pentr vector Search Results


pentr 1a dual selection vector  (Thermo Fisher)
N/A
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pentr™/u6 rnai entry vector  (Promega)
90
Promega pentr™/u6 rnai entry vector
Pentr™/U6 Rnai Entry Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+vector/pentr++u6+rnai+entry+vector/pm31816429-53-6-22
Average 90 stars, based on 1 article reviews
pentr™/u6 rnai entry vector - by Bioz Stars, 2026-09
90/100 stars
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pentr-topo-gas6 vector  (BioResource International Inc)
90
BioResource International Inc pentr-topo-gas6 vector
Effect of C -mannosylation of AXL in MDA-MB-231 cells. (A) Establishment of AXL knockout (AXL KO), re-expressing wild-type AXL (wt), and W320F mutant (WF) MDA-MB-231 cells. Parental (Pt), AXL KO, wt, and WF MDA-MB-231 cells were lysed, and each cell lysate was electrophoresed and immunoblotted with anti-AXL antibody. (B) Establishment of <t>GAS6-overexpressing</t> CHO-K1 cells. CHO-K1 cells transfected with pCI-neo empty vector (CHO-K1-neo) and GAS6-overexpressing CHO-K1 cells (CHO-K1-GAS6) were cultured in serum-free medium for 24 h. The cell lysates and conditioned media were electrophoresed and immunoblotted with anti-c-myc antibody. (C) Effect of C -mannosylation of AXL on its downstream signaling under GAS6 stimulation. Parental, AXL KO, wt, and WF MDA-MB-231 cells were cultured with serum-free DMEM for 24 h. These cells were treated with conditioned medium of CHO-K1-neo (GAS6 -) or CHO-K1-GAS6 (GAS6 +) for 15 min. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies. (D) VM formation assay of parental, AXL KO, wt, and WF MDA-MB-231 cells. These cells were seeded on Matrigel-coated well plates. Photographs were taken and the quantification of VM formation was performed at 5 h after seeding. Scale bar, 200 μm. Data shown are means ± SD. * P < 0.05. (E and F) Evaluation of phosphorylation levels of ERK (E) and AKT (F) of parental, AXL KO, wt, and WF MDA-MB-231 cells under normal culture conditions. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies.
Pentr Topo Gas6 Vector, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+vector/pentr+topo+gas6+vector/pmc11632359-167-7-10
Average 90 stars, based on 1 article reviews
pentr-topo-gas6 vector - by Bioz Stars, 2026-09
90/100 stars
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pentr-d/topo cloning vector  (GenScript corporation)
90
GenScript corporation pentr-d/topo cloning vector
Effect of C -mannosylation of AXL in MDA-MB-231 cells. (A) Establishment of AXL knockout (AXL KO), re-expressing wild-type AXL (wt), and W320F mutant (WF) MDA-MB-231 cells. Parental (Pt), AXL KO, wt, and WF MDA-MB-231 cells were lysed, and each cell lysate was electrophoresed and immunoblotted with anti-AXL antibody. (B) Establishment of <t>GAS6-overexpressing</t> CHO-K1 cells. CHO-K1 cells transfected with pCI-neo empty vector (CHO-K1-neo) and GAS6-overexpressing CHO-K1 cells (CHO-K1-GAS6) were cultured in serum-free medium for 24 h. The cell lysates and conditioned media were electrophoresed and immunoblotted with anti-c-myc antibody. (C) Effect of C -mannosylation of AXL on its downstream signaling under GAS6 stimulation. Parental, AXL KO, wt, and WF MDA-MB-231 cells were cultured with serum-free DMEM for 24 h. These cells were treated with conditioned medium of CHO-K1-neo (GAS6 -) or CHO-K1-GAS6 (GAS6 +) for 15 min. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies. (D) VM formation assay of parental, AXL KO, wt, and WF MDA-MB-231 cells. These cells were seeded on Matrigel-coated well plates. Photographs were taken and the quantification of VM formation was performed at 5 h after seeding. Scale bar, 200 μm. Data shown are means ± SD. * P < 0.05. (E and F) Evaluation of phosphorylation levels of ERK (E) and AKT (F) of parental, AXL KO, wt, and WF MDA-MB-231 cells under normal culture conditions. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies.
Pentr D/Topo Cloning Vector, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+vector/pentr+d+topo+cloning+vector/pmc05872146-159-16-20
Average 90 stars, based on 1 article reviews
pentr-d/topo cloning vector - by Bioz Stars, 2026-09
90/100 stars
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pentr vector  (Promega)
90
Promega pentr vector
Effect of C -mannosylation of AXL in MDA-MB-231 cells. (A) Establishment of AXL knockout (AXL KO), re-expressing wild-type AXL (wt), and W320F mutant (WF) MDA-MB-231 cells. Parental (Pt), AXL KO, wt, and WF MDA-MB-231 cells were lysed, and each cell lysate was electrophoresed and immunoblotted with anti-AXL antibody. (B) Establishment of <t>GAS6-overexpressing</t> CHO-K1 cells. CHO-K1 cells transfected with pCI-neo empty vector (CHO-K1-neo) and GAS6-overexpressing CHO-K1 cells (CHO-K1-GAS6) were cultured in serum-free medium for 24 h. The cell lysates and conditioned media were electrophoresed and immunoblotted with anti-c-myc antibody. (C) Effect of C -mannosylation of AXL on its downstream signaling under GAS6 stimulation. Parental, AXL KO, wt, and WF MDA-MB-231 cells were cultured with serum-free DMEM for 24 h. These cells were treated with conditioned medium of CHO-K1-neo (GAS6 -) or CHO-K1-GAS6 (GAS6 +) for 15 min. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies. (D) VM formation assay of parental, AXL KO, wt, and WF MDA-MB-231 cells. These cells were seeded on Matrigel-coated well plates. Photographs were taken and the quantification of VM formation was performed at 5 h after seeding. Scale bar, 200 μm. Data shown are means ± SD. * P < 0.05. (E and F) Evaluation of phosphorylation levels of ERK (E) and AKT (F) of parental, AXL KO, wt, and WF MDA-MB-231 cells under normal culture conditions. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies.
Pentr Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+vector/pentr+vector/pmc07140906-279-58-63
Average 90 stars, based on 1 article reviews
pentr vector - by Bioz Stars, 2026-09
90/100 stars
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pentr/u6/shrna/gfp vector  (BGI Shenzhen)
90
BGI Shenzhen pentr/u6/shrna/gfp vector
Effect of C -mannosylation of AXL in MDA-MB-231 cells. (A) Establishment of AXL knockout (AXL KO), re-expressing wild-type AXL (wt), and W320F mutant (WF) MDA-MB-231 cells. Parental (Pt), AXL KO, wt, and WF MDA-MB-231 cells were lysed, and each cell lysate was electrophoresed and immunoblotted with anti-AXL antibody. (B) Establishment of <t>GAS6-overexpressing</t> CHO-K1 cells. CHO-K1 cells transfected with pCI-neo empty vector (CHO-K1-neo) and GAS6-overexpressing CHO-K1 cells (CHO-K1-GAS6) were cultured in serum-free medium for 24 h. The cell lysates and conditioned media were electrophoresed and immunoblotted with anti-c-myc antibody. (C) Effect of C -mannosylation of AXL on its downstream signaling under GAS6 stimulation. Parental, AXL KO, wt, and WF MDA-MB-231 cells were cultured with serum-free DMEM for 24 h. These cells were treated with conditioned medium of CHO-K1-neo (GAS6 -) or CHO-K1-GAS6 (GAS6 +) for 15 min. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies. (D) VM formation assay of parental, AXL KO, wt, and WF MDA-MB-231 cells. These cells were seeded on Matrigel-coated well plates. Photographs were taken and the quantification of VM formation was performed at 5 h after seeding. Scale bar, 200 μm. Data shown are means ± SD. * P < 0.05. (E and F) Evaluation of phosphorylation levels of ERK (E) and AKT (F) of parental, AXL KO, wt, and WF MDA-MB-231 cells under normal culture conditions. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies.
Pentr/U6/Shrna/Gfp Vector, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+vector/pentr+u6+shrna+gfp+vector/pmc05376318-95-14-16
Average 90 stars, based on 1 article reviews
pentr/u6/shrna/gfp vector - by Bioz Stars, 2026-09
90/100 stars
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entry vectors (pentr)  (BioResource International Inc)
90
BioResource International Inc entry vectors (pentr)
Effect of C -mannosylation of AXL in MDA-MB-231 cells. (A) Establishment of AXL knockout (AXL KO), re-expressing wild-type AXL (wt), and W320F mutant (WF) MDA-MB-231 cells. Parental (Pt), AXL KO, wt, and WF MDA-MB-231 cells were lysed, and each cell lysate was electrophoresed and immunoblotted with anti-AXL antibody. (B) Establishment of <t>GAS6-overexpressing</t> CHO-K1 cells. CHO-K1 cells transfected with pCI-neo empty vector (CHO-K1-neo) and GAS6-overexpressing CHO-K1 cells (CHO-K1-GAS6) were cultured in serum-free medium for 24 h. The cell lysates and conditioned media were electrophoresed and immunoblotted with anti-c-myc antibody. (C) Effect of C -mannosylation of AXL on its downstream signaling under GAS6 stimulation. Parental, AXL KO, wt, and WF MDA-MB-231 cells were cultured with serum-free DMEM for 24 h. These cells were treated with conditioned medium of CHO-K1-neo (GAS6 -) or CHO-K1-GAS6 (GAS6 +) for 15 min. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies. (D) VM formation assay of parental, AXL KO, wt, and WF MDA-MB-231 cells. These cells were seeded on Matrigel-coated well plates. Photographs were taken and the quantification of VM formation was performed at 5 h after seeding. Scale bar, 200 μm. Data shown are means ± SD. * P < 0.05. (E and F) Evaluation of phosphorylation levels of ERK (E) and AKT (F) of parental, AXL KO, wt, and WF MDA-MB-231 cells under normal culture conditions. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies.
Entry Vectors (Pentr), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pentr+vector/entry+vectors++pentr+/pm24593985-60-5-17
Average 90 stars, based on 1 article reviews
entry vectors (pentr) - by Bioz Stars, 2026-09
90/100 stars
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pentr 4 dual selection vector  (Thermo Fisher)
N/A
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gateway pentr 11 vector  (Thermo Fisher)
N/A
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Image Search Results


Effect of C -mannosylation of AXL in MDA-MB-231 cells. (A) Establishment of AXL knockout (AXL KO), re-expressing wild-type AXL (wt), and W320F mutant (WF) MDA-MB-231 cells. Parental (Pt), AXL KO, wt, and WF MDA-MB-231 cells were lysed, and each cell lysate was electrophoresed and immunoblotted with anti-AXL antibody. (B) Establishment of GAS6-overexpressing CHO-K1 cells. CHO-K1 cells transfected with pCI-neo empty vector (CHO-K1-neo) and GAS6-overexpressing CHO-K1 cells (CHO-K1-GAS6) were cultured in serum-free medium for 24 h. The cell lysates and conditioned media were electrophoresed and immunoblotted with anti-c-myc antibody. (C) Effect of C -mannosylation of AXL on its downstream signaling under GAS6 stimulation. Parental, AXL KO, wt, and WF MDA-MB-231 cells were cultured with serum-free DMEM for 24 h. These cells were treated with conditioned medium of CHO-K1-neo (GAS6 -) or CHO-K1-GAS6 (GAS6 +) for 15 min. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies. (D) VM formation assay of parental, AXL KO, wt, and WF MDA-MB-231 cells. These cells were seeded on Matrigel-coated well plates. Photographs were taken and the quantification of VM formation was performed at 5 h after seeding. Scale bar, 200 μm. Data shown are means ± SD. * P < 0.05. (E and F) Evaluation of phosphorylation levels of ERK (E) and AKT (F) of parental, AXL KO, wt, and WF MDA-MB-231 cells under normal culture conditions. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies.

Journal: Glycobiology

Article Title: Identification of C -mannosylation in a receptor tyrosine kinase AXL

doi: 10.1093/glycob/cwae096

Figure Lengend Snippet: Effect of C -mannosylation of AXL in MDA-MB-231 cells. (A) Establishment of AXL knockout (AXL KO), re-expressing wild-type AXL (wt), and W320F mutant (WF) MDA-MB-231 cells. Parental (Pt), AXL KO, wt, and WF MDA-MB-231 cells were lysed, and each cell lysate was electrophoresed and immunoblotted with anti-AXL antibody. (B) Establishment of GAS6-overexpressing CHO-K1 cells. CHO-K1 cells transfected with pCI-neo empty vector (CHO-K1-neo) and GAS6-overexpressing CHO-K1 cells (CHO-K1-GAS6) were cultured in serum-free medium for 24 h. The cell lysates and conditioned media were electrophoresed and immunoblotted with anti-c-myc antibody. (C) Effect of C -mannosylation of AXL on its downstream signaling under GAS6 stimulation. Parental, AXL KO, wt, and WF MDA-MB-231 cells were cultured with serum-free DMEM for 24 h. These cells were treated with conditioned medium of CHO-K1-neo (GAS6 -) or CHO-K1-GAS6 (GAS6 +) for 15 min. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies. (D) VM formation assay of parental, AXL KO, wt, and WF MDA-MB-231 cells. These cells were seeded on Matrigel-coated well plates. Photographs were taken and the quantification of VM formation was performed at 5 h after seeding. Scale bar, 200 μm. Data shown are means ± SD. * P < 0.05. (E and F) Evaluation of phosphorylation levels of ERK (E) and AKT (F) of parental, AXL KO, wt, and WF MDA-MB-231 cells under normal culture conditions. Each cell lysate was electrophoresed and immunoblotted with anti-ERK2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-AKT, and anti-phospho-AKT (Ser473) antibodies.

Article Snippet: Human GAS6 gene was amplified from the pENTR-TOPO-GAS6 vector (RIKEN BioResource Center) using the primers as follows: 5’-TTTTGAATTCATGGCCCCTTCGCTCTCGCC-3’ (forward), 5’- GATGAGTTTTTGTTCGGCTGCGGCGGGCTC-3’ (reverse).

Techniques: Knock-Out, Expressing, Mutagenesis, Transfection, Plasmid Preparation, Cell Culture, Tube Formation Assay