pak4 p 21 Search Results


pak4 p 21  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology pak4 p 21
Effect of two <t>PAK4</t> inhibitors, PF-3758309 or LCH-7749944, on the ability of CCK-8 (0.3 and 100 nM) or TPA (1 µM) to alter activation of cofilin. Isolated pancreatic acini were incubated in the absence or presence of PF-3758309 (0.1 nM) or LCH-7749944 (30 µM) for 3 h and then incubated with no additions (control), CCK-8 (0.3 and 100 nM) for 3 min or TPA (1 µM) for 5 min, and then lysed. Western blots were analyzed using anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors; N.S. , No significant.
Pak4 P 21, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pak4+p+21/p21/pmc10149936-19-3-14
Average 96 stars, based on 1 article reviews
pak4 p 21 - by Bioz Stars, 2026-09
96/100 stars
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p21 protein activated kinase 4 pak4 antibody  (Abbexa)
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Polyclonal Antibody to p21 Protein Activated Kinase 4 PAK4
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Effect of two PAK4 inhibitors, PF-3758309 or LCH-7749944, on the ability of CCK-8 (0.3 and 100 nM) or TPA (1 µM) to alter activation of cofilin. Isolated pancreatic acini were incubated in the absence or presence of PF-3758309 (0.1 nM) or LCH-7749944 (30 µM) for 3 h and then incubated with no additions (control), CCK-8 (0.3 and 100 nM) for 3 min or TPA (1 µM) for 5 min, and then lysed. Western blots were analyzed using anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors; N.S. , No significant.

Journal: Frontiers in Physiology

Article Title: Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth

doi: 10.3389/fphys.2023.1147572

Figure Lengend Snippet: Effect of two PAK4 inhibitors, PF-3758309 or LCH-7749944, on the ability of CCK-8 (0.3 and 100 nM) or TPA (1 µM) to alter activation of cofilin. Isolated pancreatic acini were incubated in the absence or presence of PF-3758309 (0.1 nM) or LCH-7749944 (30 µM) for 3 h and then incubated with no additions (control), CCK-8 (0.3 and 100 nM) for 3 min or TPA (1 µM) for 5 min, and then lysed. Western blots were analyzed using anti-pS3 cofilin. Bands were visualized using chemiluminescence and quantified by densitometry. Top : Results of a representative blot of four independent experiments are shown. Bottom : Means ± S.E. of at least 4 independent experiments. Results are expressed as % of basal stimulation of the control group. *, p < 0.05 compared to the control group; ∞, p < 0.05 compared to stimulants without inhibitors; N.S. , No significant.

Article Snippet: Sennoside A (SE), PAK4 (P-21), anti-goat-HRP-conjugate antibodies, calyculin A and cofilin antibody were from Santa Cruz Biotechnology, Inc. (Dallas, TX).

Techniques: CCK-8 Assay, Activation Assay, Isolation, Incubation, Control, Western Blot

Schematic diagram of signaling cascade for activation of cofilin in pancreatic acinar cells. In rat pancreatic acinar cells, maximal activation of cofilin by cholecystokinin (CCK)-8 requires activation of PKC, which mediates both Src family of kinase (SFK) and protein kinase D (PKD) activation resulting in PAK4 activation; requires activation of ROCK, which is mediated by Rho; requires activation of JNK, mediated by activation of this MAPK pathway; and requires activation of serine protein phosphatases (PP2A), which are a substrate of PKC. Cofilin activation is important for CCK-stimulated enzyme secretion as well as ERK1/2 activation which has been shown to mediate growth. Squares represent signaling pathways shown to be involved in cofilin activation in this study.

Journal: Frontiers in Physiology

Article Title: Cofilin activation in pancreatic acinar cells plays a pivotal convergent role for mediating CCK-stimulated enzyme secretion and growth

doi: 10.3389/fphys.2023.1147572

Figure Lengend Snippet: Schematic diagram of signaling cascade for activation of cofilin in pancreatic acinar cells. In rat pancreatic acinar cells, maximal activation of cofilin by cholecystokinin (CCK)-8 requires activation of PKC, which mediates both Src family of kinase (SFK) and protein kinase D (PKD) activation resulting in PAK4 activation; requires activation of ROCK, which is mediated by Rho; requires activation of JNK, mediated by activation of this MAPK pathway; and requires activation of serine protein phosphatases (PP2A), which are a substrate of PKC. Cofilin activation is important for CCK-stimulated enzyme secretion as well as ERK1/2 activation which has been shown to mediate growth. Squares represent signaling pathways shown to be involved in cofilin activation in this study.

Article Snippet: Sennoside A (SE), PAK4 (P-21), anti-goat-HRP-conjugate antibodies, calyculin A and cofilin antibody were from Santa Cruz Biotechnology, Inc. (Dallas, TX).

Techniques: Activation Assay, CCK-8 Assay, Protein-Protein interactions