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Image Search Results
Journal: Plant Physiology
Article Title: Kinase Partner Protein Plays a Key Role in Controlling the Speed and Shape of Pollen Tube Growth in Tomato
doi: 10.1104/pp.20.01081
Figure Lengend Snippet: KPP preferentially activates ROP4 and interacts with ROP4 at the plasma membrane of pollen tubes. A and B, GEF activity assay. A, Time course of exchanged guanine nucleotide amounts of various ROPs with or without KPPΔN8. B, Time course of exchanged guanine nucleotide amounts of ROP4 with various truncated fragments of KPP. Results shown are from one of three independent assays that gave similar results. Rate constants (Kobs) are shown in parentheses. Kobs values were obtained by monophasic exponential fits using PeakFit v4.01. C to E, Representative pollen tubes coexpressing BiFC constructs YC-ROP4 with KPP-YN (C), KPPΔN45-YN (D), and KPPΔN8-YN (E). F, Transient overexpression of eGFP-ROP4 in tobacco pollen tubes. BF, Bright field. Bars = 20 μm.
Article Snippet: The K obs values (pseudo-first-order rate constant) were obtained by monophasic-exponential fits using
Techniques: Activity Assay, Construct, Over Expression