Journal: Behavioral and Brain Functions : BBF

Article Title: Long-term effects of environmentally relevant doses of 2,2',4,4',5,5' hexachlorobiphenyl (PCB153) on neurobehavioural development, health and spontaneous behaviour in maternally exposed mice

doi: 10.1186/1744-9081-7-3

Figure Lengend Snippet: Feed concentration, body weight, reproductive output and feed intake by reproducing dams fed PCB153 in a fish or a casein-based diet.

Article Snippet: To mimic human exposure animals were exposed to PCB153 via ad libitum food intake, through diets spiked with PCB153 (Chiron AS, Norway).

Techniques: Concentration Assay

Journal: Behavioral and Brain Functions : BBF

Article Title: Long-term effects of environmentally relevant doses of 2,2',4,4',5,5' hexachlorobiphenyl (PCB153) on neurobehavioural development, health and spontaneous behaviour in maternally exposed mice

doi: 10.1186/1744-9081-7-3

Figure Lengend Snippet: Body weight gain in juvenile mice week 4 to 16 . Each point with error bars represents the mean body weight ± SEM in female (left panel) and male (right panel) BALB/c mice exposed to PCB153 during gestation and lactation. The data include animals that survived until week16.

Article Snippet: To mimic human exposure animals were exposed to PCB153 via ad libitum food intake, through diets spiked with PCB153 (Chiron AS, Norway).

Techniques:

Journal: Behavioral and Brain Functions : BBF

Article Title: Long-term effects of environmentally relevant doses of 2,2',4,4',5,5' hexachlorobiphenyl (PCB153) on neurobehavioural development, health and spontaneous behaviour in maternally exposed mice

doi: 10.1186/1744-9081-7-3

Figure Lengend Snippet: Thyroid hormones in serum at week16 in murine offspring . Hormone concentrations of free triiodothyronine (fT3, upper panel), free thyroxine (fT4, middle panel) and the ratio fT4/fT3 (lower panel) in pups exposed to PCB153 via gestation and lactation. The boxes show the median and the inter-quartile range. * indicates significant difference between genders (Mann-Whitney U pair wise comparison, p < 0.05).

Article Snippet: To mimic human exposure animals were exposed to PCB153 via ad libitum food intake, through diets spiked with PCB153 (Chiron AS, Norway).

Techniques: MANN-WHITNEY

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Concentrations of POPs and metals in Yup'ik maternal blood from Alaska

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Blood concentrations of POPs and metals in men and women across the Canadian Arctic

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Concentrations of PCBs and OCs (µg/kg plasma lipid) and metals (µg/L whole blood) among Inuit men and women in Greenland by age groups (years)

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques: Clinical Proteomics

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Trends in blood concentrations of POPs (µg/kg plasma lipid) in pregnant Icelandic women in their third trimester

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques: Clinical Proteomics

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Concentrations of POPs and metals in pregnant women from different regions in Greenland for 2010–2011 and 2013

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Time series of blood POPs concentrations from the Faroe Islands Cohort 1 (1986–1987)

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Time series of blood POPs concentrations from the Faroe Islands Cohort 3 (1998–2000)

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Time series of blood POPs concentrations from the Faroe Islands Cohort 2 (1994–1995)

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Time series of blood POPs concentrations from the Faroe Islands Cohort 5 (2007–2009)

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Concentrations of PCBs and OCs in pregnant women (early pregnancy) from northern Norway (mean parity=0.9 (0–4))

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques: Northern Blot

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Trends of contaminants in breast milk from first-time mothers in southern Finland (non-Arctic), central Finland (non-Arctic) and northern Finland (Arctic)

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques: Northern Blot

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Concentrations of POPs in pregnant women, non-pregnant women and men in the Pechenga District of Murmansk Oblast, 2013–2014

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques:

Journal: International Journal of Circumpolar Health

Article Title: Levels and trends of contaminants in humans of the Arctic

doi: 10.3402/ijch.v75.33804

Figure Lengend Snippet: Circumpolar concentrations of PBDEs, PFOS, PCB153, p,p’ -DDE, Hg and Pb. Unless otherwise indicated, POPs and PBDEs are presented in µg/kg plasma lipid, PFCs in µg/L and metals in µg/L whole blood. Data for Alaska, Faroe Islands, Sweden (except metals) and coastal Chukotka (Russia) are derived from maternal blood. Data for the Inuvialuit Settlement Region (NWT, Canada), Nunavut (Canada) and Nunatsiavut (NL, Canada) are derived from the blood of women of childbearing age. Data for Nunavik (QC, Canada), Greenland, Iceland, Norway, Sweden (metals only) and the Pechenga district (Russia) are derived from the blood of pregnant women. Data for Sweden (PBDE47, PCB153 and p,p’ -DDE) and Finland are derived from breast milk.

Article Snippet: However, Canada also has some of the lowest levels of PCB153, as shown for populations from the Inuvialuit Settlement Region and Nunatsiavut, and for PFOS and PFOA measured in populations from Nunavik.

Techniques: Clinical Proteomics, Derivative Assay

Journal: Environmental Health Perspectives

Article Title: High-Throughput Transcriptomics of Nontumorigenic Breast Cells Exposed to Environmentally Relevant Chemicals

doi: 10.1289/EHP12886

Figure Lengend Snippet: Differential gene expression for 21 chemicals under assessment at 2.5 μ M . Differential gene expression between each chemical concentration and controls was calculated using quasi-likelihood negative binomial generalized log-linear modeling in edgeR. Red dotted lines mark a false discovery rate (FDR) adjusted p -value of 0.05. Cells were exposed to each chemical for 48 h with four biological replicates per concentration. Table S3 contains raw differential gene expression data across all chemicals and concentrations. Note: BPA, bisphenol A; BPF, bisphenol F; BPS, bisphenol S; DCP, dichlorophenol; DCB, dichlorobenzene; DDE, dichlorodiphenyldichloroethylene; FC, fold change; MPB, methylparaben; PCB, polychlorinated biphenyl; PFDA, perfluorodecanoic acid; PFNA, perfluorononanoic acid; PFOA, perfluorooctanoic acid; PFOS, perfluorooctanesulfonic acid; PPB, propylparaben.

Article Snippet: The 21 chemicals [sodium (meta) arsenite, cadmium chloride, copper (II) chloride, lead (II) acetate trihydrate, mercury (II) chloride, phenanthrene, p,p ′-DDE (dichlorodiphenyldichloroethylene), thiram, 1,4-dichlorobenzene, 2,5-dichlorophenol, methylparaben, propylparaben, perfluorodecanoic acid (PFDA), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), polychlorinated biphenyl 153 (PCB153), polychlorinated biphenyl 187 (PCB187), bisphenol A, bisphenol F, bisphenol S] were purchased from Sigma-Aldrich and Cayman Chemicals (Table S1).

Techniques: Gene Expression, Concentration Assay

Journal: Environmental Health Perspectives

Article Title: High-Throughput Transcriptomics of Nontumorigenic Breast Cells Exposed to Environmentally Relevant Chemicals

doi: 10.1289/EHP12886

Figure Lengend Snippet: Differentially expressed genes (DEGs) (FDR < 0.05 relative to control) by concentration for each chemical.

Article Snippet: The 21 chemicals [sodium (meta) arsenite, cadmium chloride, copper (II) chloride, lead (II) acetate trihydrate, mercury (II) chloride, phenanthrene, p,p ′-DDE (dichlorodiphenyldichloroethylene), thiram, 1,4-dichlorobenzene, 2,5-dichlorophenol, methylparaben, propylparaben, perfluorodecanoic acid (PFDA), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), polychlorinated biphenyl 153 (PCB153), polychlorinated biphenyl 187 (PCB187), bisphenol A, bisphenol F, bisphenol S] were purchased from Sigma-Aldrich and Cayman Chemicals (Table S1).

Techniques: Control, Concentration Assay

Journal: Environmental Health Perspectives

Article Title: High-Throughput Transcriptomics of Nontumorigenic Breast Cells Exposed to Environmentally Relevant Chemicals

doi: 10.1289/EHP12886

Figure Lengend Snippet: Benchmark concentration accumulation plots and individual benchmark concentration modeling graphs. Benchmark concentration modeling was performed using BMDExpress software. In the accumulation plot of median best benchmark concentrations (A), each plotted point represents a differentially expressed gene and each differently colored line represents individual chemicals. On the right, example individual best fit benchmark concentration graphs are shown for the gene ARC in arsenic-treated cells (B) and for the gene HSPA6 in thiram-treated cells (C). For B and C, experimental data is shown in red, best fit model in blue, and lower bound of the 95% confidence interval of the benchmark concentration (BMCL), benchmark concentration (BMC), and upper bound of the 95% confidence interval of the benchmark concentration (BMCU) are labeled on each graph. The supplemental bm2 file contains all benchmark concentration modeling data. Note: BPA, bisphenol A; BPF, bisphenol F; BPS, bisphenol S; DCP, dichlorophenol; DCB, dichlorobenzene; DDE, dichlorodiphenyldichloroethylene; FC, fold change; MPB, methylparaben; PCB, polychlorinated biphenyl; PFDA, perfluorodecanoic acid; PFNA, perfluorononanoic acid; PFOA, perfluorooctanoic acid; PFOS, perfluorooctanesulfonic acid; PPB, propylparaben.

Article Snippet: The 21 chemicals [sodium (meta) arsenite, cadmium chloride, copper (II) chloride, lead (II) acetate trihydrate, mercury (II) chloride, phenanthrene, p,p ′-DDE (dichlorodiphenyldichloroethylene), thiram, 1,4-dichlorobenzene, 2,5-dichlorophenol, methylparaben, propylparaben, perfluorodecanoic acid (PFDA), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), polychlorinated biphenyl 153 (PCB153), polychlorinated biphenyl 187 (PCB187), bisphenol A, bisphenol F, bisphenol S] were purchased from Sigma-Aldrich and Cayman Chemicals (Table S1).

Techniques: Concentration Assay, Software, Labeling

Journal: Environmental Health Perspectives

Article Title: High-Throughput Transcriptomics of Nontumorigenic Breast Cells Exposed to Environmentally Relevant Chemicals

doi: 10.1289/EHP12886

Figure Lengend Snippet: Comparison of benchmark concentration modeling results with National Health and Nutrition Examination Survey (NHANES) exposure biomarker levels. Median benchmark concentration (BMC) for each differentially expressed gene (red/textured) and NHANES chemical biomarker concentration data, converted to molarity units, for female participants (blue/solid) plotted for each chemical. Each box represents first quartile, median, and third quartile, the whiskers represent 1.5 × interquartile range , and outliers are also plotted as black dots. Table S6 contains all numerical values for the box and whiskers plots. Note: BPA, bisphenol A; BPF, bisphenol F; BPS, bisphenol S; DCP, dichlorophenol; DCB, dichlorobenzene; DDE, dichlorodiphenyldichloroethylene; FC, fold change; MPB, methylparaben; PCB, polychlorinated biphenyl; PFDA, perfluorodecanoic acid; PFNA, perfluorononanoic acid; PFOA, perfluorooctanoic acid; PFOS, perfluorooctanesulfonic acid; PPB, propylparaben.

Article Snippet: The 21 chemicals [sodium (meta) arsenite, cadmium chloride, copper (II) chloride, lead (II) acetate trihydrate, mercury (II) chloride, phenanthrene, p,p ′-DDE (dichlorodiphenyldichloroethylene), thiram, 1,4-dichlorobenzene, 2,5-dichlorophenol, methylparaben, propylparaben, perfluorodecanoic acid (PFDA), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), polychlorinated biphenyl 153 (PCB153), polychlorinated biphenyl 187 (PCB187), bisphenol A, bisphenol F, bisphenol S] were purchased from Sigma-Aldrich and Cayman Chemicals (Table S1).

Techniques: Comparison, Concentration Assay, Biomarker Discovery

Journal: Environmental Health Perspectives

Article Title: High-Throughput Transcriptomics of Nontumorigenic Breast Cells Exposed to Environmentally Relevant Chemicals

doi: 10.1289/EHP12886

Figure Lengend Snippet: Enrichment analyses. All of Molecular Signature Database’s (MSigDB’s) “hallmark” biological processes showing differences in expression of at least one gene as regulated by each chemical across different median benchmark concentrations (BMCs) representing the BMC modeling averaged result for the gene(s) affected in that hallmark process (A). Twenty chemicals showing significant enrichment or downregulation for 1 or more of 50 biological processes defined by hallmark gene sets (B). Nineteen chemicals showing significant differences in breast cancer–specific or stemness-specific gene pathways as defined by some of MSigDB’s “Chemical and Genetic Perturbations” sets (C). For panels B and C, “conflict” indicates gene expression differences correlated with both up and downregulation of the pathways of interest. BMDExpress-defined category analysis was used for enrichment analysis. Tables S7 contains the enrichment analysis results for . Note: BPA, bisphenol A; BPF, bisphenol F; BPS, bisphenol S; DCP, dichlorophenol; DCB, dichlorobenzene; DDE, dichlorodiphenyldichloroethylene; FC, fold change; MPB, methylparaben; PCB, polychlorinated biphenyl; PFDA, perfluorodecanoic acid; PFNA, perfluorononanoic acid; PFOA, perfluorooctanoic acid; PFOS, perfluorooctanesulfonic acid; PPB, propylparaben.

Article Snippet: The 21 chemicals [sodium (meta) arsenite, cadmium chloride, copper (II) chloride, lead (II) acetate trihydrate, mercury (II) chloride, phenanthrene, p,p ′-DDE (dichlorodiphenyldichloroethylene), thiram, 1,4-dichlorobenzene, 2,5-dichlorophenol, methylparaben, propylparaben, perfluorodecanoic acid (PFDA), perfluorononanoic acid (PFNA), perfluorooctanoic acid (PFOA), perfluorooctanesulfonic acid (PFOS), polychlorinated biphenyl 153 (PCB153), polychlorinated biphenyl 187 (PCB187), bisphenol A, bisphenol F, bisphenol S] were purchased from Sigma-Aldrich and Cayman Chemicals (Table S1).

Techniques: Expressing, Gene Expression