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Image Search Results
Journal: PLoS ONE
Article Title: IFNγ-stimulated dendritic cell extracellular vesicles can be nasally administered to the brain and enter oligodendrocytes
doi: 10.1371/journal.pone.0255778
Figure Lengend Snippet: IFNγ-DC-EVs were transfected with reporter mCherry mRNA. (A) Nasal delivery of transfected EVs (NA mCherry-EV) confirmed EV delivery of functional mCherry mRNA to brain cells. Representative images show mCherry fluorescence (red), anti-mCherry immunostaining (green) and merged image (yellow) to confirm mCherry expression in cells. Animals that were nasally administered untransfected EVs (Sham) showed no mCherry expression/staining. Scale bar = 25 μm. (B) Representative merged images (B1-8) show widespread cellular distribution in the CNS two days after nasal administration of mCherry mRNA transfected IFNγ-DC-EVs. Images were taken from brain sections represented in the schematic. A subset of mCherry-positive cells colocalize with CNPase, indicating that they are oligodendrocytes. (C) Representative high power images of mCherry expression (red) in cells that co-stained (yellow) for CNPase (green). Scale bar = 25 μm. (D) Similarly, stained representative merged images (D1-8) show oligodendrocyte co-staining throughout the CNS, with numbers corresponding to brain areas in schematic (B). Scale bar = 20 μm. ( n = 5-7/group; A-D). To confirm that IFNγ-DC-EVs delivered cargo to oligodendrocytes, protein expression levels of known miR-219 mRNA targets expressed in oligodendrocyte lineage cells were quantified via immunoblot. Three days after nasal delivery of IFNγ-DC-EVs or Unstim-DC-EVs [Sham(S)], levels of (E) PDGFRα, and (F) ELOVL7 were significantly (* p = 0.040 and 0.038 respectively) reduced in the brains of animals that received IFNγ-DC-EVs. ( n = 3-6/group).
Article Snippet: Primary antibodies used were
Techniques: Transfection, Functional Assay, Fluorescence, Immunostaining, Expressing, Staining, Western Blot