palmtracer Search Results


palmtracer plugin  (MetaMorph Inc)
90
MetaMorph Inc palmtracer plugin
PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using <t>PALMTracer</t> from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.
Palmtracer Plugin, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/palmtracer plugin/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
palmtracer plugin - by Bioz Stars, 2026-05
90/100 stars
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custom-written software operating as a plug-in within metamorph software palmtracer  (MetaMorph Inc)
90
MetaMorph Inc custom-written software operating as a plug-in within metamorph software palmtracer
PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using <t>PALMTracer</t> from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.
Custom Written Software Operating As A Plug In Within Metamorph Software Palmtracer, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom-written software operating as a plug-in within metamorph software palmtracer/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
custom-written software operating as a plug-in within metamorph software palmtracer - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
software operating as a plug-in within metamorph software palmtracer  (MetaMorph Inc)
90
MetaMorph Inc software operating as a plug-in within metamorph software palmtracer
PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using <t>PALMTracer</t> from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.
Software Operating As A Plug In Within Metamorph Software Palmtracer, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software operating as a plug-in within metamorph software palmtracer/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
software operating as a plug-in within metamorph software palmtracer - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using PALMTracer from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.

Journal: Microbiology Spectrum

Article Title: Imaging Minimal Bacteria at the Nanoscale: a Reliable and Versatile Process to Perform Single-Molecule Localization Microscopy in Mycoplasmas

doi: 10.1128/spectrum.00645-22

Figure Lengend Snippet: PALM imaging of an F-type ATPase in Mycoplasma mycoides subsp. capri . M. mycoides subsp. capri mEos3.2-0575 cells, expressing a mEos-fused variant of the β-subunit of the ATPase F 1-like domain, were imaged by PALM. In this M. mycoides subsp. capri mutant, the fluorescent fusion protein is expressed from the native genomic locus and replaces the wild-type variant. The data presented here correspond to a single representative field of view (512 by 512 pixels; pixel size = 160 nm). (A) Sample images of M. mycoides subsp. capri mEos3.2-0575 cells. For each field of view, the images correspond to epifluorescence (diffraction limited) (top), superresolved reconstruction (40-nm pixel) (middle), and Tesseler segmentation (bottom). Scale bar = 1 μm. (B) Tesseler clustering of the fluorescence signal. The number of clusters per Tesseler-segmented object was computed. The bar graphs display the distribution of the numbers of clusters per object. (C) Object and cluster sizes. The dot plot presents the area (in nm 2 ) of each object and cluster segmented by Tesseler, to which a boxplot showing the median, interquartile range, minimum, and maximum values is overlaid. The median value of each data set is indicated. (D) Evaluation of the PALM imaging pointing accuracy. Inset, example of the tracks computed using PALMTracer from which the MSD 0 and pointing accuracy values are derived. The bar graphs display the distribution of the pointing accuracies derived from each track. The median value of the data set is indicated.

Article Snippet: The PALMTracer plugin for MetaMorph ( ) was used to process image stacks with a specific intensity threshold for each data set, to enable the generation of the localization tables.

Techniques: Imaging, Expressing, Variant Assay, Mutagenesis, Fluorescence, Derivative Assay