Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 12 CD34/PDGFR-α levels in studied groups. (*: significantly different from the control group. CTRL control group, ET endurance training group, RT: resistance training group)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Control

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 13 Changes in telocytes number in response to exercise. Double-immunofluorescence labeling for CD34/PDGFR-α used to determine telocytes: these cells are increased in response to 8 weeks of endurance training (ET) and resistance training (RT)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Immunofluorescence, Labeling

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 15 Changes in telocytes number in response to exercise. Double-immunofluorescence labeling for CD34/PDGFR-β used to determine telocytes: these cells are increased in response to 8 weeks of endurance training (ET) and resistance training (RT)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Immunofluorescence, Labeling

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 14 CD34/PDGFR-β levels in studied groups. (*: significantly different from the control group. CTRL control group, ET endurance training group, RT resistance training group)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Control

Journal: Biology of reproduction

Article Title: Prenatal exposure to estrogenic compounds alters the expression pattern of platelet-derived growth factor receptors alpha and beta in neonatal rat testis: identification of gonocytes as targets of estrogen exposure.

doi: 10.1095/biolreprod.102.009605

Figure Lengend Snippet: FIG. 4. Quantification of in situ hybridization signals for PDGFRa and b mRNAs in 3-day-old testis sections. Densitometric analysis was per- formed as described in the Materials and Methods section. The histograms represent the specific intensities of signals, expressed in arbitrary units, for PDGFRa (A) and PDGFRb (B) that were present in the interstitium, periphery, and center of the seminiferous cords of the same samples as Figure 3. *P , 0.05; ***P , 0.001.

Article Snippet: Rabbit polyclonal antibodies against the Cterminal domains of PDGFRa and PDGFRb were purchased from Santa Cruz Biotechnology (Santa Cruz, CA), while rabbit polyclonal antibodies against the kinase domain of PDGFRb was purchased from BD Biosciences (BD Pharmingen, San Diego, CA).

Techniques: In Situ Hybridization

Journal: Cancers

Article Title: Tumor-Associated Fibroblast-Derived Exosomal circDennd1b Promotes Pituitary Adenoma Progression by Modulating the miR-145-5p/ONECUT2 Axis and Activating the MAPK Pathway

doi: 10.3390/cancers15133375

Figure Lengend Snippet: TAFs promoted PA progression by inhibiting miR-145-5p in PA cells. ( a ) Correlation of PA texture with PA size and aggressiveness (n = 242). ( b ) Immunohistochemical staining of TAFs indicators (α-SMA, PDGFRB, TAGLN) between soft and tough textual tumor (scale bar = 100 μm) (n = 49). ( c – e ) Correlation of TAFs density with PA size and aggressiveness. ( f ) Schematic diagram of extracting NFs and TAFs (figure created with Biorender.com). ( g ) Cell viability experiments of showing effect of TAFs supernatant on indicated PA cells (n = 6), PA1221 and PA0222 were primary PA cells. ( h ) Transwell assay of effect of TAFs supernatant on indicated PA cells (n = 6). ( i ) Analysis of tumor volume and tumor weight after indicated PA cells were mixed with NFs or TAFs (n = 6) (figure was created with Biorender.com). ( j ) Venn diagram of upregulated circRNAs and downregulated miRNAs in PA. ( k ) Heatmap of downregulated miRNAs in PA. ( l ) Correlation analysis of 5 miRNAs with TAF density (n = 15). ( m ) MiR-145-5p expression level after TAFs supernatant effect on PA cells (n = 3). ( n ) Cell viability experiments of effect of TAFs supernatant on PA cells transfected with miR-145-5p mimic (n = 6). ( o ) Colony formation assay of TAFs supernatant effect on PA cells transfected with miR-145-5p mimic (n = 3). ( p ) Analysis of tumor size and weight after miR-145-5p antagomir injection (5 nmol/3 days) (n = 6) (scale bar = 10 mm). The original magnification was ×200. Data were expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Pearson correlation and linear regression were used to compare these parameters between two variables. One-way ANOVA was used for multi-sample comparison and Student’s t -test was used for comparison between two groups. Statistical significance was considered to be indicated by a value of p < 0.05. CM: conditioned medium.

Article Snippet: The tissues were blocked by 5% BSA for 1 h; then, the primary antibodies—α-SMA (Santa Cruz, CA, USA, cat: sc-32251), dilution ratio 1:200; PDGFRB (Proteintech, Wuhan, China), dilution ratio 1:50; TAGLN (Proteintech, Wuhan, China), dilution ratio 1:20, FGFR3 (Proteintech, Wuhan, China, cat: 66954-1-Ig), dilution ratio: 1:200—were applied overnight at 4 °C.

Techniques: Immunohistochemical staining, Staining, Transwell Assay, Expressing, Transfection, Colony Assay, Injection, Comparison

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: Reagent information.

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: Concentration Assay, Injection

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: MSCs migrated toward ECs via PDGF-BB/PDGFR β in the inflammatory microenvironment. (a) Representative images of migrated hBMSCs that had received different pre-treatments or had been exposed to different inducing media. The migration capacity of hBMSCs was determined using a Transwell culture system. The quantification of migrated cells was shown as a bar graph. Scale bar, 50 μ m. ∗ P < 0.05. (b) Representative images of wound healing assays. The rate of scratch wound closure was shown as a bar graph. Scale bar, 200 μ m. ∗ P < 0.05. EGM: endothelial cell growth medium-2. EC-CM: conditioned media of endothelial cells. IEC-CM: conditioned media of endothelial cells in the context of inflammatory microenvironment; shPDGF-BB: short hairpin RNA targeting pdgfb ; shPDGFR β : shRNA targeting pdgfrb .

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: Migration, shRNA

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: Src and Akt functioned downstream of PDGFR β . (a) Representative images of migrated hBMSCs in Transwell culture systems. The quantification of migrated cells was shown as a bar graph. Data were compared with the group of IEC-CM from . Scale bar, 50 μ m. ∗ P < 0.05. (b) Representative images of wound healing assays. The rate of scratch wound closure was shown as a bar graph. Data were compared with the group of IEC-CM from . Scale bar, 200 μ m. ∗ P < 0.05. IEC-CM: conditioned media of endothelial cells in the context of inflammatory microenvironment; shPDGFR β : short hairpin RNA targeting pdgfrb ; shSrc: shRNA targeting src ; shAkt: shRNA targeting akt .

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: shRNA

Journal: Stem Cells International

Article Title: Endothelial Cells Promote Migration of Mesenchymal Stem Cells via PDGF-BB/PDGFR β -Src-Akt in the Context of Inflammatory Microenvironment upon Bone Defect

doi: 10.1155/2022/2401693

Figure Lengend Snippet: Src bridged connection between PDGFR β and Akt during ECs-induced MSCs migration. (a) Gene and protein expression of Src in migrating hBMSCs. (b) Gene and protein expression of Akt in migrating hBMSCs. EC-CM: conditioned media of ECs; IEC-CM: conditioned media of ECs in the context of inflammatory microenvironment; shPDGFR β : short hairpin RNA targeting pdgfrb ; shSrc: shRNA targeting src ; shAkt: shRNA targeting akt . ∗ P < 0.05.

Article Snippet: JNJ-10198409 , PDGFR β , 5 μ M , 1 h , Pre-treat MSCs , MedChemExpress, Monmouth Junction, NJ, USA.

Techniques: Migration, Expressing, shRNA

Journal: Journal of Translational Medicine

Article Title: Papillary thyroid cancer organoids harboring BRAF V600E mutation reveal potentially beneficial effects of BRAF inhibitor-based combination therapies

doi: 10.1186/s12967-022-03848-z

Figure Lengend Snippet: List of drugs used in this study

Article Snippet: VEGFR, PDGFRβ , Lenvatinib , MedChemExpress , HY-10981 , 10.

Techniques: DNA Synthesis

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Expression of PDGF receptors in proliferating and involuting hemangiomas. ( a ) Real-time RT-PCR analysis of PDGFR- α , - β , and PPAR γ in proliferating and involuting hemangiomas (data normalized to 18S rRNA and presented as relative to normal skin; * P <0.05 compared with normal skin, † P <0.05 compared with proliferating hemangioma; n =3). ( b ) Immunostaining of proliferating hemangiomas for PDGFR- α and PDGFR- β (images taken at × 20, inserts show higher magnification; brown=DAB staining, blue=hematoxylin). ( c ) Immunofluorescence double labeling for mesenchymal cell marker α -SMA and PDGFR- β (images taken at × 20, green=PDGFR- β , red= α -SMA)

Article Snippet: The cells were transfected with control shRNA (sc-108060; Santa Cruz Biotechnology), PDGFR- α shRNA (sc-29443-SH; Santa Cruz Biotechnology) plasmid DNA, or PDGFR- β shRNA (sc-29442-SH; Santa Cruz Biotechnology) at a concentration of 0.25 μ g/cm 2 surface area.

Techniques: Expressing, Quantitative RT-PCR, Immunostaining, Staining, Immunofluorescence, Labeling, Marker

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Phosphorylated PDGF receptors in proliferating hemangiomas. Immunostaining of proliferating hemangioma specimens for phospho-PDGFR- β ( a ) and phospho-PDGFR- α ( b ) (images taken at × 20, green=PDGFR, blue=DAPI)

Article Snippet: The cells were transfected with control shRNA (sc-108060; Santa Cruz Biotechnology), PDGFR- α shRNA (sc-29443-SH; Santa Cruz Biotechnology) plasmid DNA, or PDGFR- β shRNA (sc-29442-SH; Santa Cruz Biotechnology) at a concentration of 0.25 μ g/cm 2 surface area.

Techniques: Immunostaining

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Expression of PDGF signaling axis in hemangioma-derived CD133+ cells. ( a ) Expression of PDGFRs in hemSCs and bm-MPCs (data normalized to 18 S rRNA and presented as relative to bm-MPCs; two different hemSC preparations run in triplicates were averaged). ( b ) Expression of PDGF transcripts in hemSCs (data presented as in ( a ); * P <0.05 compared with bm-MPCs; right panel shows the expression of PDGF-A, PDGF-C, and PDGF-D at a lower scale). ( c ) PDGF-BB levels in media from cells cultured for 48 h in EBM2/20%FBS (with growth factors) (* P <0.05 compared with bm-MPCs). ( d ) PDGF-BB levels in cell lysates determined by ELISA (* P <0.05 compared with bm-MPCs). ( e ) Levels of phosphorylated PDGFR- α and - β in hemSCs with or without exogenous PDGF-BB stimulation (cells were stimulated with 10 ng/ml PDGF-BB; * P <0.05 compared with unstimulated cells). ( f ) Schematic illustration of the hypothesized PDGF polypeptides and the corresponding receptors on hemangioma stem cell surface

Article Snippet: The cells were transfected with control shRNA (sc-108060; Santa Cruz Biotechnology), PDGFR- α shRNA (sc-29443-SH; Santa Cruz Biotechnology) plasmid DNA, or PDGFR- β shRNA (sc-29442-SH; Santa Cruz Biotechnology) at a concentration of 0.25 μ g/cm 2 surface area.

Techniques: Expressing, Derivative Assay, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: PDGF-BB inhibits adipogenic differentiation in hemSCs. ( a ) Representative oil red O staining of hemSCs cultured in adipogenic differentiation media supplemented with 10 ng/ml of PDGF-AA, -AB, or -BB. ( b ) Quantitative analysis of adipogenic differentiation in the presence of PDGF polypeptides was assessed by real-time RT-PCR for adipogenesis-specific transcription factors, C/EBP α and PPAR γ (data normalized to 18 S rRNA and presented as relative to adipogenic media; * P <0.05 compared with the adipogenic media; n =3). ( c ) Effect of adipogenic differentiation on PDGFR expression in hemSCs at day 7. ( d ) Oil red O staining of hemSCs cultured with different growth factors (each at 10 ng/ml) illustrating specific inhibition of hemSC adipogenesis by PDGF signaling

Article Snippet: The cells were transfected with control shRNA (sc-108060; Santa Cruz Biotechnology), PDGFR- α shRNA (sc-29443-SH; Santa Cruz Biotechnology) plasmid DNA, or PDGFR- β shRNA (sc-29442-SH; Santa Cruz Biotechnology) at a concentration of 0.25 μ g/cm 2 surface area.

Techniques: Staining, Cell Culture, Quantitative RT-PCR, Expressing, Inhibition

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: Inhibiting cell-autogenous PDGF signaling enhances adipogenesis. ( a ) Oil red O staining of hemSCs exposed to adipogenic media with or without PDGFR inhibitors, AG-370 and AG-1296, and PDGFR neutralizing antibodies. ( b ) Quantitative analysis of C/EBP α and PPAR γ in cells exposed to PDGF inhibitors as in ( a ) (data presented relative to control media; * P <0.05 compared with control media; † P <0.05 compared with adipogenic media; n =3). ( c ) Effect of PDGFR- α and PDGFR- β- neutralizing antibodies and chemical inhibitors on reversing the inhibition by exogenous PDGF-BB (* P <0.05 compared with PDGF-BB treatment)

Article Snippet: The cells were transfected with control shRNA (sc-108060; Santa Cruz Biotechnology), PDGFR- α shRNA (sc-29443-SH; Santa Cruz Biotechnology) plasmid DNA, or PDGFR- β shRNA (sc-29442-SH; Santa Cruz Biotechnology) at a concentration of 0.25 μ g/cm 2 surface area.

Techniques: Staining, Control, Inhibition

Journal: Cell Death & Disease

Article Title: Intrinsic regulation of hemangioma involution by platelet-derived growth factor

doi: 10.1038/cddis.2012.58

Figure Lengend Snippet: PDGF-BB employs PDGFR- β in hemSCs. shRNA-mediated knockdown of PDGFR- α ( a ) and PDGFR- β ( b ) in hemSCs at sub-passage 1 (subP1; following 2 weeks of puromycin selection) and sub-passage 6 (six serial passages after puromycin selection) (* P <0.05 compared with control shRNA). ( c ) shRNA-knockdown of PDGFR- α and PDGFR- β in hemSCs reduced the inhibitory effect of PDGF-BB on hemSC adipogenesis (* P <0.05 compared with control shRNA). ( d ) Schematic illustrating the findings of the study and our working hypothesis. High levels of PDGF-BB from hemSCs during proliferation mediate intracellular signaling through PDGFR- β (predominantly PDGFR- ββ homodimer with contribution from PDGFR- αβ heterodimer) to inhibit C/EBP α and PPAR γ expression and adipogenesis (i.e., involution). Upon regression of the vessels and removal of the PDGF-BB source, adipogenesis is triggered

Article Snippet: The cells were transfected with control shRNA (sc-108060; Santa Cruz Biotechnology), PDGFR- α shRNA (sc-29443-SH; Santa Cruz Biotechnology) plasmid DNA, or PDGFR- β shRNA (sc-29442-SH; Santa Cruz Biotechnology) at a concentration of 0.25 μ g/cm 2 surface area.

Techniques: shRNA, Knockdown, Selection, Control, Expressing

Journal: Oncogenesis

Article Title: Platelet-derived growth factor receptor-α and -β promote cancer stem cell phenotypes in sarcomas

doi: 10.1038/s41389-018-0059-1

Figure Lengend Snippet: a Western blot analysis of phosphorylated and total PDGFR-α and -β in human sarcoma cell lines grown as monolayers and as spheroids. β-actin was used as a loading control. b Western blot analysis of PDGFR-α and -β expression and self-renewal transcription factors in human sarcoma cell lines grown as spheroids and treated with imatinib (1 μM) or DMSO control. Experiments in a , b were performed three times with similar results. c Graph displaying the mean diameters of the spheroids for HT1080, SK-LMS-1, and DDLS8817 cells treated with imatinib or DMSO. d Light microscopy images from single cell assays for spheroid cells treated with imatinib or DMSO. e Representative light microscopy images from spheroid formation assays performed in triplicate of human sarcoma cell lines under varying conditions. Cells were grown as spheroids following PDGFR-α and PDGFR-β double knockdown (sh.PDGFR-α/β), transduction with scramble shRNA (sh.Scr), or treatment with imatinib or DMSO. Graphs display the mean numbers of spheroids ≥50 μm per field in each treatment group. Bars represent standard deviation. * p < 0.05 compared to DMSO

Article Snippet: Silencing of PDGFR-α and PDGFR-β was achieved via lentiviral transduction of human PDGFR-α shRNA (sc-29443-V; Santa Cruz) and human PDGFR-β shRNA (sc-29442-V; Santa Cruz) per the manufacturer’s protocol.

Techniques: Western Blot, Control, Expressing, Light Microscopy, Knockdown, Transduction, shRNA, Standard Deviation

Journal: Oncogenesis

Article Title: Platelet-derived growth factor receptor-α and -β promote cancer stem cell phenotypes in sarcomas

doi: 10.1038/s41389-018-0059-1

Figure Lengend Snippet: a Tumor growth curves for HT1080 xenografts treated with doxorubicin (Dox) 4 mg/kg), imatinib (90 mg/kg), PDGFR-α shRNA and PDGFR-β shRNA (sh.PDGFR-α/β), and/or DMSO. There were five mice per group. b Representative immunofluorescence images of treated HT1080 tumors and stained for PCNA (purple), cleaved caspase-3 (red), and CD133 (green). White scale bars 25 μm; yellow scale bar 50 μm. c,d Graphs displaying the number of cleaved caspase 3( + ), CD133( + ), and dual cleaved caspase 3( + )/CD133( + ) cells on immunofluorescence imaging analysis. Bars represent standard deviation. * p < 0.05 compared to control and monotherapy groups

Article Snippet: Silencing of PDGFR-α and PDGFR-β was achieved via lentiviral transduction of human PDGFR-α shRNA (sc-29443-V; Santa Cruz) and human PDGFR-β shRNA (sc-29442-V; Santa Cruz) per the manufacturer’s protocol.

Techniques: shRNA, Immunofluorescence, Staining, Imaging, Standard Deviation, Control

Journal: Oncogenesis

Article Title: Platelet-derived growth factor receptor-α and -β promote cancer stem cell phenotypes in sarcomas

doi: 10.1038/s41389-018-0059-1

Figure Lengend Snippet: a Western blot analysis of HIF-1α, phosphorylated and total PDGFR-α and -β, and CD133 in human sarcoma cell lines grown as monolayers or as spheroids under normoxia (21% O 2 ) and hypoxia (1% O 2 ) for 18–24 h. b Graph displaying the mean numbers of spheroids >50 μm per field formed by sarcoma spheroid cells under normoxia and hypoxia. c Graphs displaying the mean numbers of spheroids >50 μm per field formed by sarcoma spheroid cells under normoxia and hypoxia following transduction with control shRNA (sh.Scr) or PDGFR-α and -β shRNA (sh.PDGFR-α/β). d Western blot analysis of HIF-1α, phosphorylated and total PDGFR-α and -β in human sarcoma spheroid cells under normoxia (21% O 2 ) and hypoxia (1% O 2 ) following transduction with control shRNA (sh.Scr) or HIF-1α shRNA (sh.HIF-1α). Experiments a and d were performed three times with similar results. Bars represent standard deviation. * p < 0.05 compared to Normoxia

Article Snippet: Silencing of PDGFR-α and PDGFR-β was achieved via lentiviral transduction of human PDGFR-α shRNA (sc-29443-V; Santa Cruz) and human PDGFR-β shRNA (sc-29442-V; Santa Cruz) per the manufacturer’s protocol.

Techniques: Western Blot, Transduction, Control, shRNA, Standard Deviation