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Image Search Results
Journal: Scientific reports
Article Title: Loss of Neogenin1 in human colorectal carcinoma cells causes a partial EMT and wound-healing response.
doi: 10.1038/s41598-019-40886-y
Figure Lengend Snippet: Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein EB1. (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
Article Snippet: C-terminal GFP-tagged
Techniques: Knockdown, Binding Assay, Immunostaining, Western Blot, Control