Journal: Journal of Functional Foods

Article Title: The skin hydration and anti-inflammatory potential of zerumbone, a natural sesquiterpene of Zingiber zerumbet, enhanced Src/ERK-mediated HAS-2/AQP-3 and inhibited NFκB/AP-1 expression in UVB-irradiated human keratinocytes

doi: 10.1016/j.jff.2023.105890

Figure Lengend Snippet: Fig. 5. Src, ERK, and CREB signaling pathway were upregulated by Zr treatment in HaCaT cells. A-B, p-Src and Src protein expression was determined with western blotting. Cells were treated with Zer (10 μM) for 0–240 min or Zer (0–10 μM) for 30 min. C, p-ERK and ERK expression was evaluated using western blot. Cells were treated with Zer (0–10 μM) for 60 min. D-E, p-CREB and CREB expressions were determined using western blot. Cells were treated with Zer (10 μM) for 0–120 min or Zer (0–10 μM) for 60 min. F-G, Zer has no effect on STAT3 and AKT/NFκB/IκB signaling pathways in HaCaT cells. F, p-STAT3, STAT3, and NFκB expression was determined using western blot. Cells were treated with Zer (10 μM) for 120 min. G, p-STAT3, p-AKT, AKT, and p-IκBα expression was determined with western blot. Cells were treated with Zer (0–10 μM) for 60 min.

Article Snippet: Antibodies against Nrf2, Keap-1, β-actin, iNOS, COX-2, IKKα, HAS-2, CREB (cAMP response element binding protein), p-CREB, IκBα, p-NFκB, Plateletderived growth factor A (PDGF-A), Vascular endothelial growth factor (VEGF), and β-tubulin were obtained from Santa Cruz Biotechnology Inc. (Heidelberg, Germany).

Techniques: Expressing, Western Blot, Protein-Protein interactions

Journal: Journal of Functional Foods

Article Title: The skin hydration and anti-inflammatory potential of zerumbone, a natural sesquiterpene of Zingiber zerumbet, enhanced Src/ERK-mediated HAS-2/AQP-3 and inhibited NFκB/AP-1 expression in UVB-irradiated human keratinocytes

doi: 10.1016/j.jff.2023.105890

Figure Lengend Snippet: Fig. 7. Zer suppresses UVB-activated NFκB through IKK-mediated IκB degradation in HaCaT cells. Cells underwent a 24 h pretreatment with Zer (0–10 μM) followed by with or without UVB (30 mJ/cm2). A, Western blot analysis showing the protein levels of p-NFκB (p65), NFκB (p65), IκBα, p-IκBα, and p-IKK. B-E, The intracellular p-NFκB (p65) and NFκB (p65) levels as shown with immunofluorescence labeling. Using a confocal microscope, the subcellular localization of p-NFκB (p65) and NFκB (p65) was investigated. Results are the mean ± SD (n = 3). **p < 0.01; ***p < 0.001 compared to untreated cells. ###p < 0.001 compared to UVB-irradiated cells.

Article Snippet: Antibodies against Nrf2, Keap-1, β-actin, iNOS, COX-2, IKKα, HAS-2, CREB (cAMP response element binding protein), p-CREB, IκBα, p-NFκB, Plateletderived growth factor A (PDGF-A), Vascular endothelial growth factor (VEGF), and β-tubulin were obtained from Santa Cruz Biotechnology Inc. (Heidelberg, Germany).

Techniques: Western Blot, Immunofluorescence, Labeling, Microscopy, Irradiation

Journal: Materials Today Bio

Article Title: 3D-printed PRP-infused double-network hydrogels orchestrate inflammation resolution and vascular regeneration in infected wounds

doi: 10.1016/j.mtbio.2026.102841

Figure Lengend Snippet: In vitro Anti-inflammatory ability of hydrogels by driving macrophage polarization. (A) Immunofluorescence images of the M1 and M2-type macrophages in different groups. (B, C) Quantitative analysis of positive cells (CD86 and CD206) fluorescence intensity values. (D) Western blot analysis for the expression of inflammation proteins (Arg-1 and iNOS) in RAW264.7 with hydrogels treatments, and (E, F) Semiquantitative analysis of corresponding protein expression levels. (G–I) ELISA analysis of specific markers of M1 macrophage and M2 macrophage with various treatments. (mean ± SD, n = 3, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001).

Article Snippet: The amount of growth factors released from the hydrogel was quantified using the QuantiCyto® Rat EGF ELISA Kit (Neobioscience Technology Co, Ltd.), Rat PDGF-A ELISA kit, and Rat VEGF ELISA Kit (CUSABIO, https://www.cusabio.com/ ).

Techniques: In Vitro, Immunofluorescence, Fluorescence, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay