Journal: iScience

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle

doi: 10.1016/j.isci.2023.107479

Figure Lengend Snippet: Use of Pdgfra expression to identify FAP-specific gene expression (A–C) UMAP of in silico sorted FAPs using A) SCA-1/ Ly6a /SCA-1 FACS strategy, B) Pdgrfa in silico FACS strategy and C) a combination of both. (D) Heatmap of the top FAP expressing genes comparing Pdgfra (+), Pdgfra (−) and other cell-types. (E) Bubble plot of top 20 upregulated biological processes in Pdgfra expressing cells compared to all other mononuclear cell types (GSEA). (F) Networks of related biological processes up- and downregulated in FAPs generated using Cytoscape/EnrichmentMap.

Article Snippet: Pdgfra - Mm00440701_m1 (FAM-MGB) , Thermo Fisher , 4331182.

Techniques: Expressing, Gene Expression, In Silico, Generated

Journal: iScience

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle

doi: 10.1016/j.isci.2023.107479

Figure Lengend Snippet: Altered expression of FAP-specific genes in LGMD2B mouse model (A) Quantitative PCR analysis of relative expression of FAP-specific genes in 12-month-old WT and 3-month or 12-month-old BLA/J mouse quadriceps muscles (∗ = p value <0.05). Error bars indicate standard deviation. (B) Confocal microscope images showing in situ distribution of C3, IL-33, ADAMTS5 and PDGFRα proteins in WT and BLA/J quadricep muscle. Scale bar = 50 μm. (C) Epifluorescence images showing broader tissue-wide distribution of FAP marker proteins immunostained as in panel B. Scale bar = 100 μm. (D) Confocal images showing RNA-FISH to detect C3, Il33, Adamts5, Dpep1 and Pdgfra RNAs in WT and BLA/J muscle sections. Scale bar = 15 μm.

Article Snippet: Pdgfra - Mm00440701_m1 (FAM-MGB) , Thermo Fisher , 4331182.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Muscles, Standard Deviation, Microscopy, In Situ, Marker

Journal: iScience

Article Title: Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle

doi: 10.1016/j.isci.2023.107479

Figure Lengend Snippet:

Article Snippet: Pdgfra - Mm00440701_m1 (FAM-MGB) , Thermo Fisher , 4331182.

Techniques: RNAscope, Multiplex Assay, Recombinant, Control, Generated, Software