Journal: ACS chemical neuroscience

Article Title: Small molecules targeting PTPσ—Trk interactions promote sympathetic nerve regeneration

doi: 10.1021/acschemneuro.1c00854

Figure Lengend Snippet: Representative western blots (left) probing for PTPσ and TrkA following TrkA-RFP immunoprecipitation. HEK-293t cells were transfected with TrkA-RFP and PTPσ, treated with either vehicle (DMSO), (A) HJ-01, (B) HJ-02, or (C) HJ-03 then stimulated with CSPGs and NGF. Quantification (right) of PTPσ that co-immunoprecipitated with TrkA-RFP normalized to vehicle treated cells. HJ-01 and HJ-02 reduced the amount of PTPσ that bound to TrkA-RFP at 1μM, but HJ-03 had no effect. (D) HEK-293t cells were transfected with TrkA-RFP and p75NTR, treated with vehicle (DMSO) and HJ-02, then stimulated with CSPGs and NGF. Quantification (right) of p75NTR that co-immunoprecipitated with TrkA-RFP normalized to vehicle treated cells. Data are mean ± SD, n=5-9 experiments; n.s.- not significant, *p<.05, **p<.01 vs. vehicle treated control, one-way ANOVA (Dunnett multiple comparisons post-test).

Article Snippet: We would like to thank Dr. Moses Chao (Skirball Institute, NYU) for the HEK-TrkB cells and for the TrkA-RFP and p75NTR plasmids (Addgene plasmid # 24093, 24091).

Techniques: Western Blot, Immunoprecipitation, Transfection

Journal: Purinergic Signalling

Article Title: Up-regulation of P2X7 receptors mediating proliferation of Schwann cells after sciatic nerve injury

doi: 10.1007/s11302-015-9445-8

Figure Lengend Snippet: Expression of P2X7R ir in longitudinal sections of normal sciatic nerves. a–c show co-localization of P2X7R ir (red) and S100Aβ ir (green). Note an arrowhead showing a fibre-like structure and an arrow showing a trapezoid structure in a and b; c is the merged image of a and b. Note an arrow indicating a trapezoid structure double labelled by both P2X7R and S100β antibodies (yellow). d–f show co-localization of P2X7R ir (red) and Tuj-1 ir (green). Note an arrow showing a trapezoid structure in d and an arrow showing an axon in e. f is the merged image of d and f; note an arrow indicating a green axon passing through the middle of five trapezoid structures. g–i show co-localization of P2X7R ir (red) and p75NTR ir (green). Note an arrow showing a fibre-like structure in g and h. i is the merged image of g and h. An arrow indicates a double-labelled non-myelinating Schwann cell with P2X7R and p75NTR antibodies (yellow). j–l show co-localization of P2X7R ir (red) and MBP ir (green). Note an arrow showing a fibre-like structure with P2X7R ir, which was not labelled by MBP in l. m–o show co-localization of P2X7R ir (red) and CASPR ir (green). Note that no colocalization of P2X7R ir and CASPR ir was observed. Scale bars in a–c and g–i = 100 μm; scale bars in d–f = 50 μm

Article Snippet: The sections were washed 3–5 min in PBS, and then preincubated in a blocking solution (10 % normal bovine serum, 0.2 % Triton X-100, 0.4 % sodium azide in 0.01 mol/L PBS, pH 7.2) for 30 min followed by incubation with the primary antibodies: P2X7R (1:1000), Alomone, rabbit polyclonal, APR-004; S100β (1:400), Tuj-1 (1:200), p75NTR, PCNA (1:200), MBP (1:200), mouse monoclonal antibodies from Boster, CASPR (1:400) mouse monoclonal antibodies from Santa Cruz, at room temperature overnight.

Techniques: Expressing

Journal: Cell Stem Cell

Article Title: Selective advantage of mutant stem cells in human clonal hematopoiesis is associated with attenuated response to inflammation and aging

doi: 10.1016/j.stem.2024.05.010

Figure Lengend Snippet:

Article Snippet: APC anti-human CD271 (clone ME20.4-1.H4) , Miltenyi , Cat# 130-113-418; RRID: AB_2733363.

Techniques: Recombinant, Reverse Transcription, DNA Library Preparation, DNA Sequencing, RNA Sequencing, Purification, Sequencing, Software, Sterility