Journal: Shock (Augusta, Ga.)

Article Title: Restorative mechanisms regulating protein balance in skeletal muscle during recovery from sepsis

doi: 10.1097/SHK.0000000000000762

Figure Lengend Snippet: A-F: Representative Western blots and quantitation are presented for phosphorylated and/or total Akt, PRAS40, TSC2, AMPK, and REDD1. The final panel is a representative tubulin blot demonstrating equal protein loading. Loading controls (tubulin or actin) were run for all proteins examined, but only representative data are shown. Bar graphs: were quantified for phosphorylation of each protein normalized to the total amount of the respective protein, except for REDD1 where total protein was normalized to tubulin. Control values were set to 100 arbitrary units (AU). Values are mean ± SEM. *P < 0.05 sepsis-recovery compared to pair-fed controls values; n = 17 and 10, respectively.

Article Snippet: Antibodies included (Cell Signaling, Beverly, MA, unless otherwise noted): S6K1, S6K1 (Thr389), rpS6, rpS6 (Ser240/244 and Ser230/235), 4E-BP1 (Bethyl Laboratories, Montgomery, TX), 4E-BP1 (Ser65), eukaryotic elongation factor (eEF)2, eEF2 (Thr56), eEF2 kinase, eEF2K (Ser366; Dr. Chris Proud), ERK (extracellular signal-regulated kinses), ERK1/2 (Thr202/Tyr204), RSK (90 kDa ribosomal S6 kinase), RSK1/2 (Ser380), REDD1 (regulated in development and DNA damage responses; ProteinTech, Chicago, IL), Akt, Akt (Thr308 and Ser473), PRAS40 (proline-rich Akt substrate of 40 kDa), PRAS40 (Thr246), eIF4B, eIF4B (Ser422), AMP-activated protein kinase-α (AMPK), AMPKα (Thr172), tuberous sclerosis complex 2 (TSC2), TSC2 (Thr1462), Unc-51 like autophagy activating kinase 1 (ULK1), ULK1 (Ser757), p62 (aka SQSTM1), light-chain 3B (LC3B)-I and –II, and MyoD and myogenin.

Techniques: Western Blot, Quantitation Assay

Journal: Journal of Cancer

Article Title: Gambogic acid affects ESCC progression through regulation of PI3K/AKT/mTOR signal pathway

doi: 10.7150/jca.41115

Figure Lengend Snippet: Expression of apoptosis-related proteins and PI3K/AKT/mTOR pathway components in ESCC cells incubated with GA. (A) The expression levels of cleaved-PARP1, Bcl-2, Bax, cleaved-caspase 3 and cleaved-caspase9 were detected by using western blot analysis. (B) Western blotting was performed to analyze the levels of PI3K, p-AKT, p-mTOR, AKT, mTOR and PTEN.

Article Snippet: The following primary antibodies were applied in the experiments: cleaved-caspase3, cleaved-caspase9, cleaved-PARP1, phosphorylated AKT, Bcl-2, MMP2, MMP9 and β-actin (Proteintech Group, Wuhan, China); PARP1, BAX, PI3K, total AKT, phosphorylated AKT (ser473), p-mTOR (ser2448), cyclinB1 and cyclinD1 (Cell Signaling Technology, MA, USA). β-actin was chosen as a loading control in all experiments.

Techniques: Expressing, Incubation, Western Blot

Journal: Frontiers in Systems Neuroscience

Article Title: Imatinib Mesylate Reduces Neurotrophic Factors and pERK and pAKT Expression in Urinary Bladder of Female Mice With Cyclophosphamide-Induced Cystitis

doi: 10.3389/fnsys.2022.884260

Figure Lengend Snippet: Antibodies used for immunohistochemistry.

Article Snippet: Sheep anti-pAKT , 1:3000 , Osenses , CY3 donkey anti-sheep , 1:500 , Jackson Immunoresearch.

Techniques: Immunohistochemistry