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Journal: Cell reports

Article Title: A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit

doi: 10.1016/j.celrep.2023.113260

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Lentivirus was made for pLV-EF1a-SNAP-FRB-FIS1(MTS), pLv-EF1a-FKBP-PINK1(110–581)-mNeonGreen, pLv-EF1a-Parkin-A92mKO2, and pLv-EF1a-Parkin-A92mKO2 mutants, using HEK293T cells, pMD2.G (Addgene, 12259), pCMV-dR8.91 (NovoPro, V007548), and a lipofectamine 3000 kit (Invitrogen, L3000–015).

Techniques: Recombinant, Bradford Assay, Mutagenesis, Plasmid Preparation, Microscopy, Cell Culture, Software, Sequencing