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Thermo Fisher
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Proteintech
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OriGene
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Thermo Fisher
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Thermo Fisher
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Eupheria Biotech GmbH
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Promega
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ABclonal Biotechnology
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Image Search Results
Journal: Cell metabolism
Article Title: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue
doi: 10.1016/j.cmet.2022.02.016
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Immunofluorescence, Recombinant, Cytometry, Purification, Virus, Control, shRNA, Liposomes, Cell Culture, Red Blood Cell Lysis, XF Assay, Staining, Isolation, Transfection, Live Cell Imaging, Mouse Assay, Software, Gene Expression
Journal: Journal of Cellular and Molecular Medicine
Article Title: Perfusion of isolated rat kidney with Mesenchymal Stromal Cells/Extracellular Vesicles prevents ischaemic injury
doi: 10.1111/jcmm.13249
Figure Lengend Snippet: mRNA expression of cell energy metabolism and membrane transport genes validated by RT‐PCR in BS, MSC and EV groups. Columns show gene mRNA expression normalized to beta‐actin expression and converted to fold change. Data are means ± S.D. The groups are defined in Figure . Panel A . Isocitrate dehydrogenase 2 (Idh2) mRNA expression (° P < 0.005 versus BS; * P < 0.05 versus EV and BS). Panel B . NADH dehydrogenase (ubiquinone), Fe‐S protein 8 (Ndufs8) mRNA expression (° P < 0.005 versus BS; * P < 0.05 versus BS). Panel C . Pyruvate dehydrogenase beta (Pdhb) mRNA expression (* P < 0.05 versus BS; ° P < 0.01 versus BS). Panel D . Calbindin1 (Calb1) mRNA expression (° P < 0.05 versus BS). Panel E . Monocarboxylate transporter 1 (Slc16a1) mRNA expression (° P < 0.05 versus BS and MSC). Panel F . Vacuolar H + ‐ATPase d2 Subunit (Atp6v0d2) mRNA expression (° P < 0.01 versus BS and MSC).
Article Snippet: A total of 1 μg of RNA per condition was reverse transcribed into cDNA with the 1st Strand cDNA Synthesis Kit for RT‐PCR (AMV) (Roche Applied Science, Penzberg, Germany). cDNA was used to perform the real‐time PCR analyses in 96‐well optical reaction plates, using ABI prism 5700 (Applied Biosystems, Waltham, MA, USA) and the 5‐exonuclease assay (TaqMan technology) in a 10 μl reaction volume containing TaqMan Universal Master Mix, optimized concentrations of FAM‐labelled probes for B2 m, Idh2, Ndufs8, Pdhb, Calb1, Slc16a1 and Atp6v0d2 (Applied Biosystems, catalogue numbers Hs00187842_m1, Hs00158033_m1, Hs00159597_m1,
Techniques: Expressing, Membrane, Reverse Transcription Polymerase Chain Reaction
Journal: International Journal of Molecular Sciences
Article Title: Differential Impact of Zearalenone on Hepatic Glucose and Lipid Metabolism in Healthy and Ketotic Dairy Cows: An In Vitro Study
doi: 10.3390/ijms26146827
Figure Lengend Snippet: Relative mRNA expression of PDHB normalized to GAPDH in hepatocyte cultures derived from healthy and ketotic cows after exposure to Zearalenone (ZEN) for ( a ) 1 h, ( b ) 3 h, and ( c ) 6 h. Bars represent the mean relative quantity (RQ) of mRNA expression in control (C; 0 μM ZEN) and treatment groups (10, 20, 50, 100 μM). The expression in untreated control samples (C) was used as the calibrator for each group. Asterisks indicate statistically significant differences compared to the control within the same group: * p < 0.05, ** p < 0.001. Error bars represent the standard error of the mean (SEM).
Article Snippet: The resulting pre-amplified cDNA preparations were analyzed by real-time PCR in a StepOnePlus Real-time PCR System (
Techniques: Expressing, Derivative Assay, Control