Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Activating mutations in ALK kinase domain confer resistance to structurally unrelated ALK inhibitors in NPM-ALK-positive anaplastic large-cell lymphoma

doi: 10.1007/s00432-014-1589-3

Figure Lengend Snippet: Identification of the resistance mechanism in Karpas299CR and Karpas299CHR cells. a Analysis of phosphorylation status of key signaling molecules assessed by the PathScan ® RTK Signaling Antibody Array. b Immunoblot analysis of pALK and downstream signaling proteins following 24 h of incubation in the inhibitor-free medium. c Sequencing of ALK KD coding fragment in Karpas299CR and Karpas299CHR cells

Article Snippet: Primary antibodies against pALK (Y1604), total ALK, pSTAT3 (Y705), total STAT3, pERK1/2 (T202/Y204), total ERK1/2 and pS6 (S240/244) were purchased from Cell Signaling Technology.

Techniques: Phospho-proteomics, Ab Array, Western Blot, Incubation, Sequencing

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Activating mutations in ALK kinase domain confer resistance to structurally unrelated ALK inhibitors in NPM-ALK-positive anaplastic large-cell lymphoma

doi: 10.1007/s00432-014-1589-3

Figure Lengend Snippet: Sensitivity of Karpas299wt and resistant cells to ALK inhibitors. a ATPlite cell viability assay following 72 h of incubation with ALK inhibitors. Data expressed as percentage viability relative to untreated controls. Corresponding IC 50 values are summarized in Table . b BrdU cell proliferation assay and flow cytometry analysis following 48-h incubation with inhibitors. Data expressed as percentage of cells in S phase relative to untreated control. c Immunoblot analysis of pALK and downstream signaling following incubation with inhibitors for 2 h

Article Snippet: Primary antibodies against pALK (Y1604), total ALK, pSTAT3 (Y705), total STAT3, pERK1/2 (T202/Y204), total ERK1/2 and pS6 (S240/244) were purchased from Cell Signaling Technology.

Techniques: Viability Assay, Incubation, BrdU Cell Proliferation Assay, Flow Cytometry, Control, Western Blot