Journal: PLoS ONE

Article Title: Human Peripheral Blood Mononuclear Cell Function and Dendritic Cell Differentiation Are Affected by Bisphenol-A Exposure

doi: 10.1371/journal.pone.0161122

Figure Lengend Snippet: Dendritic Cells (mDCs) (n = 6) were differentiated from PBMCs for 6 days in absence or in presence of BPA at 1nM concentration and then analyzed by flow cytometer. (A) Percentages on total mDCs of cells expressing CD11c, CD86 and HLA-DR, (B) Median of fluorescence intensity of CD11c, CD86 and HLA-DR on mDCs. (C) Examples of staining for CD11c and CD1a on mDCs differentiated with or without BPA. (D) Percentages on total mDCs of cells expressing CD1a. (E) Mixed Lymphocyte Reactions (MLR) (n = 5) were performed with mDCs differentiated and subsequently LPS-matured in presence or absence of BPA, and co-incubated with allogenic non-adherent fractions of PBMCs (1:5 ratio mDCs:NA-cells). Experiments were performed in triplicates and IFN-γ secretion was evaluated after 48 hours. In panels A, B, D and E we reported median and ranges [min max] of the analyzed samples. Asterisks indicate statistically significant differences (*p<0.05) by non-parametric Friedman test, followed by the Wilcoxon test for dependent samples, except for MLR experiments, where paired T-test was applied. In panels A, B, D and E we reported median and ranges [min max] of the analyzed samples. Asterisks indicate statistically significant differences (*p<0.05) by non-parametric Friedman test, followed by the Wilcoxon test for dependent samples.

Article Snippet: Conjugated antibodies for FACS analysis were purchased as follow: PerCP-anti-CD3, PerCP-anti-HLA-DR and PE-anti-CD11c from Miltenyi Biotech, FITC-anti-CD14 from eBioscience (San Diego, CA), PE-anti-CD86 and FITC-anti-CD1a from BD Pharmingen (San Diego, CA, USA).

Techniques: Concentration Assay, Flow Cytometry, Expressing, Fluorescence, Staining, Incubation