pcbasce i-scei expression vector Search Results


scei expression plasmid  (Addgene inc)
93
Addgene inc scei expression plasmid
Scei Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/10__7554_slash_elife__91611-405-6-10?v=Addgene+inc
Average 93 stars, based on 1 article reviews
scei expression plasmid - by Bioz Stars, 2026-08
93/100 stars
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i-scei expression plasmid pcbasce  (Promega)
90
Promega i-scei expression plasmid pcbasce
I Scei Expression Plasmid Pcbasce, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/pmc04490184-360-17-32?v=Promega
Average 90 stars, based on 1 article reviews
i-scei expression plasmid pcbasce - by Bioz Stars, 2026-08
90/100 stars
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i scei expression vector pcbasce  (Addgene inc)
96
Addgene inc i scei expression vector pcbasce
I Scei Expression Vector Pcbasce, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/pmc12256224-65-13-17?v=Addgene+inc
Average 96 stars, based on 1 article reviews
i scei expression vector pcbasce - by Bioz Stars, 2026-08
96/100 stars
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i scei pcbasce  (Addgene inc)
92
Addgene inc i scei pcbasce
I Scei Pcbasce, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/pmc07264468-407-7-14?v=Addgene+inc
Average 92 stars, based on 1 article reviews
i scei pcbasce - by Bioz Stars, 2026-08
92/100 stars
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i scei site  (New England Biolabs)
97
New England Biolabs i scei site
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
I Scei Site, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/pmc00534631-72-57-12?v=New+England+Biolabs
Average 97 stars, based on 1 article reviews
i scei site - by Bioz Stars, 2026-08
97/100 stars
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cas9 sgrna expression vectors  (Addgene inc)
96
Addgene inc cas9 sgrna expression vectors
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
Cas9 Sgrna Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/us12371709-430-43-47?v=Addgene+inc
Average 96 stars, based on 1 article reviews
cas9 sgrna expression vectors - by Bioz Stars, 2026-08
96/100 stars
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non similar plasmid  (Addgene inc)
93
Addgene inc non similar plasmid
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
Non Similar Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/us12371709-430-32-35?v=Addgene+inc
Average 93 stars, based on 1 article reviews
non similar plasmid - by Bioz Stars, 2026-08
93/100 stars
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facscan  (Becton Dickinson)
90
Becton Dickinson facscan
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
Facscan, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/pmc03261725-346-41-45?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
facscan - by Bioz Stars, 2026-08
90/100 stars
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nucleofector solution v  (Lonza)
90
Lonza nucleofector solution v
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
Nucleofector Solution V, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/pmc05007816-56-28-31?v=Lonza
Average 90 stars, based on 1 article reviews
nucleofector solution v - by Bioz Stars, 2026-08
90/100 stars
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fugene 6  (Promega)
90
Promega fugene 6
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
Fugene 6, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/10__1038_slash_nchembio__1455-647-17-21?v=Promega
Average 90 stars, based on 1 article reviews
fugene 6 - by Bioz Stars, 2026-08
90/100 stars
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cuvette  (Bio-Rad)
96
Bio-Rad cuvette
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/bio_rxiv__2020__11__16__384677-350-22-23?v=Bio-Rad
Average 96 stars, based on 1 article reviews
cuvette - by Bioz Stars, 2026-08
96/100 stars
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transfection reagent fugene 6  (Promega)
90
Promega transfection reagent fugene 6
Possible repair products resulting <t>from</t> <t>DSB</t> induction at the <t>I-SceI</t> site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).
Transfection Reagent Fugene 6, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcbasce+i-scei+expression+vector/pm24531842-647-17-21?v=Promega
Average 90 stars, based on 1 article reviews
transfection reagent fugene 6 - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Possible repair products resulting from DSB induction at the I-SceI site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).

Journal:

Article Title: Preferential accessibility to specific genomic loci for the repair of double-strand breaks in human cells

doi: 10.1093/nar/gkh952

Figure Lengend Snippet: Possible repair products resulting from DSB induction at the I-SceI site of the integrated pL1HsSceI vector. (A) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo). The 2.5 kb L1 portion corresponds to the 3′ segment (3390–5976 bp) of cD11 (cDNA of an active L1 element), inside which the 23 bp NheI–BamHI segment was replaced by an 18 bp I-SceI restriction site (black box). (B) Structure of a typical NHEJ repair product, characterized by the retention of the Sp (SpeI) and S (SacII) plasmidic sites, the loss of the I-SceI site and the absence of the N B (NheI–BamHI) segment. NHEJ junctions frequently showed deletion and/or insertion sequences at the break site. (C) Structure of a typical NHCR repair product, characterized by the loss of the SpeI and SacII plasmidic sites and the absence of the NheI–BamHI segment and the acquisition of a novel chromosomal junction (dotted line). (D) Structure of a typical gene conversion product, characterized by the retention of the SpeI and SacII plasmidic sites and the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR. (E) Structure of a typical crossing-over/one sided invasion product, characterized by the presence of the NheI–BamHI segment acquired from an endogenous L1 partner by HR but extending beyond the homologous L1 sequences into adjacent chromosomal sequences (dotted line).

Article Snippet: PCBASceTcr expression vector was created by cloning the PCR-amplified pBR322 tetracycline-resistance gene (New England Biolabs) in the ScaI site of pCBASce ( 17 ), in order to inactivate the ampicillin-resistance gene. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Possible repair products resulting from DSB induction at the I-SceI site of the integrated pL1HsSceI vector. ( A ) Structure of the integrated pL1HsSceI vector containing origin of replication (ori), ampicillin gene resistance (amp) and neomycin gene selection (neo).

Techniques: Plasmid Preparation, Selection