Journal: Journal of Cancer

Article Title: Paired box 2 promotes progression of endometrial cancer via regulating cell cycle pathway

doi: 10.7150/jca.22418

Figure Lengend Snippet: PAX2 expression level in stable over-expression or knocking-down cell lines. (A) High malignant endometrial carcinoma cell lines exhibited higher PAX2 mRNA expression than cell lines with low malignant potential: AN3CA>HEC1B>HEC1A>RL952. (B) PAX2-si3 had the best interference effect among 3 PAX2 siRNAs in mRNA level. (C) The protein interference effect of PAX2-si3 was obvious in HEC1A and HEC1B. The expression level of PAX2 in HEC1A was 1. (D) The mRNA and protein level of HEC1B-siPAX2 were lower than HEC1B and HEC1B-siNonTarget. (E) The mRNA and protein level of HEC1A-pCMV-PAX2 were higher than HEC1A and HEC1A-pCMV-neo. ** p <0.0001; * p <0.05.

Article Snippet: Quantitative RT-PCR of PAX2 was performed on EcoTM Real-Time PCR system using TaqMan® Gene Expression Assays (Hs99999903_m1 for β-actin, Hs01057417_m1 for PAX2, Applied biosystems, CA, USA) and KAPA PROBE FAST qPCR mastermix (KAPA Biosystems, Cape Town, South Africa).

Techniques: Expressing, Over Expression

Journal: Journal of Cancer

Article Title: Paired box 2 promotes progression of endometrial cancer via regulating cell cycle pathway

doi: 10.7150/jca.22418

Figure Lengend Snippet: The difference of biological ability of HEC1A, HEC1A-pCMV-neo and HEC1A-pCMV-PAX2. (A) Viability of HEC1A-pCMV-PAX2 was significantly higher than HEC1B and HEC1B-siNonTarget after 48 hours. HEC1A-pCMV-PAX2 migration ability (B) and nvasion ability (C) were significantly higher than that of the negative control groups. (D) HEC1A-pCMV-PAX2 exhibited obvious accumulation in the S phase population in cell cycle. ** p <0.0001; * p <0.05.

Article Snippet: Quantitative RT-PCR of PAX2 was performed on EcoTM Real-Time PCR system using TaqMan® Gene Expression Assays (Hs99999903_m1 for β-actin, Hs01057417_m1 for PAX2, Applied biosystems, CA, USA) and KAPA PROBE FAST qPCR mastermix (KAPA Biosystems, Cape Town, South Africa).

Techniques: Migration, Negative Control

Journal: Journal of Cancer

Article Title: Paired box 2 promotes progression of endometrial cancer via regulating cell cycle pathway

doi: 10.7150/jca.22418

Figure Lengend Snippet: PAX2 promoted endometrial cancer cells progression in xenograft nude mice model. (A) HEC1A, HEC1A-pCMV-neo and HEC1A-pCMV-PAX2 growth situation in nude mice. (B) PAX2 expression level in nude mice subcutaneous tumor. (C) HEC1A-pCMV-PAX2 cells proliferated faster than HEC1A and HEC1A-pCMV-neo cells in vivo. (D) Final weight of HEC1A-pCMV-PAX2 was about 3-times of control groups. (E) HE stain of normal liver tissue and livers with metastatic lesions. (F) HEC1A-pCMV-PAX2 group exhibited visible metastatic lesions of the liver. ** p <0.0001; * p <0.05.

Article Snippet: Quantitative RT-PCR of PAX2 was performed on EcoTM Real-Time PCR system using TaqMan® Gene Expression Assays (Hs99999903_m1 for β-actin, Hs01057417_m1 for PAX2, Applied biosystems, CA, USA) and KAPA PROBE FAST qPCR mastermix (KAPA Biosystems, Cape Town, South Africa).

Techniques: Expressing, In Vivo, Control, H&E Stain

Journal: Journal of Cancer

Article Title: Paired box 2 promotes progression of endometrial cancer via regulating cell cycle pathway

doi: 10.7150/jca.22418

Figure Lengend Snippet: Down-regulation PAX2 inhibited endometrial cancer cells progression in xenograft nude mice model. (A) HEC1B, HEC1B-siNonTarget and HEC1B-siPAX2 growth situation in nude mice. (B) PAX2 expression level in nude mice subcutaneous tumor. (C) HEC1B-siPAX2 proliferated more faster than HEC1B and HEC1B-siNonTarget.(D) Final weight of HEC1B-siPAX2 was lighter than control groups. ** p <0.0001; * p <0.05.

Article Snippet: Quantitative RT-PCR of PAX2 was performed on EcoTM Real-Time PCR system using TaqMan® Gene Expression Assays (Hs99999903_m1 for β-actin, Hs01057417_m1 for PAX2, Applied biosystems, CA, USA) and KAPA PROBE FAST qPCR mastermix (KAPA Biosystems, Cape Town, South Africa).

Techniques: Expressing, Control

Journal: Journal of Cancer

Article Title: Paired box 2 promotes progression of endometrial cancer via regulating cell cycle pathway

doi: 10.7150/jca.22418

Figure Lengend Snippet: Network generation of CDK1, YWHAZ and PRKDC based on significantly differential genes found between cells with PAX2 over-expression and those that lack that over-expression. Red nodes represented up-regulated genes, and blue ones were down-regulated genes.

Article Snippet: Quantitative RT-PCR of PAX2 was performed on EcoTM Real-Time PCR system using TaqMan® Gene Expression Assays (Hs99999903_m1 for β-actin, Hs01057417_m1 for PAX2, Applied biosystems, CA, USA) and KAPA PROBE FAST qPCR mastermix (KAPA Biosystems, Cape Town, South Africa).

Techniques: Over Expression

Journal: Journal of Cancer

Article Title: Paired box 2 promotes progression of endometrial cancer via regulating cell cycle pathway

doi: 10.7150/jca.22418

Figure Lengend Snippet: PAX2 activated the cell cycle pathway via regulating CDK1. (A) The PAX2 binding site in promoter region of CDK1. (B) The PAX2 binding sites in promoter region of YWHAZ. (C) CDK1 and YWHAZ mRNA level were elevated in HEC1A-pCMV-PAX2.(D)The protein expression level of CDK1 and PAX2 in cells. (E) Comparison of cell viability in HEC1A-pCMV-neo, HEC1A-pCMV-PAX2, HEC1A-pCMV-PAX2 non-targeting and HEC1A-pCMV-PAX2 siCDK1 cells.

Article Snippet: Quantitative RT-PCR of PAX2 was performed on EcoTM Real-Time PCR system using TaqMan® Gene Expression Assays (Hs99999903_m1 for β-actin, Hs01057417_m1 for PAX2, Applied biosystems, CA, USA) and KAPA PROBE FAST qPCR mastermix (KAPA Biosystems, Cape Town, South Africa).

Techniques: Binding Assay, Expressing, Comparison

Journal: PLoS ONE

Article Title: Retinoic Acid Receptor-Dependent, Cell-Autonomous, Endogenous Retinoic Acid Signaling and Its Target Genes in Mouse Collecting Duct Cells

doi: 10.1371/journal.pone.0045725

Figure Lengend Snippet: Assay ID and amplicon length of Taqman® Gene Expression Assays used.

Article Snippet: 16. , Pax2 , Mm01217939_ m1 , 55.

Techniques: Amplification, Gene Expression

Journal: Science Advances

Article Title: Switch of serotonergic descending inhibition into facilitation by a spinal chloride imbalance in neuropathic pain

doi: 10.1126/sciadv.abo0689

Figure Lengend Snippet: ( A ) GFP (green) and PAX2 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification showing contacts between Pax2 and 5-HT fibers. Scale bar, 50 μm. ( B ) GFP (green) and Tlx3 (red) immunolabeling in the DH. Scale bar, 100 μm. Inset: high magnification, low or no contact. Scale bar, 25 μm. ( C ) After 3D reconstruction (see Materials and Methods) quantification of contacts between cell bodies (Pax2 and Tlx3) and 5-HT fibers. ( C ) Contacted cells are significantly higher for Pax2(+) than for Tlx3(+) in both males and females [males (blue): 86.2 ± 4.6% for Pax2 and 15.5 ± 3.8% for Tlx3, P < 0.001, Mann-Whitney; females (red): 76.3 ± 2.9% for Pax2 and 8.3 ± 2.2% for Tlx3, P < 0.001, Mann-Whitney]. ( D ) Immunostaining against TPH2 in slices of lumbar spinal cord of GAD67::GFP mice. Scale bar, 25 μm. GABA neurons [GFP(+) in green, stars] or fibers in DH are contacted by TPH2-positive putative synaptic button (in red, white arrows). ( E ) Expression of Myc-tag in synaptic 5-HT terminals in DH following injections of an AAV-synaptophysin-Myc. Immunoreactivity is present in the deeper lamina of the DHSC. Scale bar, 100 μm. Higher magnification shows 5-HT axonic button (white arrow). Scale bar, 20 μm. ( F ) Myc(+) synaptic buttons are in direct contact with GAD67-positive cells and fibers in epet::cre/ GAD67::GFP mice. Scale bar, 20 μm. Inset (top): Myc(+) button on GAD(+) cell bodies. Inset (bottom): Myc(+) button on GAD(+) on GAD(+) fibers.

Article Snippet: For molecular identifications of the spinal network, spinal cord sections were incubated free-floating in 0.1 M PBS containing Triton X-100 (0.3%), bovine serum albumin (1%; Sigma-Aldrich), guinea pig anti-TLX3 antibody (1:1000; a gift from T. Müller), goat anti-Pax2 antibody (1:300; Bio-techne), rabbit anti-Myc antibody (1:500; Euromedex), rabbit anti-TPH2 antibody (1:1000; Bio-techne), and rabbit anti-GAD65/67 (G5163, Sigma-Aldrich) in combination with chicken anti-GFP antibody (1:1000; Averlabs) overnight at 4°C.

Techniques: Immunolabeling, MANN-WHITNEY, Immunostaining, Expressing