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Bioss
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Miltenyi Biotec
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OriGene
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Proteintech
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ProSci Incorporated
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Biorbyt
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fluidigm
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NanoCarrier Co
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Verlag GmbH
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PRODESIGN GmbH
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Image Search Results
Journal: Journal of Clinical Medicine
Article Title: A New Histology-Based Prognostic Index for Acute Lymphoblastic Leukemia: Preliminary Results of the “ALL Urayasu Classification”
doi: 10.3390/jcm15020768
Figure Lengend Snippet: Overall survival rate of ALL patients with and without prognostic factors. Kaplan–Meier (KM)survival curves and prognostic factors were compared between groups using the log-rank test (red indicates immunostaining-positive groups, black indicates immunostaining-negative groups. 95% confidence intervals (CI) have been added to all KM graphs. ( A ) OS was significantly shortened in the AKR1B10-positive group ( p < 0.05). ( B ) OS was significantly shortened in the AKR1C3-positive group ( p < 0.05). ( C ) There was no significant difference in OS between the AKR1B1-positive and -negative groups (NS). ( D ) There was no significant difference in OS rate between the MRP1-positive and -negative groups. ( E ) There was no significant difference in OS between the PD-L2-positive and -negative groups. ( F ) There was no significant difference in OS between the CYP2B6-positive and -negative groups. It is known that the log-rank test, a common test for KM curves, does not allow for reliable statistical conclusions because the sample size is too small. Therefore, we retested and using the Pet-Pet-Wilcoxon method, which is often used for small samples. The results were generally the same, except for MRP1 in D, which changed from NS to p < 0.05. However, this change did not have much impact on the overall discussion.
Article Snippet: Primary antibodies against key proteins involved in anticancer drug metabolism were used: (1) GRP94: Proteintech (Rosemont, IL, USA), clone 1H10B7 (monoclonal antibody); (2) CYP3A4: Sigma-Aldrich (St. Louis, MO, USA), SAB1400064 (polyclonal antibody against CYP3A4); (3) AKR1C3: Proteintech 11194-1-AP (polyclonal antibody against AKR1C3); (4) MDR1 (P-glycoprotein): Proteintech, 22336-1-AP (polyclonal antibody against MDR1); (5) MRP1 (CD9): Proteintech, 60232-1-IG (monoclonal antibody against full-length MRP1); (6) TGF-beta1: Proteintech, 21898-1-AP (polyclonal antibody against TGF-beta); (7) GRP78: Proteintech, 66574-1-IG (monoclonal antibody against full-length GRP78); (8) Glutathione S-transferase-κ1 (GST): Proteintech, 14535-1-AP (polyclonal antibody against GST1); (9) Thymidine phosphorylase: Abcam (Cambridge, UK), ab226917 (polyclonal antibody); (10) MRP4 (ABCC4): SANTA CRUZ BIOTECHNOLOGY (Dallas, TX, USA), SC-376262 (monoclonal antibody against MRP4); (11) CYP2B6: LifeSpan BioSciences, Inc. (Seattle, WA, USA), LS-C352084 (polyclonal antibody against CYP2B6); (12) TNF1-alpha: Sigma-Aldrich, SAB4502982 (polyclonal antibody against TNF1-alpha) (13) PD-1; (14) PD-L1: Proteintech, 66248-1-IG, monoclonal antibody, clone 2B11D11; (15)
Techniques: Immunostaining
Journal: Frontiers in Immunology
Article Title: CMTM6-Deficient Monocytes in ANCA-Associated Vasculitis Fail to Present the Immune Checkpoint PD-L1
doi: 10.3389/fimmu.2021.673912
Figure Lengend Snippet: Less PD-L1+ monocytes in patients with AAV. (A) Percentages of PD-L1+ monocytes in HC (n=20) and AAV patients (n=26). (B) Surface expression of PD-L1 (MFI) on classical (CD14+CD16-), intermediate (CD14+CD16+), and non-classical monocytes (CD14dim CD16++) in healthy control donors (n=20) and AAV patients (n=26). (C–E) Percentages of PD-L2+, CD80+, and CD86+ monocytes in HC and AAV patients (n=9-10 each cohort). Mann-Whitney (A, B, D) test or unpaired t-test (C, E) were applied. **P<0.01; ***P<0.001. Bar graph shows mean ± SEM. AAV, ANCA-associated vasculitis; HC, healthy control donors; MFI, mean fluorescence intensity; PD-L1, Programmed death-ligand 1; ns, statistically not significant.
Article Snippet: The following antibodies or stainings were used: CMTM6 (
Techniques: Expressing, Control, MANN-WHITNEY, Fluorescence