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Image Search Results
Journal: Molecular and Cellular Biology
Article Title: Crumbs3 Is Essential for Proper Epithelial Development and Viability
doi: 10.1128/MCB.00999-13
Figure Lengend Snippet: Evaluation of epithelial polarity in Crumbs3 knockout lungs. (A) Frozen sections from E18.5 lungs were stained for Pals1, ZO1, and occludin (×600; zoom, 1.5). (B) Western blot of Crb complex proteins Pals1 and Patj in E18.5 lungs. β-Actin was used as a loading control. Samples were run on nonadjacent lanes of the same gel. (C) Frozen sections from E18.5 +/+ and −/− mouse lungs were stained for AQP5 (alveolar type I cells) (×600; zoom, 2), acetylated tubulin (cilia) (zoom, 1.5), Muc1 (apical surface) (zoom, 1.5), ezrin and phospho-ERM (cytoskeleton) (zoom, 3), E-cadherin and β-catenin (adherens junction/basolateral surface) (zoom, 1.5). (D) Frozen sections from E18.5 +/+ and −/− mouse lungs were stained for Par3 (×600) (zoom, 3) and aPKC (apical polarity complex) (zoom, 1.5).
Article Snippet: The primary antibodies used were acetylated tubulin (Sigma, St. Louis, MO; catalog no. T7451), aPKC (R&D, Minneapolis, MN; catalog no. AF4465), Aquaporin 1 (Aqp1) (Santa Cruz, Santa Cruz, CA; catalog no. SC-25287), Aqp2 (Santa Cruz; catalog no. SC-9882), Aqp5 (Abcam, Cambridge, MA; catalog no. AB78486), α-tubulin (Sigma; catalog no. T6199), β-actin (Sigma; catalog no. A2228), β-catenin (BD Bioscience, San Jose, CA; catalog no. 610154), CC10 (Santa Cruz; catalog no. SC-9772), chemokine C-C motif ligand 2 (CCL2) (R&D; catalog no. AF479-NA), claudin-4 (Santa Cruz; catalog no. SC-17664), cleaved caspase-3 (Cell Signaling, Danvers, MA; catalog no. 9661), Crumbs3a (Custom [ 24 ]), E-cadherin (R&D; catalog no. AF748), ERM (Cell Signaling; catalog no. 3142), ezrin (Sigma; catalog no. E8897), GFP-agarose (MBL, Woburn, MA; catalog no. D153-8), hemagglutinin (HA) (Santa Cruz; catalog no. SC-805), Hes1 (MBL; catalog no. D134-3), Ki67 (Abcam; catalog no. AB16667), mucin 1 (Muc1) (Novus Biologicals, Littleton, CO; catalog no. NB120-15481), Nkx2.1 (Epitomics, Burlingame, CA; catalog no. 2044-1), occludin (Invitrogen; catalog no. 71-1500), Pals1 for immunoblotting (IB) (Santa Cruz; catalog no. SC-365411), Pals1 for immunohistochemistry (IHC) (Custom [ 25 ]),
Techniques: Knock-Out, Staining, Western Blot, Control
Journal: Molecular and Cellular Biology
Article Title: Crumbs3 Is Essential for Proper Epithelial Development and Viability
doi: 10.1128/MCB.00999-13
Figure Lengend Snippet: Evaluation of epithelial polarity and signaling pathways in Crumbs3 knockout kidneys. (A) Frozen sections from E17.5 kidneys were stained for Pals1 (green), ZO1 (red), and E-cadherin (blue) (×400 magnification). (B) Western blot of Crb complex protein Pals1 in E18.5 kidneys. β-Actin was used as a loading control. Samples were run on nonadjacent lanes of the same gel. (C) Frozen sections of kidneys from E17.5 to E18.5 mice were stained for ezrin (cytoskeleton), occludin and ZO1 (tight junction), E-cadherin (adherens junction/basolateral surface), claudin-4 (tight junction), Par3, and aPKC (apical polarity complex) (×600; zoom, 1.5). (D) Western blot of p-ERM and ERM protein levels among +/+ and −/− kidneys at E18.5. β-Actin and α-tubulin are shown as loading controls. Samples were run on nonadjacent lanes of the same gel. (E and F) Western blots of p-Yap1 (Hippo pathway activation) and Hes1 (Notch pathway activation) among +/+ and −/− kidneys at E18.5. α-Tubulin is shown as the loading control. Samples were run on nonadjacent lanes of the same gel.
Article Snippet: The primary antibodies used were acetylated tubulin (Sigma, St. Louis, MO; catalog no. T7451), aPKC (R&D, Minneapolis, MN; catalog no. AF4465), Aquaporin 1 (Aqp1) (Santa Cruz, Santa Cruz, CA; catalog no. SC-25287), Aqp2 (Santa Cruz; catalog no. SC-9882), Aqp5 (Abcam, Cambridge, MA; catalog no. AB78486), α-tubulin (Sigma; catalog no. T6199), β-actin (Sigma; catalog no. A2228), β-catenin (BD Bioscience, San Jose, CA; catalog no. 610154), CC10 (Santa Cruz; catalog no. SC-9772), chemokine C-C motif ligand 2 (CCL2) (R&D; catalog no. AF479-NA), claudin-4 (Santa Cruz; catalog no. SC-17664), cleaved caspase-3 (Cell Signaling, Danvers, MA; catalog no. 9661), Crumbs3a (Custom [ 24 ]), E-cadherin (R&D; catalog no. AF748), ERM (Cell Signaling; catalog no. 3142), ezrin (Sigma; catalog no. E8897), GFP-agarose (MBL, Woburn, MA; catalog no. D153-8), hemagglutinin (HA) (Santa Cruz; catalog no. SC-805), Hes1 (MBL; catalog no. D134-3), Ki67 (Abcam; catalog no. AB16667), mucin 1 (Muc1) (Novus Biologicals, Littleton, CO; catalog no. NB120-15481), Nkx2.1 (Epitomics, Burlingame, CA; catalog no. 2044-1), occludin (Invitrogen; catalog no. 71-1500), Pals1 for immunoblotting (IB) (Santa Cruz; catalog no. SC-365411), Pals1 for immunohistochemistry (IHC) (Custom [ 25 ]),
Techniques: Protein-Protein interactions, Knock-Out, Staining, Western Blot, Control, Activation Assay