Journal:

Article Title: Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension

doi: 10.1073/pnas.0405908101

Figure Lengend Snippet: The mRNA expression of TRPC channels in NPH lung tissue and PASMC. (A) Representative and summarized (n = 4) mRNA products for TRPC genes and GAPDH in NPH human lung. (B) Representative and summarized (n = 3) data showing TRPC gene mRNA products in human brain tissue (B), PASMC (S), and PAEC (E). M, 100-bp DNA ladder.

Article Snippet: Human PASMC from normal subjects (NPH) were purchased from Cambrex, cultured at 37°C in SMGM, and used at the fourth to sixth passage.

Techniques: Expressing

Journal:

Article Title: Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension

doi: 10.1073/pnas.0405908101

Figure Lengend Snippet: TRPC6 mRNA and protein expression is increased in pulmonary tissues from IPAH patients. (A) Representative and summarized (NPH, n = 3; SPH, n = 8; IPAH, n = 3; normalized to α-actin) TRPC6 protein expression in SPH, NPH, and IPAH lung tissues and their α-actin controls. (B) Representative and summarized (n = 3 for each) data showing TRPC6 mRNA products in isolated NPH-, SPH-, or IPAH-PASMC. M, 100-bp DNA ladder. (C) Representative and summarized (n = 5 for each) TRPC6 protein expression in isolated PASMC from NPH, SPH, and IPAH patients. (D) Representative (Top, ×40; Middle, ×100) and summarized immunofluorescence staining of FITC-conjugated TRPC6 (Bottom) in isolated NPH-(n = 11), SPH (n = 21), and IPAH-(n = 36) PASMC. (Scale bars, 20 μm.) ***, P < 0.001 vs. NPH and/or SPH.

Article Snippet: Human PASMC from normal subjects (NPH) were purchased from Cambrex, cultured at 37°C in SMGM, and used at the fourth to sixth passage.

Techniques: Expressing, Isolation, Immunofluorescence, Staining

Journal:

Article Title: Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension

doi: 10.1073/pnas.0405908101

Figure Lengend Snippet: Up-regulated TRPC3 expression in IPAH-PASMC. (A) TRPC3 mRNA expression in PASMC from two NPH patients and two IPAH patients. TRPC3 and -4 mRNA expression in PASMC from two IPAH patients and two SPH patients. M, 100-bp DNA ladder. (B) TRPC3 protein expression in the same IPAH and SPH samples as shown in A. (C) Summarized bar graphs (n = 3 for each) depicting normalized TRPC3 mRNA and protein expression. ***, P < 0.001 vs. IPAH.

Article Snippet: Human PASMC from normal subjects (NPH) were purchased from Cambrex, cultured at 37°C in SMGM, and used at the fourth to sixth passage.

Techniques: Expressing

Journal: BMC Pulmonary Medicine

Article Title: Bmi-1 alleviates adventitial fibroblast senescence by eliminating ROS in pulmonary hypertension

doi: 10.1186/s12890-021-01439-0

Figure Lengend Snippet: Fibroblasts Bmi-1 alters PASMC proliferation by paracrine mode of action. a – b . EDU staining for PASMCs treated with the CM of HLFs. HLFs were infected with adv-Bmi-1 or transfected with si-Bmi-1 following by hypoxia exposure for 96 h, and then the supernatant was collected and added the equal volume of serum-free culture medium to culture PASMCs for 48 h. All data are shown as the mean ± SEM of at least three independent experiments. Statistical significance was assessed using the unpaired two-tailed Student’s t test: * P < 0.05

Article Snippet: The human pulmonary artery smooth muscle cell line (PASMC) and human lung fibroblast cell line (HLF) were purchased from Lonza (Swiss).

Techniques: Staining, Infection, Transfection, Two Tailed Test

Journal: Radiation research

Article Title: Molecular Changes in miRNA in Irradiated Rat Kidneys: Role of miR-34a and its Vascular Targets in the Notch Pathway

doi: 10.1667/RADE-20-00078.1

Figure Lengend Snippet: Top findings of miR-34a-5p (and other miRNAs with seed GGCAGUG) (mammalian) from Ingenuity Knowledge Base

Article Snippet: Regulated by: , TP53, sirolimus, MECOM, PASMC cells, VPR, RPL22, DICER1, TMEM8B, 5-fluorouracil, AGO2, CCNE1, Wi 38 cells, Mrc 5 cells, CEBPA, valproic acid.

Techniques: Expressing, Migration