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Image Search Results
Journal:
Article Title: Pituitary adenylate cyclase-activating polypeptide is an autocrine inhibitor of mitosis in cultured cortical precursor cells
doi:
Figure Lengend Snippet: Characterization of E13.5 cortical precursor cultures. Cells were incubated for 3 (a, b, d, e) or 24 h (c and f) and examined by phase (a and d) or brightfield microscopy to detect neuronal markers, immunoreactive MAP2 (b and c), and NSE (e and f). Neuronal markers increase from ≈50% at 3 h (b and e) to ≈90% at 24 h (c and f) (positive staining indicated by arrows; arrowhead = negative cells). Cells were unstained when primary antibody was omitted. MAP2 and NSE analyses were performed on five and two experiments, respectively. (Bar = 50 μm.)
Article Snippet: After 4% paraformaldehyde fixation (20 min), immunocytochemistry was performed with antibodies to MAP2 (1:1,000; I. Fischer, Medical College of Philadelphia),
Techniques: Incubation, Microscopy, Staining