Journal: Journal of Bacteriology

Article Title: H-NS Binding and Repression of the ctx Promoter in Vibrio cholerae ▿

doi: 10.1128/JB.01343-09

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Arabinose was added to the growth medium at a final concentration of 0.02%. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Characteristic(s) Reference or source V. cholerae strains O395 Sm Classical Ogawa, Sm r 62 KSK218 CG842 ctx-lacZ 57 MBN019 KSK218 Δ toxT 47 MBN153 MBN019 Δ hns1 47 E. coli strains ER2566 Expression strain, T7 RNA polymerase New England Biolabs BL21(DE3) Expression strain, hsdS gal (λ imm21 nin5 lacuv5-T7 gene 1) (r B − m B − ) Lab collection MC4100 F − araD139 Δ( argF-lac ) U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR , Sm r 55 DL1976 MC4100 hns651 , Tc r 65 MBN425 MC4100 (λ ctx-lacZYA ) 1 , (−522/+114) This work MBN426 MBN425 hns651 , Tc r This work MBN410 MC4100 (λ ctx-lacZYA ) 2 , (−202/+114) This work MBN411 MBN410 hns651 , Tc r This work MBN392 MC4100 (λ ctx-lacZYA ) 3 , (−118/+114) This work MBN408 MBN392 hns651 , Tc r This work MBN412 MC4100 (λ ctx-lacZYA ) 4 , (−65/+114) This work MBN424 MBN412 hns651 , Tc r This work Plasmids pRS415 lacZYA transcriptional fusion vector 56 pMIN46 pRS415::( ctx-lacZYA ) 1 , Ap r 48 pRHK1 pRS415::( ctx-lacZYA ) 2 , Ap r This work pRHK2 pRS415::( ctx-lacZYA ) 3 , Ap r This work pRHK3 pRS415::( ctx-lacZYA ) 4 , Ap r This work pBAD22 Expression vector, Ap r 17 pBlueScript Cloning vector, Ap r Stratagene pBS- ctx pBlueScript, 316-bp ctx promoter fragment (−202/+114) This work pTXB-1 Expression vector for intein/chitin binding domain fusion, Ap r New England Biolabs pEAS10 pTXB-1, hns Ap r 60 pRH81 pBAD22, 6His toxT 24 pACYC184 Expression plasmid, Cm r 6 pTSS-5 pACYC184 toxT + , Cm r 3 pMMB66EH Expression plasmid, Ap r 12 pRH170 Full-length rpoA in pMMB66EH 24 pRH171 rpoA Δ235 in pMMB66EH 24 Open in a separate window Bacterial strains and plasmids used in this study Strain and plasmid construction.

Techniques: Plasmid Preparation, Expressing, Clone Assay, Binding Assay