Journal: British Journal of Cancer

Article Title: Targeting ErbB3-mediated stromal–epithelial interactions in pancreatic ductal adenocarcinoma

doi: 10.1038/bjc.2011.263

Figure Lengend Snippet: IHC analysis of xenograft tumours with and without CAF and effect of erlotinib treatment. The photographs ( A , B ) show low power (4 × ) representative sections of α -SMA staining of AsPC-1 and AsPC-1+CAF xenografts, respectively. The photographs ( C , D ) show representative sections of pErbB3 expression in erlotinib-treated AsPC-1 and AsPC-1+CAF xenografts. A high power (20 × ) view demonstrates positive membranous pattern, scored as intensity 2 on a 0–3 scale ( D ), the opposite ( C ) shows negative staining. The photographs ( E , F ) show representative sections of pErbB3 staining in placebo-treated AsPC-1 and AsPC-1+CAF xenografts.

Article Snippet: Sections were then incubated overnight with primary monoclonal antibodies for pErbB3 and α -SMA (Santa Cruz Biotechnology).

Techniques: Staining, Expressing, Negative Staining

Journal: BMC Neuroscience

Article Title: MMP-28 as a regulator of myelination

doi: 10.1186/1471-2202-9-83

Figure Lengend Snippet: Signal pathway changes in MMP-28 treated DRG cultures . A) Western blot analysis of pErbB-2, pErbB3, pPI3K (p55/p85) and phosphoMAPK in rat DRG co-cultures at 0, 1, 5, 10, 15, and 20 minutes following addition of fresh media with or without 10 nM MMP-28. Equal protein loading was determined by Ponceau-S staining of the membrane (not shown) and Coomassie staining of a parallel, equally loaded gel. B) Immunofluorescence of pErbB3 in DRG co-cultures 20 minutes after addition of fresh media containing 10 nM MMP-28 preincubated with 30 nM rabbit IgG, pAb180 or pAb183. Blue:DAPI, Red:pErbB3, Green:Acetylated C,D) Determination of proliferation in DRG co-cultures following MMP-28 treatment using PCNA. C) Relative fluorescence units were counted in 14 day myelinating cultures in control and treated samples. D) Percent of proliferating cells after 2 days in culture was determined by counting PCNA positive nuclei. N = 3, ± SD.

Article Snippet: Antibodies were obtained from Cell Signaling Technologies, catalogue numbers as follows: pErbB2 2249S, pErbB3 4784P, pMAPK 4377S, pPI3K 4228P, or LabVision: PCNA Ab-1, PC10.

Techniques: Western Blot, Staining, Immunofluorescence, Fluorescence