packaging line Search Results


retroviral packaging cell line  (ATCC)
92
ATCC retroviral packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Retroviral Packaging Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phoenixeco ecotropic packaging cell line  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications phoenixeco ecotropic packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Phoenixeco Ecotropic Packaging Cell Line, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phoenixtm eco retroviral packaging cell line  (Orbigen Inc)
90
Orbigen Inc phoenixtm eco retroviral packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Phoenixtm Eco Retroviral Packaging Cell Line, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phoenixtm eco retroviral packaging cell line/product/Orbigen Inc
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gp+e86 packaging cell line  (Genetix Pharmaceuticals Inc)
90
Genetix Pharmaceuticals Inc gp+e86 packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Gp+E86 Packaging Cell Line, supplied by Genetix Pharmaceuticals Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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φnx packaging cell line  (Orbigen Inc)
90
Orbigen Inc φnx packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
φnx Packaging Cell Line, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ecopack2–293  (Becton Dickinson)
90
Becton Dickinson ecopack2–293
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Ecopack2–293, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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high pressure’s fruit jelly  (Meidi ya Coporation)
90
Meidi ya Coporation high pressure’s fruit jelly
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
High Pressure’s Fruit Jelly, supplied by Meidi ya Coporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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293t-hek phoenix amphotropic cells  (Orbigen Inc)
90
Orbigen Inc 293t-hek phoenix amphotropic cells
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
293t Hek Phoenix Amphotropic Cells, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retroviral packaging cells, line pa317  (Promega)
90
Promega retroviral packaging cells, line pa317
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Retroviral Packaging Cells, Line Pa317, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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packaging cell line c2  (ICN Biomedicals)
90
ICN Biomedicals packaging cell line c2
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Packaging Cell Line C2, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phoenix-eco 293 packaging cell line  (Orbigen Inc)
90
Orbigen Inc phoenix-eco 293 packaging cell line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Phoenix Eco 293 Packaging Cell Line, supplied by Orbigen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistics package ldp-line  (CH Instruments)
90
CH Instruments statistics package ldp-line
Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a <t>retroviral</t> vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.
Statistics Package Ldp Line, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a retroviral vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.

Journal: Virology

Article Title: Characterization of the Epstein-Barr virus glycoprotein BMRF-2.

doi: 10.1016/j.virol.2006.09.047

Figure Lengend Snippet: Fig. 8. Glycosylation of BMRF-2. (A) HSC3sort cells were transduced with a retroviral vector expressing BMRF-2-GFP. After 48 h, cells were fixed and co- immunostained for BMRF-2 with ER or Golgi markers. ER and Golgi were detected with staining for GRP94 and Rhodamine-Lens Culinaris Agglutinin, respectively. BMRF-2 is shown in green fluorescence as a GFP fusion protein and the ER and Golgi markers are shown in red fluorescence. Yellow indicates colocalization of BMRF-2 with ER or Golgi markers. (B) The 293T cells were transfected with the BMRF-2 gene for 48 h and the membrane fractions of these cells were isolated. In a parallel experiment, total proteins from purified EBV virions were extracted. Transfected cells and virion samples were treated with a combination of neuraminidase and O-glycosidase, and with O-glycosidase and neuraminidase independently. Untreated samples served as a control. Treated and untreated samples were subjected to Western blot analysis using rat anti- BMRF-2 immune serum and proteins were separated in 7 M urea gels. Neu, neuraminidase; OG, O-glycosydase. Cells, 293T cells transfected with BMRFQ2. Virions, gradient-purified EBV virions.

Article Snippet: A human embryonic kidney cell line (293T) and a retroviral packaging cell line (ProPak A.52) were purchased from American Type Culture Collection (ATCC,Manassas, Virginia).

Techniques: Glycoproteomics, Transduction, Retroviral, Plasmid Preparation, Expressing, Staining, Fluorescence, Transfection, Membrane, Isolation, Purification, Control, Western Blot