Journal: STAR protocols

Article Title: Protocol for differential multi-omic analyses of distinct cell types in the mouse cerebral cortex.

doi: 10.1016/j.xpro.2023.102793

Figure Lengend Snippet: Figure 1. In utero electroporation of an anaesthetized mouse (A) Workspace setup, cold source lamps, and surgery tools for in utero electroporation. Insert: Pulled borosilicate micropipettes stored on play dough in a 15-cm cell culture plate. (B) Right before surgery, the mouse is anesthetized in an isoflurane chamber and the eyes are covered with Vidisic gel to prevent drying out of eyes. (C) Fixation of the mouse with lab tape on its back on a heating pad with an isoflurane vaporizer nozzle fitting on the nose of the mouse.

Article Snippet: This method is considered the most sensitive indicator of complete analgesia.30 Note: The isoflurane-O2 mixed air coming out of the isoflurane chamber and any excess isoflurane-O2 mixed air passing through the ventilator tube/nozzle is passed through the activated charcoal isoflurane adsorber (Stoelting) to prevent releasing any isoflurane into the surroundings.

Techniques: In Utero, Electroporation, Cell Culture