pdest27 Search Results


n a gfp a catenin addgene 20139 gfp myoii addgene 11347 gfp zo1 addgene 30313 ezrin gfp addgene 20680 ezrin mruby2  (Addgene inc)
93
Addgene inc n a gfp a catenin addgene 20139 gfp myoii addgene 11347 gfp zo1 addgene 30313 ezrin gfp addgene 20680 ezrin mruby2
N A Gfp A Catenin Addgene 20139 Gfp Myoii Addgene 11347 Gfp Zo1 Addgene 30313 Ezrin Gfp Addgene 20680 Ezrin Mruby2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/pm29576449-251-131-133?v=Addgene+inc
Average 93 stars, based on 1 article reviews
n a gfp a catenin addgene 20139 gfp myoii addgene 11347 gfp zo1 addgene 30313 ezrin gfp addgene 20680 ezrin mruby2 - by Bioz Stars, 2026-08
93/100 stars
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aa 217 548 cc1  (Addgene inc)
93
Addgene inc aa 217 548 cc1
Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
Aa 217 548 Cc1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/pmc12516129-254-4-12?v=Addgene+inc
Average 93 stars, based on 1 article reviews
aa 217 548 cc1 - by Bioz Stars, 2026-08
93/100 stars
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pbad18 kan  (Addgene inc)
90
Addgene inc pbad18 kan
Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
Pbad18 Kan, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/pmc08440630-262-13-18?v=Addgene+inc
Average 90 stars, based on 1 article reviews
pbad18 kan - by Bioz Stars, 2026-08
90/100 stars
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pdest27 vinculin  (Addgene inc)
93
Addgene inc pdest27 vinculin
Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
Pdest27 Vinculin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/pmc09984485-53-0-2?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pdest27 vinculin - by Bioz Stars, 2026-08
93/100 stars
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pdest27 gst atxn1 fl 30q  (Addgene inc)
93
Addgene inc pdest27 gst atxn1 fl 30q
Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
Pdest27 Gst Atxn1 Fl 30q, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/pmc09700751-49-13-21?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pdest27 gst atxn1 fl 30q - by Bioz Stars, 2026-08
93/100 stars
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pdest27  (Addgene inc)
93
Addgene inc pdest27
Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
Pdest27, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/pmc03226282-55-26-5?v=Addgene+inc
Average 93 stars, based on 1 article reviews
pdest27 - by Bioz Stars, 2026-08
93/100 stars
  Buy from Supplier
plasmid pdest27-naa25  (imaGenes GmbH)
90
imaGenes GmbH plasmid pdest27-naa25
Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
Plasmid Pdest27 Naa25, supplied by imaGenes GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/10__1186_slash_1753___6561___3___s6___s4-38-1-6?v=imaGenes+GmbH
Average 90 stars, based on 1 article reviews
plasmid pdest27-naa25 - by Bioz Stars, 2026-08
90/100 stars
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pdest27-mrckβ_cat  (Promega)
90
Promega pdest27-mrckβ_cat
Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
Pdest27 Mrckβ Cat, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pdest27/pmc04121984-265-2-12?v=Promega
Average 90 stars, based on 1 article reviews
pdest27-mrckβ_cat - by Bioz Stars, 2026-08
90/100 stars
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pdest27-mbin1+13+17 (plasmid #61097)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pdest27-mbin1+13 (plasmid #61087)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pdest27-mbin1+17 (plasmid #61096)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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pdest27-wfs1 (plasmid #79276)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor GST–p150-CC1. n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.

Journal: Journal of Cell Science

Article Title: An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts

doi: 10.1242/jcs.263766

Figure Lengend Snippet: Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor GST–p150-CC1. n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.

Article Snippet: The chicken DCTN1 p150Glued AA 217–548 (CC1) was from the plasmid pVEX-CC1 (Addgene plasmid 74170; http://n2t.net/addgene:74170 ; RRID:Addgene_74170; deposited by Trina Schroer).

Techniques: Activity Assay, Control, Imaging

Noncanonical asters can merge. (A) Confocal time-lapse montage of microtubule and EB1–GFP dynamics in egg extracts, showing that the centers of two noncanonical asters merged with each another spontaneously, and that the EB1–GFP-enriched regions at the centers also merged. Each image is a maximum-intensity projection of four confocal planes spanning 6 µm of depth. Imaging started at an arbitrary time point after the asters had formed but had not merged. The plot below each image is the fluorescence intensity profile along a 1.65 µm thick, 22.8 µm long line segment (yellow dashed rectangle) that starts at the bottom left and ends at the top right. For each point on the curve in the plot, the horizontal coordinate is the distance from the start of the line segment, and the vertical coordinate is the average fluorescence intensity of the pixels across the width of the line segment at that distance (a.u., arbitrary units). The black arrows indicate intensity peaks for microtubule (second row) and EB1–GFP (fourth row) fluorescence at the aster centers. n =9. (B) Confocal images of microtubules in control and GST–p150-CC1-treated extracts, showing that noncanonical asters still merged when dynein was inhibited by 2 µM GST–p150-CC1. n =2.

Journal: Journal of Cell Science

Article Title: An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts

doi: 10.1242/jcs.263766

Figure Lengend Snippet: Noncanonical asters can merge. (A) Confocal time-lapse montage of microtubule and EB1–GFP dynamics in egg extracts, showing that the centers of two noncanonical asters merged with each another spontaneously, and that the EB1–GFP-enriched regions at the centers also merged. Each image is a maximum-intensity projection of four confocal planes spanning 6 µm of depth. Imaging started at an arbitrary time point after the asters had formed but had not merged. The plot below each image is the fluorescence intensity profile along a 1.65 µm thick, 22.8 µm long line segment (yellow dashed rectangle) that starts at the bottom left and ends at the top right. For each point on the curve in the plot, the horizontal coordinate is the distance from the start of the line segment, and the vertical coordinate is the average fluorescence intensity of the pixels across the width of the line segment at that distance (a.u., arbitrary units). The black arrows indicate intensity peaks for microtubule (second row) and EB1–GFP (fourth row) fluorescence at the aster centers. n =9. (B) Confocal images of microtubules in control and GST–p150-CC1-treated extracts, showing that noncanonical asters still merged when dynein was inhibited by 2 µM GST–p150-CC1. n =2.

Article Snippet: The chicken DCTN1 p150Glued AA 217–548 (CC1) was from the plasmid pVEX-CC1 (Addgene plasmid 74170; http://n2t.net/addgene:74170 ; RRID:Addgene_74170; deposited by Trina Schroer).

Techniques: Imaging, Fluorescence, Control