Journal: Infection and Immunity

Article Title: Characterization and Evaluation of the Moraxella catarrhalis Oligopeptide Permease A as a Mucosal Vaccine Antigen

doi: 10.1128/iai.00314-10

Figure Lengend Snippet: FIG. 1. Characterization of the oppA gene. (A) Schematic illustration of oppA with the other oligopeptide transporter genes in the genomes of M. catarrhalis ATCC 43617 and S. pyogenes. Numbers indicate the initial and terminal nucleotide positions of each gene within the GenBank sequence. (B) Ethidium bromide-stained agarose gel showing amplicons of the oppA gene amplified from 20 clinical isolates of M. catarrhalis and the ATCC 43617 strain. Lanes 1 and 23, DNA standards; lanes 2 to 11, amplicons from middle ear fluid isolates 135, 238, 555, 2901, 3584, 3614, 5488, 6952, 8184, and 9483, respectively; lane 12, amplicon from ATCC 43617; lanes 13 to 22, amplicons from sputum isolates 5P34B1, 10P58B2, 12P15B1, 14P25B1, 29P24B1, 33P25B2, 39P29B2, 55P18B3, 63P62B1, and 96P9B1, respectively. The sizes of the DNA standards (in kilobases) are indicated on the right. (C) Results of reverse transcriptase PCR (RT-PCR) with RNA extracted from M. catarrhalis 035E to detect oppA transcript. Lane 1, DNA standards; lane 2, RT-PCR product; lane 3, RT-PCR in the absence of reverse transcriptase; lane 4, PCR product from genomic DNA template. DNA standards are noted in base pairs on the left.

Article Snippet: The results showed that the DNA sequence of the PCR product from ATCC 43617 was identical to the oppA gene sequence in GenBank, which validated the sequence information derived from our PCR products.

Techniques: Sequencing, Staining, Agarose Gel Electrophoresis, Amplification, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction

Journal: Infection and Immunity

Article Title: Characterization and Evaluation of the Moraxella catarrhalis Oligopeptide Permease A as a Mucosal Vaccine Antigen

doi: 10.1128/iai.00314-10

Figure Lengend Snippet: FIG. 4. Construction and characterization of the isogenic oppA mutant M. catarrhalis 035E strain. The oppA gene (from nt 97 to 1900) was replaced by the kanamycin resistance gene via homologous recom- bination in the oppA mutant. (A) Schematic depiction of the trans- forming DNA fragment in relation to the oppA gene in the genome. Numbers under the line indicate the nucleotide position within the oppA gene. USG, upstream gene; DSG, downstream gene. (B) Paired PCRs to examine the targeted mutagenesis in the oppA mutant com- pared to the wild type. (Left panel) Lane 1, DNA standards; lanes 2 and 3, PCR of the F1 fragment; lanes 4 and 5, PCR of the F2 fragment; lanes 6 and 7, PCR of a region (nt 1073 to 1811) of the oppA gene; lanes 8 and 9, PCR of the F3 fragment; lanes 2, 4, 6, and 8, PCR with the wild-type DNA as template; lanes 3, 5, 7, and 9, PCR with the oppA mutant DNA as template. (Right panel) PCR of the whole region of genes involved in the mutagenesis with the forward primer of F1 and the reverse primer of F3; lane 1, PCR with the wild-type DNA as template; lane 2, PCR with the mutant DNA as template; lane 3, DNA standards noted in kilobases. (C and D) Immunoblot assays of whole- cell lysates of the wild-type M. catarrhalis 035E and the oppA mutant. (C) Immunoblot with rabbit anti-OppA antibody. (D) Immunoblot with rabbit anti-CD antibody. Lanes 1, protein standards (kilodaltons); lanes 2, whole-cell lysate of the oppA mutant; lanes 3, whole-cell lysate of wild-type 035E. (E) Immunoblot assay with rabbit antiserum raised to whole cells of M. catarrhalis 035E. Lane 1, whole-cell lysate of the wild type; lane 2, whole-cell lysate of the oppA mutant. The arrow denotes OppA protein in the wild-type strain.

Article Snippet: The results showed that the DNA sequence of the PCR product from ATCC 43617 was identical to the oppA gene sequence in GenBank, which validated the sequence information derived from our PCR products.

Techniques: Mutagenesis, Western Blot

Journal: BMC Medical Genomics

Article Title: Association between OX40L polymorphism and type 2 diabetes mellitus in Iranians

doi: 10.1186/s12920-024-01958-9

Figure Lengend Snippet: Genotype and allele distribution of OX40L gene in the control and T2DM subjects

Article Snippet: The rs10912580 PCR product (372 bp) was digested by AccI (Fermentas Company, 10 U, 16 h overnight) to detect G/G (199 and 173 bp), A/G (199,173 and 372 bp) and A/A (372 bp) genotypes.

Techniques: Control

Journal: BMC Medical Genomics

Article Title: Association between OX40L polymorphism and type 2 diabetes mellitus in Iranians

doi: 10.1186/s12920-024-01958-9

Figure Lengend Snippet: rs10912580 association with response status ( n = 368, crude analysis)

Article Snippet: The rs10912580 PCR product (372 bp) was digested by AccI (Fermentas Company, 10 U, 16 h overnight) to detect G/G (199 and 173 bp), A/G (199,173 and 372 bp) and A/A (372 bp) genotypes.

Techniques: Control