Journal: Theranostics

Article Title: Major vault protein (MVP) negatively regulates osteoclastogenesis via calcineurin-NFATc1 pathway inhibition

doi: 10.7150/thno.58468

Figure Lengend Snippet: Specific deletion of MVP in osteoclast precursors also induces an osteoporosis phenotype in mice. (A) Image of femurs in Lyz2-Cre (Cre), Mvp f/f (f/f) and Mvp f/f Lyz2-Cre (f/f/△) mice aged 3, 8, and 24 weeks, as assessed by micro-CT. Scale bar: 1 mm. (B) 3D microstructural analysis of femurs from 8 and 24-week-old mice. Scale bar: 500 µm. (C) Quantitative analysis of femurs in (B); BV/TV, bone volume/tissue volume; Tb.Th, trabecular thickness; Tb.N, trabecular number; Tb.Sp, trabecular separation; Ct.Th, cortical thickness. (D) H&E staining of femurs from f/f and f/f/△ mice aged 8 weeks. Scale bar: 400 µm. (E) Quantification of H&E staining in (D). (F) Mineral apposition marked by calcitriol and alizarin red in two groups. Scale bar: 5 µm. (G) Quantitative analysis of relative MAR in (F). MAR, mineral apposition rate. (H) ELISA detection of PINP (serum bone-formation marker) from 8-week-old mice serum. (I) ELISA detection of CTX-1 (serum bone-resorption marker) from 8-week-old mice serum. (J) TRAP staining of femurs from 8-week-old mice. Scale bar: 50 µm. (K) Quantitative analysis of osteoclasts in (J). Oc.S/BS, osteoclast surface/bone surface; N.Oc/B.Pm, osteoclast number/bone perimeter. All experiments were repeated three times. *p < 0.05; **p < 0.01; ns, not significant, as determined by Student's t-test.

Article Snippet: Rates of bone resorption and formation in the serum of eight-week-old mice were determined using C-telopeptide of type I collagen (CTX-1) ELISA (Cusabio) and Pro-collagen I alpha (P1NP) ELISA kit (Immunoway), on accordance with manufacturer instructions.

Techniques: Micro-CT, Staining, Enzyme-linked Immunosorbent Assay, Marker

Journal: Biochemical pharmacology

Article Title: The osteogenic effect of liraglutide involves enhanced mitochondrial biogenesis in osteoblasts

doi: 10.1016/j.bcp.2019.03.024

Figure Lengend Snippet: (A) Differentiation of RCO was assessed by ALP assay. (B) The osteogenic marker, P1NP was assessed from the serum of indicated groups by ELISA. (C) Dynamic histomorphometry for femur diaphysis and (D) femur metaphysis. Data are expressed as mean ± SEM (n = 6/group); *P < 0.05, **P < 0.01 and ***P < 0.001 versus sham.

Article Snippet: ELISA kits for rat procollagen type 1 N-terminal peptide (P1NP) was from My BioSource Inc. (San Diego, CA, USA).

Techniques: ALP Assay, Marker, Enzyme-linked Immunosorbent Assay

Journal: Nutrients

Article Title: Low Serum 25-hydroxyvitamin D Level Does Not Adversely Affect Bone Turnover in Prepubertal Children

doi: 10.3390/nu13103324

Figure Lengend Snippet: General characteristics of study subjects.

Article Snippet: It is important that we measured two bone turnover markers, P1NP and CTX-I, recommended as reference markers by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) and the International Osteoporosis Foundation to measure when assessing bone turnover in adults but nowadays frequently applied by several researchers investigating bone metabolism in children [ , , , ].

Techniques:

Journal: Nutrients

Article Title: Low Serum 25-hydroxyvitamin D Level Does Not Adversely Affect Bone Turnover in Prepubertal Children

doi: 10.3390/nu13103324

Figure Lengend Snippet: Participants characteristics by tertiles of serum 25(OH)D.

Article Snippet: It is important that we measured two bone turnover markers, P1NP and CTX-I, recommended as reference markers by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) and the International Osteoporosis Foundation to measure when assessing bone turnover in adults but nowadays frequently applied by several researchers investigating bone metabolism in children [ , , , ].

Techniques:

Journal: Nutrients

Article Title: Low Serum 25-hydroxyvitamin D Level Does Not Adversely Affect Bone Turnover in Prepubertal Children

doi: 10.3390/nu13103324

Figure Lengend Snippet: Correlation analysis among study subjects.

Article Snippet: It is important that we measured two bone turnover markers, P1NP and CTX-I, recommended as reference markers by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) and the International Osteoporosis Foundation to measure when assessing bone turnover in adults but nowadays frequently applied by several researchers investigating bone metabolism in children [ , , , ].

Techniques:

Journal: Nutrients

Article Title: Low Serum 25-hydroxyvitamin D Level Does Not Adversely Affect Bone Turnover in Prepubertal Children

doi: 10.3390/nu13103324

Figure Lengend Snippet: Linear regression analysis for the prediction of bone turnover markers and IGF-1 variation by low 25(OH)D ( <20 ng/mL) in study subjects.

Article Snippet: It is important that we measured two bone turnover markers, P1NP and CTX-I, recommended as reference markers by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) and the International Osteoporosis Foundation to measure when assessing bone turnover in adults but nowadays frequently applied by several researchers investigating bone metabolism in children [ , , , ].

Techniques:

Journal: Scientific Reports

Article Title: Sclerostin Antibody Treatment Increases Bone Formation, Bone Mass, and Bone Strength of Intact Bones in Adult Male Rats

doi: 10.1038/srep15632

Figure Lengend Snippet: Analysis of serum bone turnover markers.

Article Snippet: The serum concentration of bone formation marker procollagen type 1 N-terminal propeptide (P1NP) and bone resorption marker C-telopeptide of type 1 collagen (CTX-1) were measured using commercially available ELISA kits (GenAsia, Shanghai, China).

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Thy-1 Deficiency Augments Bone Loss in Obesity by Affecting Bone Formation and Resorption

doi: 10.3389/fcell.2018.00127

Figure Lengend Snippet: Lack of Thy-1 reduces osteoblast differentiation as well as bone formation and increases osteoclast differentiation and bone resorption in obese mice. Wildtype (WT) and Thy-1 −/− (KO) mice were fed with a high fat diet for 18 weeks (HFD). (A–J) Bone formation and (K-O) bone resorption were analyzed. The (A) osteoblast number per bone perimeter (Ob.N/B.Pm), (B) osteoblast surface per bone surface (Ob.S/BS) were analyzed using histology methods. Gene expression of the osteogenic markers (C) runt-related transcription factor 2 ( Runx2 ), (D) and alkaline phosphatase ( Tnalp ) was analyzed by RT-PCR technique. (E) The serum concentration of total procollagen type 1 amino-terminal propeptide (P1NP) was analyzed using ELISA technique and the (F) osteoid surface per bone perimeter (Osteid.S/B.Pm) by histology. (G) Representative sections of von Kossa/van Gieson staining of bone (black) and cartilage (dense red area close to the bone). (H) The bone formation rate per bone surface (BFR/BS) as well as (I) mineral apposition rate (MAR) were determined by histomorphometric analysis (double calcein labeling). (J) Representative images of calcein labeling (green). (K) Osteoclast number per bone perimeter (Oc.N/B.Pm) and (L) osteoclast surface per bone surface (Oc.S/BS) were analyzed by staining of tartrate resistant acid phosphatase (TRAP). (M) Representative images of TRAP staining (red spots and black arrows = TRAP-positive cell/osteoclast). (N) Serum concentration of the bone resorption marker carboxy-terminal collagen crosslinks (CTX) was measured by ELISA technique. (O) Gene expression of Trap was evaluated via RT-PCR. Each point represents one mouse and median ± SD is presented. Asterisks denote significance level of ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 (Student’s t -test).

Article Snippet: Serum levels of type 1 procollagen amino-terminal propeptide (P1NP), C-terminal telopeptide (CTX and P1NP: Immundiagnostik Systems, Germany), sclerostin (ALPCO, serum dilution 1:5), and Dkk-1 (R&D) were detected using immunoassay kits according to the manufacturer’s protocols.

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Enzyme-linked Immunosorbent Assay, Staining, Labeling, Marker

Journal: Biomedicines

Article Title: High-Dose Intravenous Steroid Treatment Seems to Have No Long-Term Negative Effect on Bone Mineral Density of Young and Newly Diagnosed Multiple Sclerosis Patients: A Pilot Study

doi: 10.3390/biomedicines11020603

Figure Lengend Snippet: Patients’ characteristics at baseline and after 24-months follow-up.

Article Snippet: N-terminal-propeptide-procollagen-type-1 (P1NP) was measured by P1NP ELISA kit, Abbexa Ltd., with intra- and inter-assay CVs < 10%.

Techniques:

Journal: European Journal of Applied Physiology

Article Title: Caloric restriction induces anabolic resistance to resistance exercise

doi: 10.1007/s00421-020-04354-0

Figure Lengend Snippet: Change in P1NP by condition and between time points adjusted to control for order effects ( n = 7). †Indicates p < 0.10; *indicates p < 0.05

Article Snippet: Commercially available assays were used to measure serum concentrations of IGF-1 [R&D Systems], GH [R&D Systems], P1NP [ABClonal], and sclerostin [Biomedica].

Techniques: