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Image Search Results
Journal: Oncotarget
Article Title: VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells
doi: 10.18632/oncotarget.24078
Figure Lengend Snippet: ( A ) Schematic of CAR constructs containing VEGFR2-specific Fn3-based antigen-recognition module. CARs obtained encompass leader sequences from either mIgk or Gaussia princeps luciferase (Gluc), VEGFR2-specific Fn3 sequence (VR2 FN3), myc epitope tag, hIgG1 spacer region (hinge-CH2-CH3 domains), CD28 region (transmembrane and signaling sequences), and CD3ζ region (transmembrane and/or signaling sequences). The vertical black line denotes the cell membrane. ( B ) FACS detection of VEGFR2 expression on the surface of HEK293T(VEGFR2+) cells stained with either recombinant FLAG-tagged Fn(VEGFR2)—VR2 FN3, FLAG-tagged Fn3 of irrelevant specificity—CEA FN3 , or left unstained. ( C ) Western blot detection of FnCAR expression in transduced Jurkat cells (anti-myc). ( D ) flow cytometry surface staining of kVR2-28z FnCAR-expressing Jurkat cells (becoming copGFP+ upon transduction) with anti-hinge (IgG-specific APC-labeled) conjugates. ( E ) Expression of the activation marker CD69 on CAR-Jurkat cells incubated with HEK293T(VEGFR2+) target cells or isogenic control cells (HEK293T) for the times indicated.
Article Snippet: Immediately after incubation, the cells were stained with
Techniques: Construct, Luciferase, Sequencing, Membrane, Expressing, Staining, Recombinant, Western Blot, Flow Cytometry, Transduction, Labeling, Activation Assay, Marker, Incubation, Control
Journal: Oncotarget
Article Title: VEGFR2-specific FnCAR effectively redirects the cytotoxic activity of T cells and YT NK cells
doi: 10.18632/oncotarget.24078
Figure Lengend Snippet: ( A ) Flow cytometry detection of CAR expression on the surface of transduced FnCAR T cells, as assayed by anti-myc staining. ( B ) VEGFR2-specific FnCAR-T cells but not irrelevant CAR-T cells (gated by the expression of CAR) become activated (CD69+) upon incubation with target PC3(VEGFR2+) cells. ( C ) PC3(VEGFR2+) target cell killing by VEGFR2-specific FnCAR-T cells (note that only ~30% of effector T cell population is FnCAR-positive, (A)), but not by irrelevant CD20-specific k20-28z CAR-T cells.
Article Snippet: Immediately after incubation, the cells were stained with
Techniques: Flow Cytometry, Expressing, Staining, Incubation