Journal: PLoS ONE

Article Title: IFNγ-stimulated dendritic cell extracellular vesicles can be nasally administered to the brain and enter oligodendrocytes

doi: 10.1371/journal.pone.0255778

Figure Lengend Snippet: IFNγ-DC-EVs were transfected with reporter mCherry mRNA. (A) Nasal delivery of transfected EVs (NA mCherry-EV) confirmed EV delivery of functional mCherry mRNA to brain cells. Representative images show mCherry fluorescence (red), anti-mCherry immunostaining (green) and merged image (yellow) to confirm mCherry expression in cells. Animals that were nasally administered untransfected EVs (Sham) showed no mCherry expression/staining. Scale bar = 25 μm. (B) Representative merged images (B1-8) show widespread cellular distribution in the CNS two days after nasal administration of mCherry mRNA transfected IFNγ-DC-EVs. Images were taken from brain sections represented in the schematic. A subset of mCherry-positive cells colocalize with CNPase, indicating that they are oligodendrocytes. (C) Representative high power images of mCherry expression (red) in cells that co-stained (yellow) for CNPase (green). Scale bar = 25 μm. (D) Similarly, stained representative merged images (D1-8) show oligodendrocyte co-staining throughout the CNS, with numbers corresponding to brain areas in schematic (B). Scale bar = 20 μm. ( n = 5-7/group; A-D). To confirm that IFNγ-DC-EVs delivered cargo to oligodendrocytes, protein expression levels of known miR-219 mRNA targets expressed in oligodendrocyte lineage cells were quantified via immunoblot. Three days after nasal delivery of IFNγ-DC-EVs or Unstim-DC-EVs [Sham(S)], levels of (E) PDGFRα, and (F) ELOVL7 were significantly (* p = 0.040 and 0.038 respectively) reduced in the brains of animals that received IFNγ-DC-EVs. ( n = 3-6/group).

Article Snippet: Primary antibodies used were PDGFRα (#NBP2-67025; Novus Biologicals) at 1:5000, and ELOVL7 (#NBP1-93926; Novus Biologicals) at 1:750.

Techniques: Transfection, Functional Assay, Fluorescence, Immunostaining, Expressing, Staining, Western Blot

Journal: Neoplasia (New York, N.Y.)

Article Title: EGFRvIII-driven microenvironmental fibroblast activation and transformation accelerate oral cancer progression via lipocalin-2/STAT3 axis

doi: 10.1016/j.neo.2025.101193

Figure Lengend Snippet: Aberrant expression of EGFRvIII in OSCC. A. Schematic structure of EGFR WT and EGFRvIII. Representative agarose gel electrophoresis images show EGFRvIII-specific PCR bands, further confirmed by Sanger sequencing chromatograms and sequence alignments. A summary table indicates EGFRvIII positivity ("YES") or negativity ("NO") across OSCC specimens, with a mutation detection rate of approximately 70 %. B. Western blot analysis of EGFRvIII protein levels in OSCC cell lines. β-tubulin served as the loading control. Protein band intensities were quantified by densitometry, normalized to β-tubulin, and presented as numerical values below the gels. C. Quantitative real-time PCR (qRT-PCR) analysis of EGFRvIII mRNA expression in OSCC cell lines. GAPDH was used as the internal normalization control. D. Xenograft tumors were established by subcutaneous injection of MTCQ1 cells (mouse OSCC cells) overexpressing EGFRvIII or a control vector into C57BL/6 mice. Tumors were harvested and analyzed on day 35 post-injection. Representative photomicrographs and dot plots of immunohistochemical (IHC) staining demonstrate the expression of fibroblast activation markers (α-SMA, Collagen I, Vimentin, FAP, PDGFRA, and PDGFRB) in tumor tissues derived from MTCQ1-EGFRvIII and control vector groups. Scale bar: 300 μm; 40 × magnification. Data are presented as mean ± SD, and statistical significance was determined using Student’s t-test (* P < 0.05; * P < 0.01; N = 1).

Article Snippet: After dewaxing and rehydration, samples underwent antigen retrieval at pH6 (ER1)/pH9 (ER2) for 10–30 min, and the diluent was used for protein blocking. α‐SMA Ab (ER2 30 min, 1 : 200, catalog no NB600‐531), PDGFRA Ab (ER2 20 min 1 : 200, catalog no NBP2-67025; Novus Biologicals, Abingdon, Oxon, UK), PDGFRB Ab (ER2 20 min, 1 : 200, catalog no ab32570), Vimentin (ER1 20 min 1 :200, catalog no ab92547; Abcam), Collagen I Ab (ER1 20 min 1 :100, catalog no IR292-962; iReal) were detected with the Bond Polymer Refine Detection (Leica Microsystems), and FAP Ab (ER1 20 min 1 : 100, catalog no ab207178; Abcam), followed by counterstaining with hematoxylin and bluing reagent (Leica Microsystems).

Techniques: Expressing, Agarose Gel Electrophoresis, Sequencing, Mutagenesis, Western Blot, Control, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Injection, Plasmid Preparation, Immunohistochemical staining, Immunohistochemistry, Activation Assay, Derivative Assay

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 12 CD34/PDGFR-α levels in studied groups. (*: significantly different from the control group. CTRL control group, ET endurance training group, RT: resistance training group)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Control

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 13 Changes in telocytes number in response to exercise. Double-immunofluorescence labeling for CD34/PDGFR-α used to determine telocytes: these cells are increased in response to 8 weeks of endurance training (ET) and resistance training (RT)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Immunofluorescence, Labeling

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 15 Changes in telocytes number in response to exercise. Double-immunofluorescence labeling for CD34/PDGFR-β used to determine telocytes: these cells are increased in response to 8 weeks of endurance training (ET) and resistance training (RT)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Immunofluorescence, Labeling

Journal: The journal of physiological sciences : JPS

Article Title: Telocytes response to cardiac growth induced by resistance exercise training and endurance exercise training in adult male rats.

doi: 10.1186/s12576-023-00868-2

Figure Lengend Snippet: Fig. 14 CD34/PDGFR-β levels in studied groups. (*: significantly different from the control group. CTRL control group, ET endurance training group, RT resistance training group)

Article Snippet: The rabbit polyclonal antibody to PDGFR-α (Biorbyt, UK), rabbit polyclonal antibody to PDGFR-β (Biorbyt, UK) and rabbit polyclonal antibody to CD34 (Biorbyt, UK) were added to the sample and incubated in a refrigerator at 2–8 °C for overnight in a humid environment to prevent the tissue from drying out.

Techniques: Control