Journal: The Journal of Biological Chemistry

Article Title: Upregulation of CYR61 by TGF-β and YAP signaling exerts a counter-suppression of hepatocellular carcinoma

doi: 10.1016/j.jbc.2024.107208

Figure Lengend Snippet: TGF-β and Hippo signaling coordinately regulate CYR61 gene transcription. A and B , HCCLM3 or HLE cells were treated with 2.5 ng/ml of TGF-β1 in the presence or absence of small chemical inhibitors of different signaling pathways for 24 h, followed by gene expression analyses by quantitative PCR ( A ) or Western blotting ( B ). TGF-β type I receptor inhibitor, SB431542 (5 μM); YAP inhibitor, verteporfin (5 μM); MEK inhibitor, PD0325901 (5 μM); JNK inhibitor, SP600125 (5 μM); GSK-3 inhibitor, SB216763 (5 μM); NF-κB inhibitor, JSH-23 (5 μM); PI3K inhibitor, LY294002 (5 μM); ERK1/2 inhibitor, LY3214996 (5 μM). C , HLE or HCCLM3 cells treated with 2.5 ng/ml of TGF-β1 and/or 3 μM of XMU-MP-1 for 24 h were harvested for protein expression detection by Western blotting. D and E , HLE or HCCLM3 cells were treated with 2.5 ng/ml of TGF-β1 ( D ) or 3 μM of XMU-MP-1 ( E ) for 24 h, in the presence or absence of 5 μM of SB431542 and/or 5 μM of verteporfin. Then cells were analyzed by Western blotting. F and G , liver cancer cells transfected with siRNAs were treated with or without 2.5 ng/ml of TGF-β1 ( F ) or 3 μM of XMU-MP-1 ( G ) for 24 h before being harvested for Western blotting analyses. TGF-β, transforming growth factor-β.

Article Snippet: Small chemical inhibitors, such as JSH-23 (HY-13982), SB216763 (HY-12012), verteporfin (HY-B0146), SB431542 (HY-10431), PD0325901 (HY-131295), SP600125 (HY-12041), LY3214996 (HY-101494), and LY294002 (HY-10108), were all purchased from MedChemExpress (MCE), while XMU-MP-1 was obtained from AbMole (M9057).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Transfection