Journal: Molecular cell

Article Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit

doi: 10.1016/j.molcel.2020.11.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fully Sequenced cDNA (Source BioScience) with primers forward 5′-gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-3′ and reverse 5′-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-3′, followed by cloning into the pCAG-IRES-GFP backbone ( Ambrozkiewicz et al., 2018 ) with the restriction enzyme EcoRI (NEB).

Techniques: Recombinant, Luciferase, shRNA, Transfection, Protease Inhibitor, Plasmid Preparation, Mass Spectrometry, RNA Sequencing Assay, Software, Over Expression

Journal: Molecular cell

Article Title: Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit

doi: 10.1016/j.molcel.2020.11.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Fully Sequenced cDNA (Source BioScience) with primers forward 5′-gtctcatcattttggcaaagATGTACCCATACGATGTTCCAGATTACGCTTCGGGCGAAGACGAG-3′ and reverse 5′-cggccgcgatatcctcgaggTCAGTCCCCAGCTCCATTC-3′, followed by cloning into the pCAG-IRES-GFP backbone ( Ambrozkiewicz et al., 2018 ) with the restriction enzyme EcoRI (NEB).

Techniques: Recombinant, Luciferase, shRNA, Transfection, Protease Inhibitor, Plasmid Preparation, Mass Spectrometry, RNA Sequencing Assay, Software, Over Expression

Journal: eNeuro

Article Title: Amyloid-β-Induced Dendritic Spine Elimination Requires Ca 2+ -Permeable AMPA Receptors, AKAP-Calcineurin-NFAT Signaling, and the NFAT Target Gene Mdm2

doi: 10.1523/ENEURO.0175-23.2024

Figure Lengend Snippet: Key reagents and resources

Article Snippet: The pCAG-hAPP695(Swe-Ind) backbone was then doubly digested with these same restriction enzymes (SacI-HF and NotI-HF, NEB).

Techniques: Recombinant, Plasmid Preparation, Software, Imaging