Journal: CNS neuroscience & therapeutics

Article Title: Effects of the Small-Molecule ISRIB on the Rapid and Efficient Myelination of Oligodendrocytes in Human Stem Cell-Derived Cerebral Organoids in Patients With Leukoencephalopathy With Vanishing White Matter.

doi: 10.1111/cns.70398

Figure Lengend Snippet: FIGURE 1 | Normal OL brain organoids displayed maturation of astrocytes, oligodendrocytes, and myelin sheaths. (A) Bright-field images under the microscope of the rapid OL organoids at different days of maturation. (B) Immunofluorescence staining for Nestin (green) and nuclear staining (DAPI, blue) at Week 2 and Week 4. Immunofluorescence staining for GFAP (green), S100B (red), PDGFRα (green), Olig2 (red), and nuclear staining (DAPI, blue) at Week 6 and Week 8. Immunofluorescence staining for MB P (red) at Week 8 and Week 10. Scale bars 100 μm. (C) The diameter of normal organoids at Week 8 and Week 10. (D) Transmission electron microscopy showed the myelin sheath. Scale bars 1 μm.

Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 13 CNS Neuroscience & Therapeutics, 2025 1:300, 3670 S), PDGFRα (Santa Cruz, 1:200, ab61219), Olig2 (Abcam, 1:100, ab109186), myelin basic protein (MBP, CST, 1:100, ab62631), GFAPδ (EMD Millipore, 1:500, AB9598), nestin (CST, 1:200, 4760 S), αB- crystallin (Abcam, 1:200, ab76467), O4 (Sigma–Aldrich, 1:100, MAB1326), ATF4 (CST, 1:100, 11,815 S), ATF6 (Abcam, 1:100, ab122897), and XBP1- S (CST, 1:100).

Techniques: Microscopy, Immunofluorescence, Staining, Transmission Assay, Electron Microscopy

Journal: CNS neuroscience & therapeutics

Article Title: Effects of the Small-Molecule ISRIB on the Rapid and Efficient Myelination of Oligodendrocytes in Human Stem Cell-Derived Cerebral Organoids in Patients With Leukoencephalopathy With Vanishing White Matter.

doi: 10.1111/cns.70398

Figure Lengend Snippet: FIGURE 3 | eIF2B mutation resulted in decreased OPCs, mature oligodendrocytes, and sparse myelin. (A) Immunofluorescence staining for OPC marker PDGFRα (green), mature oligodendrocytes marker Olig2 (red), and nuclear staining (DAPI, blue) at Week 8. Scale bars 100 μm. (B) Immunofluorescence staining for immature oligodendrocyte marker O4 (green) and nuclear staining (DAPI, blue) at Week 8. Scale bars 100 μm. (C) Immunofluorescence staining for mature myelin marker MBP (red) and nuclear staining (DAPI, blue) at Week 10. (D,E) Transmission electron microscopy showed helical or concentrically coiled myelin-like structures in the WT organoids and the sparse myelin sheaths in mutant organoids. Scale bars 1 μm. (F–I) Data were presented as mean ± SEM values, n = 8 in each group with three independent experiments; one-way ANOVA analy- sis and Kruskal–Wallis ANOVA analysis. ns: p > 0.05, **p < 0.01, ***p < 0.001. (J) Quantification of G-ratio at Week 10 using one-way ANOVA analy- sis and Kruskal–Wallis ANOVA analysis. ns: p > 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 13 CNS Neuroscience & Therapeutics, 2025 1:300, 3670 S), PDGFRα (Santa Cruz, 1:200, ab61219), Olig2 (Abcam, 1:100, ab109186), myelin basic protein (MBP, CST, 1:100, ab62631), GFAPδ (EMD Millipore, 1:500, AB9598), nestin (CST, 1:200, 4760 S), αB- crystallin (Abcam, 1:200, ab76467), O4 (Sigma–Aldrich, 1:100, MAB1326), ATF4 (CST, 1:100, 11,815 S), ATF6 (Abcam, 1:100, ab122897), and XBP1- S (CST, 1:100).

Techniques: Mutagenesis, Immunofluorescence, Staining, Marker, Transmission Assay, Electron Microscopy

Journal: CNS neuroscience & therapeutics

Article Title: Effects of the Small-Molecule ISRIB on the Rapid and Efficient Myelination of Oligodendrocytes in Human Stem Cell-Derived Cerebral Organoids in Patients With Leukoencephalopathy With Vanishing White Matter.

doi: 10.1111/cns.70398

Figure Lengend Snippet: FIGURE 6 | ISRIB treatment increased mature oligodendrocytes and myelin in mutant OL cerebral organoids. (A,B) Immunofluorescence stain- ing for OPC marker PDGFRα (green) and mature oligodendrocytes marker Olig2 (red) and nuclear staining (DAPI, blue) without ISRIB and ISRIB treatment (500 μm), respectively, at Week 8. Scale bars 100 μm. (C,D) Immunofluorescence staining for mature oligodendrocyte marker O4 (green) and nuclear staining (DAPI, blue), without ISRIB and ISRIB treatment (500 μm), respectively, at Week 8. Scale bars 100 μm. (E,F) Immunofluorescence staining for mature myelin marker MBP (red) and nuclear staining (DAPI, blue) without ISRIB and ISRIB treatment (500 μm), respectively, at Week 10. (G,H) Transmission electron microscopy showed the helical myelin-like structure in the WT and mutant organoids after ISRIB treatment (500 μm) at Week 10. Scale bars 1 μm. (I–L) Data were presented as mean ± SEM values, n = 8 in each group with three independent experiments; one- way ANOVA analysis and Kruskal–Wallis ANOVA analysis. ns: p > 0.05, **p < 0.01, ***p < 0.001. (M) Quantification of G-ratio after ISRIB treatment at Week 10; one-way ANOVA analysis and Kruskal–Wallis ANOVA analysis. ns: p > 0.05, **p < 0.01, ***: p < 0.001.

Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 13 CNS Neuroscience & Therapeutics, 2025 1:300, 3670 S), PDGFRα (Santa Cruz, 1:200, ab61219), Olig2 (Abcam, 1:100, ab109186), myelin basic protein (MBP, CST, 1:100, ab62631), GFAPδ (EMD Millipore, 1:500, AB9598), nestin (CST, 1:200, 4760 S), αB- crystallin (Abcam, 1:200, ab76467), O4 (Sigma–Aldrich, 1:100, MAB1326), ATF4 (CST, 1:100, 11,815 S), ATF6 (Abcam, 1:100, ab122897), and XBP1- S (CST, 1:100).

Techniques: Mutagenesis, Immunofluorescence, Staining, Marker, Transmission Assay, Electron Microscopy

Journal: Cell reports

Article Title: Human Glial Progenitor Cells Effectively Remyelinate the Demyelinated Adult Brain

doi: 10.1016/j.celrep.2020.107658

Figure Lengend Snippet: Human glial chimeras were maintained on either a cuprizone (CZN)-containing or control diet from 12–24 weeks of age. Twelve weeks later, at 36 weeks, the mice were killed and their resident hGPCs isolated by CD140a-based FACS, which were then subjected to RNA-seq (n = 6).

Article Snippet: AB_396285 CD140a-PE PE-conj mouse anti-human CD140a, AR1 10 μL/ 10 6 cells 556002 BD Biosci AB_396286 Secondaries Rat anti-Mouse IgM MicroBeads 20 μL/10 7 cells 130-047-301 Miltenyi AB_244358 Rat anti-Mouse IgG2a+b MicroBeads 10 μL/10 7 cells 130-047-201 Miltenyi AB_244356 AlexaFluor 568 Goat anti-Mouse IgG (H+L) 1:400 A-11031 Invitrogen AB_144696 AlexaFluor 568 Goat anti-Mouse IgG1 1:400 A-21124 Invitrogen AB_2535766 AlexaFluor 488 Goat anti-Mouse IgG (H+L) 1:400 A-11029 Invitrogen AB_2534088 AlexaFluor 488 Goat anti-Mouse IgG1 1:400 A-21121 Invitrogen AB_2535764 AlexaFluor 647 Goat anti-Mouse IgG (H+L) 1:400 A-21235 Jackson AB_2535813 AlexaFluor 568 Goat anti-Rabbit IgG (H+L) 1:400 A-11036 Invitrogen AB_2534094 AlexaFluor 488 Goat anti-Rabbit IgG (H+L) 1:400 A-11034 Invitrogen AB_2576217 AlexaFluor 568 Goat anti-Rat IgG (H+L) 1:400 A-11077 Invitrogen AB_2534121 AlexaFluor 488 Goat anti-Rat IgG (H+L) 1:400 A-11006 Invitrogen AB_2534074 Open in a separate window

Techniques: Control, Isolation, RNA Sequencing

Journal: Cell reports

Article Title: Human Glial Progenitor Cells Effectively Remyelinate the Demyelinated Adult Brain

doi: 10.1016/j.celrep.2020.107658

Figure Lengend Snippet:

Article Snippet: AB_396285 CD140a-PE PE-conj mouse anti-human CD140a, AR1 10 μL/ 10 6 cells 556002 BD Biosci AB_396286 Secondaries Rat anti-Mouse IgM MicroBeads 20 μL/10 7 cells 130-047-301 Miltenyi AB_244358 Rat anti-Mouse IgG2a+b MicroBeads 10 μL/10 7 cells 130-047-201 Miltenyi AB_244356 AlexaFluor 568 Goat anti-Mouse IgG (H+L) 1:400 A-11031 Invitrogen AB_144696 AlexaFluor 568 Goat anti-Mouse IgG1 1:400 A-21124 Invitrogen AB_2535766 AlexaFluor 488 Goat anti-Mouse IgG (H+L) 1:400 A-11029 Invitrogen AB_2534088 AlexaFluor 488 Goat anti-Mouse IgG1 1:400 A-21121 Invitrogen AB_2535764 AlexaFluor 647 Goat anti-Mouse IgG (H+L) 1:400 A-21235 Jackson AB_2535813 AlexaFluor 568 Goat anti-Rabbit IgG (H+L) 1:400 A-11036 Invitrogen AB_2534094 AlexaFluor 488 Goat anti-Rabbit IgG (H+L) 1:400 A-11034 Invitrogen AB_2576217 AlexaFluor 568 Goat anti-Rat IgG (H+L) 1:400 A-11077 Invitrogen AB_2534121 AlexaFluor 488 Goat anti-Rat IgG (H+L) 1:400 A-11006 Invitrogen AB_2534074 Open in a separate window

Techniques: In Vivo, In Vitro

Journal: Cell reports

Article Title: Human Glial Progenitor Cells Effectively Remyelinate the Demyelinated Adult Brain

doi: 10.1016/j.celrep.2020.107658

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: AB_396285 CD140a-PE PE-conj mouse anti-human CD140a, AR1 10 μL/ 10 6 cells 556002 BD Biosci AB_396286 Secondaries Rat anti-Mouse IgM MicroBeads 20 μL/10 7 cells 130-047-301 Miltenyi AB_244358 Rat anti-Mouse IgG2a+b MicroBeads 10 μL/10 7 cells 130-047-201 Miltenyi AB_244356 AlexaFluor 568 Goat anti-Mouse IgG (H+L) 1:400 A-11031 Invitrogen AB_144696 AlexaFluor 568 Goat anti-Mouse IgG1 1:400 A-21124 Invitrogen AB_2535766 AlexaFluor 488 Goat anti-Mouse IgG (H+L) 1:400 A-11029 Invitrogen AB_2534088 AlexaFluor 488 Goat anti-Mouse IgG1 1:400 A-21121 Invitrogen AB_2535764 AlexaFluor 647 Goat anti-Mouse IgG (H+L) 1:400 A-21235 Jackson AB_2535813 AlexaFluor 568 Goat anti-Rabbit IgG (H+L) 1:400 A-11036 Invitrogen AB_2534094 AlexaFluor 488 Goat anti-Rabbit IgG (H+L) 1:400 A-11034 Invitrogen AB_2576217 AlexaFluor 568 Goat anti-Rat IgG (H+L) 1:400 A-11077 Invitrogen AB_2534121 AlexaFluor 488 Goat anti-Rat IgG (H+L) 1:400 A-11006 Invitrogen AB_2534074 Open in a separate window

Techniques: Marker, In Vitro, Recombinant, Clinical Proteomics, Software, Microscopy, Lysis