Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-human/mouse pS6 [S235/S236] (175Lu) clone N7-548 , Fluidigm , 3175009A.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry

Journal: Cell metabolism

Article Title: Multiplexed in Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes

doi: 10.1016/j.cmet.2019.01.003

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 172Yb-pS6 (Clone N7–548) , Fluidigm , Cat#3172008A.

Techniques: Software

Journal: PLoS ONE

Article Title: The PI3-Kinase/mTOR-Targeting Drug NVP-BEZ235 Inhibits Growth and IgE-Dependent Activation of Human Mast Cells and Basophils

doi: 10.1371/journal.pone.0029925

Figure Lengend Snippet: A: HMC-1.1 cells (upper panel), HMC-1.2 cells (middle panel), and KU812 cells (lower panel) were incubated with control medium (Co) or medium containing NVP-BEZ235 (1 or 10 µM) or RAD001 (1 or 10 µM) at 37°C for 4 hours. Thereafter, cells were permeabilized and stained with antibodies against pAkt (S473), pS6, or pSTAT5 as described in the text. Expression of phosphorylated (p) signaling molecules in HMC-1 cells and KU812 cells were determined by flow cytometry. Results show the staining index (mean fluorescence intensity values corrected for the isotype) and represent the mean±S.D. from three independent experiments. B: HMC-1.1 cells (upper panel), HMC-1.2 cells (middle panel), and KU812 cells (lower panel) were cultured in the absence or presence of NVP-BEZ235 (1 µM) RAD001 (1 µM) at 37°C for 4 hours. Thereafter, cells were lysed and Western blotting was performed using antibodies against pAkt (S473), Akt, pSTAT5, STAT5, pS6 and β-Actin. C: Western blot results were quantified by densitometry in HMC-1.1 cells (upper panel), HMC-1.2 cells (middle panel), and KU812 cells (lower panel). Results represent the mean±S.D. from three independent experiments.

Article Snippet: For staining of cytoplasmic molecules, KU812 and HMC-1 cells were permeabilized by methanol (−20°C, 15 minutes) and then incubated with mAb against phosphorylated Akt (pAkt, S473) (M89-61), pS6 (N7-548), pSTAT5 (clone 47), or activated caspase 3 (C92-605) (all from Becton Dickinson Biosciences) for 30 minutes.

Techniques: Incubation, Staining, Expressing, Flow Cytometry, Fluorescence, Cell Culture, Western Blot

Journal: Immunity

Article Title: Loss of CD4 + T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection

doi: 10.1016/j.immuni.2023.07.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: They were then stained with Alexa 647 anti-pS6, washed 3 times and were assessed by flow cytometry on a BD FACS CANTO (BD biosciences).

Techniques: Virus, Isolation, Recombinant, Staining, Cell Isolation, Flow Cytometry, RNA Sequencing Assay, In Vitro, Microarray, CRISPR, Software

Journal: Journal of Geophysical Research. Space Physics

Article Title: GOES‐R Series X‐Ray Sensor (XRS): 1. Design and Pre‐Flight Calibration

doi: 10.1029/2024JA032925

Figure Lengend Snippet: GOES‐R X‐Ray Sensor (XRS) Design Specifications

Article Snippet: The A2 and B2 detectors are quadrant photodiodes (OptoDiode AXUV‐PS6) instead of single element photodiodes (OptoDiode AXUV‐100) as used for A1 and B1.

Techniques:

Journal: Journal of Geophysical Research. Space Physics

Article Title: GOES‐R Series X‐Ray Sensor (XRS): 1. Design and Pre‐Flight Calibration

doi: 10.1029/2024JA032925

Figure Lengend Snippet: GOES‐R‐Series X‐Ray Sensor Calibration Results

Article Snippet: The A2 and B2 detectors are quadrant photodiodes (OptoDiode AXUV‐PS6) instead of single element photodiodes (OptoDiode AXUV‐100) as used for A1 and B1.

Techniques:

Journal: Journal of Geophysical Research. Space Physics

Article Title: GOES‐R Series X‐Ray Sensor (XRS): 1. Design and Pre‐Flight Calibration

doi: 10.1029/2024JA032925

Figure Lengend Snippet: Field of view (FOV) maps for GOES‐18 XRS‐B1 are in panels A and C, and FOV maps for GOES‐18 XRS‐B2 are in panels B and D. Top panels are maps from prelaunch calibration, and the bottom panels were from an inflight FOV map obtained on 28 November 2023. The bottom FOV maps are over ±15 arcmin (0.25°), and the flight map range of ±15 arcmin is represented as a solid‐black box in the top FOV maps that are over ±45 arcmin (0.75°).

Article Snippet: The A2 and B2 detectors are quadrant photodiodes (OptoDiode AXUV‐PS6) instead of single element photodiodes (OptoDiode AXUV‐100) as used for A1 and B1.

Techniques:

Journal: Journal of Geophysical Research. Space Physics

Article Title: GOES‐R Series X‐Ray Sensor (XRS): 1. Design and Pre‐Flight Calibration

doi: 10.1029/2024JA032925

Figure Lengend Snippet: Model‐fit results for all X‐Ray Sensor (XRS) diodes background signal for the GOES‐U (GOES‐19) XRS unit. These results are typical for the other XRS flight models (FM). The Dark diodes are D1 and D2. AXUV‐100 diodes are used for XRS A1 and B1. AXUV‐PS6 quadrant diodes are used for A2 and B2, with labels for its four diodes as X21, X22, X23, and X24 (where X is A or B).

Article Snippet: The A2 and B2 detectors are quadrant photodiodes (OptoDiode AXUV‐PS6) instead of single element photodiodes (OptoDiode AXUV‐100) as used for A1 and B1.

Techniques:

Journal: Journal of Geophysical Research. Space Physics

Article Title: GOES‐R Series X‐Ray Sensor (XRS): 1. Design and Pre‐Flight Calibration

doi: 10.1029/2024JA032925

Figure Lengend Snippet: The GOES‐17 XRS‐B2 linearity calibration at Synchrotron Ultraviolet Radiation Facility (SURF). (left) Beam current (BC)‐normalized signal (summed over all quadrants) versus SURF BC reveals a flat level independent of SURF BC. Blue diamonds represent individual data points, and red squares show the average values for measurements taken at different BC levels. The rollover at high BC is due to electrometer saturation. (right) Deviations from the mean normalized signal is also very flat, with ±1% dotted lines and ±2% dashed lines shown for reference.

Article Snippet: The A2 and B2 detectors are quadrant photodiodes (OptoDiode AXUV‐PS6) instead of single element photodiodes (OptoDiode AXUV‐100) as used for A1 and B1.

Techniques:

Journal: Journal of Geophysical Research. Space Physics

Article Title: GOES‐R Series X‐Ray Sensor (XRS): 1. Design and Pre‐Flight Calibration

doi: 10.1029/2024JA032925

Figure Lengend Snippet: Example of in‐flight X‐Ray Sensor (XRS) signals for GOES‐16 XRS A2 and B2 on day 2017/249 (6 September 2017) when there was a large X10 flare near 12 UT. These XRS signals are first corrected for the background signal, which is a bigger effect during non‐flaring times for A2 than B2. The estimated signals for the Astrophysical Plasma Emission Code solar minimum and maximum reference spectra are the horizontal dashed lines (blue for A2 and red for B2).

Article Snippet: The A2 and B2 detectors are quadrant photodiodes (OptoDiode AXUV‐PS6) instead of single element photodiodes (OptoDiode AXUV‐100) as used for A1 and B1.

Techniques: