Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: On-Chip Acousto Thermal Shift Assay for Rapid and Sensitive Assessment of Protein Thermodynamic Stability.

doi: 10.1002/smll.202003506

Figure Lengend Snippet: Figure 2. The ATSA enables rapid and sensitive assessment of protein–ligand binding and protein stability. A–C), Representative melting curves and melting time shifts Δtm detected by our ATSA technique in (A) (n = 4), and melting temperature shifts ΔTm detected by two conventional TSA methods, i.e., SYPRO differential scanning fluorimetry (DSF) assay in (B) (n = 4) and bicinchoninic acid (BCA) in (C) (n = 3), for two purified proteins, Hb and CS, in the absence or presence of their corresponding binding ligands, i.e., palmatine chloride (Pal) and oxaloacetic acid (OAA). The arrows in (A) indicate the melting time at which the 50% change of gray intensity (Im = (Imax + Imin)/2) occurs for each curve. The Hb concentration of 31 × 10−6 m and the

Article Snippet: Materials and Reagents: Two proteins, i.e., human hemoglobin (Hb; Millipore Sigma, St. Louis, MO) and citrate synthase (CS) from porcine heart (Millipore Sigma), and two corresponding compounds, i.e., palmatine chloride (Pal; Santa Cruz Biotechnology, Santa Cruz, CA) and oxaloacetic acid (OAA; Millipore Sigma) were primarily used in this study.

Techniques: Ligand Binding Assay, Purification, Binding Assay, Concentration Assay

Journal: Pharmaceuticals

Article Title: Sinomenium acutum Modulates Platelet Aggregation and Thrombus Formation by Regulating the Glycoprotein VI-Mediated Signalosome in Mice

doi: 10.3390/ph17010006

Figure Lengend Snippet: Phytochemical components of Sinomenium acutum via UHPLC-MS/MS.

Article Snippet: The latter included higenamine, gelsemine, magnoflorine, scopoletin, columbamine, jatrorrhizine, palmatine, syringin, and eleutheroside E, which were purchased from TargetMol (Wellesley Hills, MA, USA).

Techniques:

Journal: Pharmaceuticals

Article Title: Sinomenium acutum Modulates Platelet Aggregation and Thrombus Formation by Regulating the Glycoprotein VI-Mediated Signalosome in Mice

doi: 10.3390/ph17010006

Figure Lengend Snippet: Ultra-high-performance liquid chromatography–tandem mass spectrometry examination of Sinomenium acutum . ( A ) Base peak chromatogram of Sinomenium acutum in positive and negative ion mode. ( B ) Elicit ion chromatogram of Sinomenium acutum . 1, higenamine; 2, acutumidine; 3, acutumine; 4, sinomenine; 5, gelsemine; 6, magnoflorine; 7, scopoletin; 8, columbamine or jatrorrhizine; 9, palmatine; 10, syringin; 11, eleutheroside E.

Article Snippet: The latter included higenamine, gelsemine, magnoflorine, scopoletin, columbamine, jatrorrhizine, palmatine, syringin, and eleutheroside E, which were purchased from TargetMol (Wellesley Hills, MA, USA).

Techniques: High Performance Liquid Chromatography, Mass Spectrometry

Journal: Journal of ethnopharmacology

Article Title: Probing PXR activation and modulation of CYP3A4 by Tinospora crispa and Tinospora sinensis

doi: 10.1016/j.jep.2022.115159

Figure Lengend Snippet: PXR activation by phytochemicals of T. crispa and T. sinensis .

Article Snippet: Jatrorrhizine and palmatine were purchased from Wuhan ChemFaces Biochemical Co., Ltd (China).

Techniques: Activation Assay, Activity Assay

Journal: Journal of ethnopharmacology

Article Title: Probing PXR activation and modulation of CYP3A4 by Tinospora crispa and Tinospora sinensis

doi: 10.1016/j.jep.2022.115159

Figure Lengend Snippet: In vitro CYP3A4 inhibition by phytochemicals of T. crispa and T. sinensis .

Article Snippet: Jatrorrhizine and palmatine were purchased from Wuhan ChemFaces Biochemical Co., Ltd (China).

Techniques: In Vitro, Inhibition, Positive Control

Journal: Journal of ethnopharmacology

Article Title: Probing PXR activation and modulation of CYP3A4 by Tinospora crispa and Tinospora sinensis

doi: 10.1016/j.jep.2022.115159

Figure Lengend Snippet: MMGBSA energy calculated for the test compounds and their hypothetical aglycone.

Article Snippet: Jatrorrhizine and palmatine were purchased from Wuhan ChemFaces Biochemical Co., Ltd (China).

Techniques:

Journal: Chinese Herbal Medicines

Article Title: Pharmacokinetic studies of multi-bioactive components in rat plasma after oral administration of Xintiantai Ⅰ extract and effects of guide drug borneol on pharmacokinetics

doi: 10.1016/j.chmed.2019.06.003

Figure Lengend Snippet: Multiple reactions monitoring mode chromatograms of G-Rg1, Rb1, Rd, berberine, epiberberine, jatrorrhizine, palmatine, columbamine, coptisine, evodiamine, dehydroevodiamine, rutaecarpine, limonin, hyperin, curcumin, demethoxycurcumin, bisdemethoxycurcumin, and naringin in blank plasma (A), blank methanol spiked with standards solution (B) and rat plasma sample after oral administration of Xintiantai Ⅰ (C).

Article Snippet: Ginsenoside Rg1, Rb1, and Rd, berberine, epiberberine, jatrorrhizine, palmatine, columbamine, coptisine, evodiamine, dehydroevodiamine, rutaecarpine, limonin, hyperin, curcumin, demethoxycurcumin, bisdemethoxycurcumin and naringin (used as internal standard, IS) were purchased from Baoji Chenguang Biological Technology Co., Ltd.

Techniques: Clinical Proteomics

Journal: Chinese Herbal Medicines

Article Title: Pharmacokinetic studies of multi-bioactive components in rat plasma after oral administration of Xintiantai Ⅰ extract and effects of guide drug borneol on pharmacokinetics

doi: 10.1016/j.chmed.2019.06.003

Figure Lengend Snippet: Summary of matrix effect, recovery, precision, and accuracy of all analytes for UHPLC–MS/MS method in rat plasma (mean ± SD, n = 6).

Article Snippet: Ginsenoside Rg1, Rb1, and Rd, berberine, epiberberine, jatrorrhizine, palmatine, columbamine, coptisine, evodiamine, dehydroevodiamine, rutaecarpine, limonin, hyperin, curcumin, demethoxycurcumin, bisdemethoxycurcumin and naringin (used as internal standard, IS) were purchased from Baoji Chenguang Biological Technology Co., Ltd.

Techniques: Clinical Proteomics

Journal: Chinese Herbal Medicines

Article Title: Pharmacokinetic studies of multi-bioactive components in rat plasma after oral administration of Xintiantai Ⅰ extract and effects of guide drug borneol on pharmacokinetics

doi: 10.1016/j.chmed.2019.06.003

Figure Lengend Snippet: Pharmacokinetic parameters of 17 bioactive components after oral administration of Xintiantai I and Xintiantai I without borneol extracts (mean ± SD, n = 6).

Article Snippet: Ginsenoside Rg1, Rb1, and Rd, berberine, epiberberine, jatrorrhizine, palmatine, columbamine, coptisine, evodiamine, dehydroevodiamine, rutaecarpine, limonin, hyperin, curcumin, demethoxycurcumin, bisdemethoxycurcumin and naringin (used as internal standard, IS) were purchased from Baoji Chenguang Biological Technology Co., Ltd.

Techniques: