Journal: The Prostate

Article Title: A CD24-p53 Axis Contributes to African-American Prostate Cancer Disparities

doi: 10.1002/pros.23973

Figure Lengend Snippet: (A) Representative IHC data showed no CD24 staining in normal prostate (left panel) or benign prostatic hyperplasia (BPH, right panel). (B) Representative IHC data showed CD24 staining of prostate cancer cells with no staining of adjacent normal prostate epithelial cells. The red arrows indicate adjacent normal prostate epithelial cells. (C) CD24, mutp53, MDM2, and ARF staining of representative UAB prostate cancer samples. Representative IHC data showed co-expression of CD24 and mutant p53 from the same case. (D) CD24 and mutp53 IHC staining of two representative PCaP TMA prostate cancer samples. mutp53, mutant p53 protein. All experiments were repeated three times.

Article Snippet: Antibodies were human CD24 (ML5, BD Biosciences), p53 (DO-1, Santa Cruz Biotechnology), MDM2 (SMP14, BD Biosciences), and ARF (also p14-ARF, E3X6D, Cell Signaling).

Techniques: Staining, Expressing, Mutagenesis, Immunohistochemistry

Journal: The Prostate

Article Title: A CD24-p53 Axis Contributes to African-American Prostate Cancer Disparities

doi: 10.1002/pros.23973

Figure Lengend Snippet: Ethnic/racial distribution comparisons in gene expressions

Article Snippet: Antibodies were human CD24 (ML5, BD Biosciences), p53 (DO-1, Santa Cruz Biotechnology), MDM2 (SMP14, BD Biosciences), and ARF (also p14-ARF, E3X6D, Cell Signaling).

Techniques: Significance Assay

Journal: The Prostate

Article Title: A CD24-p53 Axis Contributes to African-American Prostate Cancer Disparities

doi: 10.1002/pros.23973

Figure Lengend Snippet: (A-C) Pearson correlations of the H-scores of CD24 relative to mutp53, MDM2, and ARF staining in UAB prostate cancer samples. (D-G) Protein expression levels of CD24, mutp53, MDM2, and ARF for AAs and EAs in the UAB prostate cancers. mutp53, mutant p53 protein; AA, African-American; EA, European-American.

Article Snippet: Antibodies were human CD24 (ML5, BD Biosciences), p53 (DO-1, Santa Cruz Biotechnology), MDM2 (SMP14, BD Biosciences), and ARF (also p14-ARF, E3X6D, Cell Signaling).

Techniques: Staining, Expressing, Mutagenesis

Journal: Cancers

Article Title: CDKN2A -Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome

doi: 10.3390/cancers13205139

Figure Lengend Snippet: IHC/IF staining conditions for antibodies used in this study.

Article Snippet: P14 , mouse , Cell Signaling , 2407S , ST Citrate , 1:50.

Techniques: Staining

Journal: BioMed Research International

Article Title: Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits

doi: 10.1155/2017/2089645

Figure Lengend Snippet: Primary and secondary antibodies and normal sera used in the present study.

Article Snippet: Rabbit polyclonal IgG anti-aromatase , 1 : 500 , NB-200-1596 , Novus Biologicals.

Techniques:

Journal: BioMed Research International

Article Title: Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits

doi: 10.1155/2017/2089645

Figure Lengend Snippet: Serum concentrations of total estradiol (E2, (a)) and testosterone (T, (b)) vary between the C, OVX, and OVX + EB groups. (c) The ratio of E2 to T (as logarithm) was calculated to estimate the extent of extragonadal aromatization. Data are the mean ± SEM ( n = 6 per group). (d) Aromatase expression in the ovary (O) and vagina (V) for control rabbits; Ponceau's Red staining was used to corroborate equal amounts of protein were loaded. Paravaginal neurons from the C (e), OVX (f), and OVX + EB (g) groups express aromatase as showed by immunohistochemistry. (h) Percentages of aromatase-ir neurons are means ± SEM ( n = 6 per group). One-way ANOVA followed by Newman–Keuls post hoc tests were carried out to determine significant differences between groups. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 (compared to the C group); & P < 0.001 (compared to the OVX group). −n, negative neurons; +n, positive neurons; +SGC, positive satellite glial cell. Bar, 20 µ m.

Article Snippet: Rabbit polyclonal IgG anti-aromatase , 1 : 500 , NB-200-1596 , Novus Biologicals.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Oncotarget

Article Title: Androgen-induced alterations in endometrial proteins crucial in recurrent miscarriages

doi: 10.18632/oncotarget.24821

Figure Lengend Snippet: Differentially expressed proteins in endometrial epithelial cells identified from iTRAQ analysis

Article Snippet: For knockdown experiments, siRNA targeting the CDKN2a (sc-37622) gene (100 pmol/well) and siRNA negative control (scrambled, sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Multiplex sample analysis, Sequencing

Journal: Oncotarget

Article Title: Androgen-induced alterations in endometrial proteins crucial in recurrent miscarriages

doi: 10.18632/oncotarget.24821

Figure Lengend Snippet: Up-stream regulator identified from iTRAQ proteomics analysis

Article Snippet: For knockdown experiments, siRNA targeting the CDKN2a (sc-37622) gene (100 pmol/well) and siRNA negative control (scrambled, sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Multiplex sample analysis, Activation Assay

Journal: Oncotarget

Article Title: Androgen-induced alterations in endometrial proteins crucial in recurrent miscarriages

doi: 10.18632/oncotarget.24821

Figure Lengend Snippet: ( A ) CDKN2a ( B ) EPCR and ( C ) ARVCF. Data are present as mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, Student t -test.

Article Snippet: For knockdown experiments, siRNA targeting the CDKN2a (sc-37622) gene (100 pmol/well) and siRNA negative control (scrambled, sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques:

Journal: Oncotarget

Article Title: Androgen-induced alterations in endometrial proteins crucial in recurrent miscarriages

doi: 10.18632/oncotarget.24821

Figure Lengend Snippet: ( A ) CDKN2a, ( B ) EPCR and ( C ) ARVCF. Data are present as mean ± SD ( n = 3). * p < 0.05, Student t -test.

Article Snippet: For knockdown experiments, siRNA targeting the CDKN2a (sc-37622) gene (100 pmol/well) and siRNA negative control (scrambled, sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques:

Journal: Oncotarget

Article Title: Androgen-induced alterations in endometrial proteins crucial in recurrent miscarriages

doi: 10.18632/oncotarget.24821

Figure Lengend Snippet: Treating IK cells with CDKN2a siRNA significantly reduced the expression level of CDKN2a ( A–B ). A 2 (10 −7 M) significantly decreased cell migration ( C–D ), invasion ( E–F ), proliferation ( G–H ) and Jar spheroids attachment ( I–J ) to IK cell monolayer. The data are presented as mean ± SD, * p < 0.05, ** p < 0.01, Student t- test.

Article Snippet: For knockdown experiments, siRNA targeting the CDKN2a (sc-37622) gene (100 pmol/well) and siRNA negative control (scrambled, sc-37007) were purchased from Santa Cruz Biotechnology.

Techniques: Expressing, Migration

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Integrated genomic analyses in PDX model reveal a cyclin-dependent kinase inhibitor Palbociclib as a novel candidate drug for nasopharyngeal carcinoma

doi: 10.1186/s13046-018-0873-5

Figure Lengend Snippet: Genetic alterations in NPC-PDX tumors. ( a ) Copy number variations (CNV) of NPC-PDX tumors versus corresponding patient’s peripheral blood mononuclear cells (PBMC). Genome-wide CNV alterations in four paired PDX tumor samples (ST, LN, LG and LV). CCND1 CNV gain (red arrow) and CDKN2A CNV loss (green arrow) are indicated. ( b ) HE and EB virus-encoded small RNA (EBER) staining of parental NPC tumor with bone metastasis and its derived NPC-PDX. ( c ) CNV profile comparisons of NPC FFPE-Bone and PDX-Bone based on WES and ( d ) ultra-deep sequencing of cancer panel-409 are shown (genes associated with or without copy number alteration are indicated in different colors or in grey, respectively). Observed copy number for each evaluated position is shown on the y-axis as a log 2 scale. Correlation plots with Pearson’s correlation coefficient, r, indicating similarities between two CNV profiles

Article Snippet: Antibodies used in this study: RB1 (CusaBio PA003948), RB-P (Cell Signaling 9307), E2F1 (Santa Cruz SC-193), CDK2 (CusaBio PA001533), CDK4 (Santa Cruz SC-23896), CDK6 (Santa Cruz SC-53638), CCND1 (Santa Cruz SC-8396), CCNE2 (Proteintech 11,935–1-AP), CDKN2A (Prosci 4211), CDKN1A (Santa Cruz SC-6246), PCNA (Proteintech 10,205–2-AP) and GAPDH (Santa Cruz FL-335).

Techniques: Genome Wide, Virus, Staining, Derivative Assay, Sequencing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Integrated genomic analyses in PDX model reveal a cyclin-dependent kinase inhibitor Palbociclib as a novel candidate drug for nasopharyngeal carcinoma

doi: 10.1186/s13046-018-0873-5

Figure Lengend Snippet: CCND1 mRNA expression and IHC staining in NPC patients and PDX tumors. ( a ) The expression fold change of candidate genes ( CCND1, CDKN2A and CDKN2B ) are indicated based on the cDNA microarray data of five PDX tissues, and C666–1 (EBV-positive NPC cells) and NP69 (immortalized normal nasopharyngeal cells, as control) cell lines. ( b ) Agarose gel electrophoresis of RT-PCR products of CCND1 in PBMC, two NPC cell lines and five PDXs (GAPDH serves as an internal control). Cyclin D1 IHC staining in ( c ) NPC no.13 patient, with NPC primary site, NPC metastatic to bone, and PDX-Bone tumor and ( d ) NPC no.2 patient, with NPC metastatic to lymph node, and PDX-LN tumor

Article Snippet: Antibodies used in this study: RB1 (CusaBio PA003948), RB-P (Cell Signaling 9307), E2F1 (Santa Cruz SC-193), CDK2 (CusaBio PA001533), CDK4 (Santa Cruz SC-23896), CDK6 (Santa Cruz SC-53638), CCND1 (Santa Cruz SC-8396), CCNE2 (Proteintech 11,935–1-AP), CDKN2A (Prosci 4211), CDKN1A (Santa Cruz SC-6246), PCNA (Proteintech 10,205–2-AP) and GAPDH (Santa Cruz FL-335).

Techniques: Expressing, Immunohistochemistry, Microarray, Control, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Integrated genomic analyses in PDX model reveal a cyclin-dependent kinase inhibitor Palbociclib as a novel candidate drug for nasopharyngeal carcinoma

doi: 10.1186/s13046-018-0873-5

Figure Lengend Snippet: Correlation of CNV of CCND1, CDKN2A and RAD52 with high EBV copy number in 22 NPC plasma. ( a ) CNV of CCND1 , CDKN2A and RAD52 in 22 NPC plasma with high EBV DNA load (> 5000 copies/ml) based on Q-PCR results. ( b ) Correlation plot between CNV of CCND1 , CDKN2A and RAD52 and log EBV DNA load in 22 NPC plasma samples ( c ) Correlation between CNV of the CCND1 / CDKN2A ratio and log EBV load in 22 NPC plasma. Pearson’s correlation coefficient, r, and equations of regression are indicated

Article Snippet: Antibodies used in this study: RB1 (CusaBio PA003948), RB-P (Cell Signaling 9307), E2F1 (Santa Cruz SC-193), CDK2 (CusaBio PA001533), CDK4 (Santa Cruz SC-23896), CDK6 (Santa Cruz SC-53638), CCND1 (Santa Cruz SC-8396), CCNE2 (Proteintech 11,935–1-AP), CDKN2A (Prosci 4211), CDKN1A (Santa Cruz SC-6246), PCNA (Proteintech 10,205–2-AP) and GAPDH (Santa Cruz FL-335).

Techniques: Clinical Proteomics