Journal: iScience

Article Title: Analysis of myocardial cellular gene expression during pressure overload reveals matrix based functional intercellular communication

doi: 10.1016/j.isci.2022.103965

Figure Lengend Snippet: Endothelial, fibroblast and cardiomyocyte cell purification from mouse hearts (A) Scheme on the experimental procedures to isolate the indicated cell types from mouse hearts. (B) Heatmap of cell marker gene expression in RNA from isolated cardiomyocytes (CM), endothelial cells (EC) or fibroblasts (FB) after sham or the indicated time point after TAC surgery. (C) Cardiac endothelial cells and fibroblasts were isolated from mouse hearts and stained for the endothelial markers CD31 and CD102, for the leukocyte marker CD45, and the fibroblast marker Mefsk4. Subsequently, flow cytometric analyses were performed and representative results are shown here. The numbers indicated in each quadrant indicates the percentage of cells localized in that particular quadrant. (D) RNA from the different cell types after sham or 1 and 8 weeks after TAC was subjected to RNA sequencing. The differences in overall gene expression patterns were visualized by a principal component analysis.

Article Snippet: CD31 Antibody, anti-mouse , Miltenyi Biotec , Clone 390.

Techniques: Purification, Marker, Gene Expression, Isolation, Staining, RNA Sequencing

Journal: iScience

Article Title: Analysis of myocardial cellular gene expression during pressure overload reveals matrix based functional intercellular communication

doi: 10.1016/j.isci.2022.103965

Figure Lengend Snippet:

Article Snippet: CD31 Antibody, anti-mouse , Miltenyi Biotec , Clone 390.

Techniques: Purification, Plasmid Preparation, Blocking Assay, Magnetic Beads, Recombinant, Clinical Proteomics, Protease Inhibitor, cDNA Synthesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Materials Today Bio

Article Title: Porcine decellularized splenic matrix hydrogel-based vesicle sustained-release System: A dual-regulatory biomaterial for accelerated wound healing via angiogenesis and immune remodeling

doi: 10.1016/j.mtbio.2025.102601

Figure Lengend Snippet: pDSMG/SHED-ApoVs promoted skin wound healing in vivo. (A) Photographs of the skin wounds treated with pDSMG/SHED-ApoVs, pDSMG, SHED-ApoVs and PBS. (B) Traces of wound closure of each treatment. (C) Wound healing rate at different time points post-wounding (n = 5). ∗: pDSMG/SHED-ApoVs vs. pDSMG, SHED-ApoVs and PBS groups; # pDSMG vs. SHED-ApoVs and PBS groups; +: SHED-ApoVs vs. PBS groups. (D) H&E staining of tissue sections on day 10. White arrow: regenerated hair follicles and neo-epithelia; black arrow: defect area. (E) Representative Masson staining images of tissue sections on day 10. White arrow: regenerated hair follicles and neo-epithelia; yellow arrow: defect area. Quantification analysis of the width of wounded tissues without hair follicle (F), the number of hair follicles (G), the thickness of the regenerated epithelium (H) and the relative collage area (I) in the wounded tissues on day 10 (n = 5). (J) Representative IF staining images of CD31 + α-SMA + blood vessels in wound regions. CD31: green; α-SMA: red. White arrow: co-localization of CD31 and α-SMA. (K–L) Quantification of the relative area (n = 9) and the number of blood vessels (n = 5) in wound regions. Panels (F–I), (K) and (L) are designated by the same identifier. All data were presented as the means ± SD. Statistical analysis was performed by one-way ANOVA. ns, no significant; ∗ # +, P < 0.05; ∗∗ ## ++, P < 0.01; ∗∗∗ ### +++, P < 0.001; ∗∗∗∗ #### ++++, P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The antibodies used were as follows: Anti-CD31 antibody (FAB3628G, R&D Systems, USA; diluted 1:100), Anti-alpha-smooth muscle actin (α-SMA) antibody (ab7817, Abcam, UK; diluted 1:100), Anti-EMCN antibody (14-5851-82, Invitrogen, USA; diluted 1:200), Anti-F4/80 antibody (ab16911, Abcam, UK; diluted 1:200), Anti-iNOS antibody (ab178945, Abcam, UK; diluted 1:200), Anti-IL-6 antibody (12912s, Cell Signaling Technology, USA; diluted 1:200), Anti- Arginase 1(Arg1) antibody (93668s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD4 antibody (11948s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD25 antibody (11948s, Cell Signaling Technology, USA; diluted 1:200), Anti-Foxp3 antibody (22228-1-AP, Proteintech, China; diluted 1:200), Anti-TGF-β antibody (3711s, Cell Signaling Technology, USA; diluted 1:200), Anti-TNF-α, antibody (SC-52746, Santa Cruz Biotechnology, USA; diluted 1:200), Anti-collagen type I alpha 1 chain ( COL1A1 ) antibody (# P02452 , Cell Signaling Technology, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Mouse secondary antibody (A21203, Invitrogen, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Rat secondary antibody (A21209, Invitrogen, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Rabbit secondary antibody (A21207, Invitrogen, USA; diluted 1:200), Alexa Fluor 488 Donkey anti-Rabbit secondary antibody (A21206, Invitrogen, USA; diluted 1:200)

Techniques: In Vivo, Staining

Journal: Materials Today Bio

Article Title: Porcine decellularized splenic matrix hydrogel-based vesicle sustained-release System: A dual-regulatory biomaterial for accelerated wound healing via angiogenesis and immune remodeling

doi: 10.1016/j.mtbio.2025.102601

Figure Lengend Snippet: pDSMG/SHED-ApoVs promoted re-epithelialization and angiogenesis at early phase of wound healing. (A) Photographs of treated wounds at 4 days after surgery and quantification of the wound healing rate (n = 5). (B) H&E and Masson staining of wound regions and quantification of the length of neo-epithelia on day 4 (n = 5). Yellow line: neo-epithelia; Black line: the edge of wound area. (C) Schematic diagram of the cell scratch assay process. (D) Representative images of scratch assay of HaCaT pre-incubated with different groups. (E) Quantification of the migration rate of HaCaT (n = 9). (F) Representative IF staining images of CD31 + EMCN + blood vessels in wound regions. CD31: green; EMCN: red (G) Quantification of the relative area of blood vessels (n = 9) and the numbers of blood vessels in wounded areas (n = 5). All data were presented as the means ± SD. Statistical analysis was performed by one-way ANOVA. ns, no significant; ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The antibodies used were as follows: Anti-CD31 antibody (FAB3628G, R&D Systems, USA; diluted 1:100), Anti-alpha-smooth muscle actin (α-SMA) antibody (ab7817, Abcam, UK; diluted 1:100), Anti-EMCN antibody (14-5851-82, Invitrogen, USA; diluted 1:200), Anti-F4/80 antibody (ab16911, Abcam, UK; diluted 1:200), Anti-iNOS antibody (ab178945, Abcam, UK; diluted 1:200), Anti-IL-6 antibody (12912s, Cell Signaling Technology, USA; diluted 1:200), Anti- Arginase 1(Arg1) antibody (93668s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD4 antibody (11948s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD25 antibody (11948s, Cell Signaling Technology, USA; diluted 1:200), Anti-Foxp3 antibody (22228-1-AP, Proteintech, China; diluted 1:200), Anti-TGF-β antibody (3711s, Cell Signaling Technology, USA; diluted 1:200), Anti-TNF-α, antibody (SC-52746, Santa Cruz Biotechnology, USA; diluted 1:200), Anti-collagen type I alpha 1 chain ( COL1A1 ) antibody (# P02452 , Cell Signaling Technology, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Mouse secondary antibody (A21203, Invitrogen, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Rat secondary antibody (A21209, Invitrogen, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Rabbit secondary antibody (A21207, Invitrogen, USA; diluted 1:200), Alexa Fluor 488 Donkey anti-Rabbit secondary antibody (A21206, Invitrogen, USA; diluted 1:200)

Techniques: Staining, Wound Healing Assay, Incubation, Migration

Journal: Materials Today Bio

Article Title: Porcine decellularized splenic matrix hydrogel-based vesicle sustained-release System: A dual-regulatory biomaterial for accelerated wound healing via angiogenesis and immune remodeling

doi: 10.1016/j.mtbio.2025.102601

Figure Lengend Snippet: pDSMG/ApoVs accelerated wound healing in proliferative phase. (A) Photographs of treated wounds and quantification of the wound healing rate at 7 days post-injury (n = 5). (B) H&E staining of wound regions on day 7 and quantification of the length of neo-epithelia (n = 5). Black arrow: regenerated hair follicles; Red arrow: newly formed vesicles; Yellow line: stratum basale. (C) H&E staining results of the regions marked in the box above. The left images: the edge of the skin wound area. The right images: the center of the skin wound area. (D) Masson staining of wound regions. The left images: the edge of the skin wound area. The right images: the center of the skin wound area. (E) Quantification of the number of hair follicles, the epithelial thickness, the width of wounded tissues without hair follicles and the relative regenerative collagen area in wound regions on day 7 (n = 5). (F) Representative IF staining images of CD31 (green) and α-SMA (red) staining in wound regions on day 4. (G) Representative IF staining images of CD31 (green) and α-SMA (red) staining in wound regions on day 7. (H) Quantification of the number of CD31 + α-SMA+blood vessels in wound regions on day 4 (n = 5). (I) Quantification of the number of CD31 + α-SMA+blood vessels in wound regions on day 7 (n = 5). (J) Numbers of blood vessels at different time points post-wounding (n = 5). ∗: pDSMG/SHED-ApoVs vs. pDSMG, SHED-ApoVs and PBS groups; # pDSMG vs. SHED-ApoVs and PBS groups; +: SHED-ApoVs vs. PBS groups. Panels (E) and (H–J) are designated by the same identifier. All data were presented as the means ± SD. Statistical analysis was performed by one-way ANOVA. ns, no significant; ∗ # +, P < 0.05; ∗∗ ## ++, P < 0.01; ∗∗∗ ### +++, P < 0.001; ∗∗∗∗ #### ++++, P < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The antibodies used were as follows: Anti-CD31 antibody (FAB3628G, R&D Systems, USA; diluted 1:100), Anti-alpha-smooth muscle actin (α-SMA) antibody (ab7817, Abcam, UK; diluted 1:100), Anti-EMCN antibody (14-5851-82, Invitrogen, USA; diluted 1:200), Anti-F4/80 antibody (ab16911, Abcam, UK; diluted 1:200), Anti-iNOS antibody (ab178945, Abcam, UK; diluted 1:200), Anti-IL-6 antibody (12912s, Cell Signaling Technology, USA; diluted 1:200), Anti- Arginase 1(Arg1) antibody (93668s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD4 antibody (11948s, Cell Signaling Technology, USA; diluted 1:200), Anti-CD25 antibody (11948s, Cell Signaling Technology, USA; diluted 1:200), Anti-Foxp3 antibody (22228-1-AP, Proteintech, China; diluted 1:200), Anti-TGF-β antibody (3711s, Cell Signaling Technology, USA; diluted 1:200), Anti-TNF-α, antibody (SC-52746, Santa Cruz Biotechnology, USA; diluted 1:200), Anti-collagen type I alpha 1 chain ( COL1A1 ) antibody (# P02452 , Cell Signaling Technology, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Mouse secondary antibody (A21203, Invitrogen, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Rat secondary antibody (A21209, Invitrogen, USA; diluted 1:200), Alexa Fluor 594 Donkey anti-Rabbit secondary antibody (A21207, Invitrogen, USA; diluted 1:200), Alexa Fluor 488 Donkey anti-Rabbit secondary antibody (A21206, Invitrogen, USA; diluted 1:200)

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Vascular Remodeling of Clinically Used Patches and Decellularized Pericardial Matrices Recellularized with Autologous or Allogeneic Cells in a Porcine Carotid Artery Model

doi: 10.3390/ijms23063310

Figure Lengend Snippet: Flow cytometry analysis of isolated porcine Wharton´s jelly mesenchymal stem cells (pWJCs).

Article Snippet: 31 , 0.26% , Origene (Rockville, MD, USA) , SM2146APC.

Techniques: Flow Cytometry, Isolation, Marker