, Quantitative RT-PCR