Journal: The American Journal of Pathology

Article Title: TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma

doi: 10.1016/j.ajpath.2019.01.016

Figure Lengend Snippet: Primary Antibodies Used for IHC and WB

Article Snippet: Transient transfection experiments with TEAD2VP16 and TEAD4 (in pCMV6-Neo plasmid; Origene Technologies Inc., Rockville, MD; number SC111037) constructs were conducted in HepG2 and Hep293TT cell lines using the Lipofectamine 2000 Reagent (Thermo Fisher Scientific).

Techniques:

Journal: The American Journal of Pathology

Article Title: TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma

doi: 10.1016/j.ajpath.2019.01.016

Figure Lengend Snippet: Real-Time Quantitative RT-PCR Primer Sequence Information

Article Snippet: Transient transfection experiments with TEAD2VP16 and TEAD4 (in pCMV6-Neo plasmid; Origene Technologies Inc., Rockville, MD; number SC111037) constructs were conducted in HepG2 and Hep293TT cell lines using the Lipofectamine 2000 Reagent (Thermo Fisher Scientific).

Techniques: Quantitative RT-PCR, Sequencing

Journal: The American Journal of Pathology

Article Title: TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma

doi: 10.1016/j.ajpath.2019.01.016

Figure Lengend Snippet: TEAD4 is the predominant TEAD isoform in human hepatoblastoma (HB) samples. Real-time quantitative RT-PCR of TEAD1, TEAD2, TEAD3, and TEAD4 mRNA levels in 14 paired human normal liver and corresponding HB samples. Each symbol represents the mean value of an individual sample. ∗∗P < 0.01, ∗∗∗P < 0.001 when compared with normal liver (U-test). NT, nontumorous liver; T, tumor.

Article Snippet: Transient transfection experiments with TEAD2VP16 and TEAD4 (in pCMV6-Neo plasmid; Origene Technologies Inc., Rockville, MD; number SC111037) constructs were conducted in HepG2 and Hep293TT cell lines using the Lipofectamine 2000 Reagent (Thermo Fisher Scientific).

Techniques: Quantitative RT-PCR

Journal: The American Journal of Pathology

Article Title: TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma

doi: 10.1016/j.ajpath.2019.01.016

Figure Lengend Snippet: Frequent activation of TEAD4 in human hepatoblastoma (HB) specimens. Immunohistochemical pattern of TEAD4 protein in normal liver (A) and two human HB specimens (B). Normal liver exhibits faint/absent cytoplasmic immunoreactivity for Tead4, whereas HB lesions display strong nuclear accumulation of the protein. The three specimens are depicted in two magnifications. Scale bars: 500 μm (A and B, top row); 100 μm (A and B, bottom row). Original magnifications: ×40 (A and B, top row); ×200 (A and B, bottom row). H&E, hematoxylin and eosin.

Article Snippet: Transient transfection experiments with TEAD2VP16 and TEAD4 (in pCMV6-Neo plasmid; Origene Technologies Inc., Rockville, MD; number SC111037) constructs were conducted in HepG2 and Hep293TT cell lines using the Lipofectamine 2000 Reagent (Thermo Fisher Scientific).

Techniques: Activation Assay, Immunohistochemical staining

Journal: The American Journal of Pathology

Article Title: TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma

doi: 10.1016/j.ajpath.2019.01.016

Figure Lengend Snippet: Silencing of TEAD4 inhibits Yap target gene expression in hepatoblastoma (HB) cell lines. HepG2 (A) or Hep293TT (B) human HB cell lines were transfected with scrambled siRNA (siSC), small interfering (si) TEAD1, siTEAD2, siTEAD3, or siTEAD4. The expression of TEAD isoforms as well as that of YAP/TEAD target genes CTGF, CYR61, AXL, and SURVIVIN were analyzed using real-time quantitative RT-PCR. Data are expressed as means ± SD (A and B). n = 3 independent replicates (A and B). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 when compared with siSC (U-test).

Article Snippet: Transient transfection experiments with TEAD2VP16 and TEAD4 (in pCMV6-Neo plasmid; Origene Technologies Inc., Rockville, MD; number SC111037) constructs were conducted in HepG2 and Hep293TT cell lines using the Lipofectamine 2000 Reagent (Thermo Fisher Scientific).

Techniques: Expressing, Transfection, Quantitative RT-PCR

Journal: The American Journal of Pathology

Article Title: TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma

doi: 10.1016/j.ajpath.2019.01.016

Figure Lengend Snippet: Silencing of TEAD4 decreases proliferation and augments apoptosis in hepatoblastoma (HB) cell lines. HepG2 (A) and Hep293TT (B) human HB cell lines were transfected with scrambled siRNA (siSC), small interfering (si) TEAD1, siTEAD2, siTEAD3, or siTEAD4. Cell proliferation and apoptosis were measured. Untreated cells cultured in serum-free medium for 24 hours were used as baseline (ie, 1), and all cell proliferation and apoptosis rates were normalized to the baseline values. Data are expressed as means ± SD (A and B). n = 5 independent replicates (A and B). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 when compared with siSC (U-test).

Article Snippet: Transient transfection experiments with TEAD2VP16 and TEAD4 (in pCMV6-Neo plasmid; Origene Technologies Inc., Rockville, MD; number SC111037) constructs were conducted in HepG2 and Hep293TT cell lines using the Lipofectamine 2000 Reagent (Thermo Fisher Scientific).

Techniques: Transfection, Cell Culture

Journal: The American Journal of Pathology

Article Title: TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma

doi: 10.1016/j.ajpath.2019.01.016

Figure Lengend Snippet: Overexpression of TEAD4 increases proliferation and augments apoptosis in hepatoblastoma (HB) cell lines. HepG2 (A) or Hep293TT (B) human HB cell lines were transfected with empty vector or TEAD4. Cell proliferation and apoptosis were measured. Untreated cells cultured in serum-free medium for 24 hours were used as baseline (ie, 1), and all cell proliferation and apoptosis rates were normalized to the baseline values. Data are expressed as means ± SD (A and B). n = 5 independent replicates (A and B). ∗∗∗P < 0.001 when compared with empty vector (U-test).

Article Snippet: Transient transfection experiments with TEAD2VP16 and TEAD4 (in pCMV6-Neo plasmid; Origene Technologies Inc., Rockville, MD; number SC111037) constructs were conducted in HepG2 and Hep293TT cell lines using the Lipofectamine 2000 Reagent (Thermo Fisher Scientific).

Techniques: Over Expression, Transfection, Plasmid Preparation, Cell Culture

Journal: iScience

Article Title: A defective mechanosensing pathway affects fibroblast-to-myofibroblast transition in the old male mouse heart

doi: 10.1016/j.isci.2023.107283

Figure Lengend Snippet:

Article Snippet: Control cells were transfected with 2 μg of PCMV6-Kan/ Neo Untagged Cloning Vector (Origene #PCMV6KN), following the same protocol.

Techniques: Recombinant, Transfection, Blocking Assay, Staining, Protease Inhibitor, BIA-KA, Protein Concentration, Mass Spectrometry, Negative Control, Plasmid Preparation, Clone Assay, Software