p-rip1(ser166) Search Results


prip1 cell signaling  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc prip1 cell signaling
Prip1 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prip1 cell signaling/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
prip1 cell signaling - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
p rip1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc p rip1
P Rip1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p rip1/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
p rip1 - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
anti p rip1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti p rip1
Anti P Rip1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p rip1/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
anti p rip1 - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
1000 p rip1 47928t cell signaling technology 1  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc 1000 p rip1 47928t cell signaling technology 1
1000 P Rip1 47928t Cell Signaling Technology 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1000 p rip1 47928t cell signaling technology 1/product/Cell Signaling Technology Inc
Average 92 stars, based on 1 article reviews
1000 p rip1 47928t cell signaling technology 1 - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier
anti p rip1 ser166  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc anti p rip1 ser166
Anti P Rip1 Ser166, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p rip1 ser166/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
anti p rip1 ser166 - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
p-rip1(ser166)  (Affinity Biosciences)
90
Affinity Biosciences p-rip1(ser166)
P Rip1(Ser166), supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p-rip1(ser166)/product/Affinity Biosciences
Average 90 stars, based on 1 article reviews
p-rip1(ser166) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
prip1-ser166 antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc prip1-ser166 antibody
Ser320 on RIPK1 is important for its biological function. A, Upper panel: Representative immuno-blot comparing RIPK1 phosphorylation at <t>Ser166</t> in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1, RIPK1-S320A, or RIPK1-K45M. The indicated cell lines were control-treated or treated for 30 min with zVAD and SM prior to 2 h TNFα exposure, the cells lysed, and immuno-blotted with the indicated antibodies. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. B, As in (A), but the indicated cell lines were control-treated or treated for 30 min with zVAD, SM, and TPCA-1 prior to 2 h TNFα exposure. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. C, Left panel: Representative immuno-blots comparing Complex II assembly in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were treated for 30 min with zVAD and SM prior to 4 h. TNFα exposure, the cells lysed, Complex II isolated by immuno-precipitation with a Flag antibody, and the immuno-precipitates immuno-blotted with the indicated antibodies. The cell lysates were immunoblotted with the indicated antibodies to monitor total protein levels. Right Panel: Quantification of the CASPASE-8/RIPK1 and FADD/RIPK1 ratios in Complex II. Data from three independent experiments were used for the quantification. D, Representative dot plots of flow cytometry analysis to compare necrotic cell death in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were control-treated (left) or treated for 30 min with zVAD and SM prior to 24 h TNFα exposure (right), and cell viability monitored by Annexin V and Propidium Iodide (PI) staining followed by flow cytometry. E, Quantification of the double positive stained cells in (D). Data from three independent experiments were used for the quantification. Error bars represent triplicate measurements + s.e.m. Data were analyzed by Student's t test, **p ≤ 0.01, *p ≤ 0.05.
Prip1 Ser166 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prip1-ser166 antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
prip1-ser166 antibody - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
primary antibodies specific for p-rip1 (ser166)  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc primary antibodies specific for p-rip1 (ser166)
Ser320 on RIPK1 is important for its biological function. A, Upper panel: Representative immuno-blot comparing RIPK1 phosphorylation at <t>Ser166</t> in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1, RIPK1-S320A, or RIPK1-K45M. The indicated cell lines were control-treated or treated for 30 min with zVAD and SM prior to 2 h TNFα exposure, the cells lysed, and immuno-blotted with the indicated antibodies. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. B, As in (A), but the indicated cell lines were control-treated or treated for 30 min with zVAD, SM, and TPCA-1 prior to 2 h TNFα exposure. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. C, Left panel: Representative immuno-blots comparing Complex II assembly in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were treated for 30 min with zVAD and SM prior to 4 h. TNFα exposure, the cells lysed, Complex II isolated by immuno-precipitation with a Flag antibody, and the immuno-precipitates immuno-blotted with the indicated antibodies. The cell lysates were immunoblotted with the indicated antibodies to monitor total protein levels. Right Panel: Quantification of the CASPASE-8/RIPK1 and FADD/RIPK1 ratios in Complex II. Data from three independent experiments were used for the quantification. D, Representative dot plots of flow cytometry analysis to compare necrotic cell death in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were control-treated (left) or treated for 30 min with zVAD and SM prior to 24 h TNFα exposure (right), and cell viability monitored by Annexin V and Propidium Iodide (PI) staining followed by flow cytometry. E, Quantification of the double positive stained cells in (D). Data from three independent experiments were used for the quantification. Error bars represent triplicate measurements + s.e.m. Data were analyzed by Student's t test, **p ≤ 0.01, *p ≤ 0.05.
Primary Antibodies Specific For P Rip1 (Ser166), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies specific for p-rip1 (ser166)/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
primary antibodies specific for p-rip1 (ser166) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
antibodies against p-rip3 (ser232, #ta7443)  (Abmart Inc)
90
Abmart Inc antibodies against p-rip3 (ser232, #ta7443)
Ser320 on RIPK1 is important for its biological function. A, Upper panel: Representative immuno-blot comparing RIPK1 phosphorylation at <t>Ser166</t> in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1, RIPK1-S320A, or RIPK1-K45M. The indicated cell lines were control-treated or treated for 30 min with zVAD and SM prior to 2 h TNFα exposure, the cells lysed, and immuno-blotted with the indicated antibodies. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. B, As in (A), but the indicated cell lines were control-treated or treated for 30 min with zVAD, SM, and TPCA-1 prior to 2 h TNFα exposure. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. C, Left panel: Representative immuno-blots comparing Complex II assembly in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were treated for 30 min with zVAD and SM prior to 4 h. TNFα exposure, the cells lysed, Complex II isolated by immuno-precipitation with a Flag antibody, and the immuno-precipitates immuno-blotted with the indicated antibodies. The cell lysates were immunoblotted with the indicated antibodies to monitor total protein levels. Right Panel: Quantification of the CASPASE-8/RIPK1 and FADD/RIPK1 ratios in Complex II. Data from three independent experiments were used for the quantification. D, Representative dot plots of flow cytometry analysis to compare necrotic cell death in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were control-treated (left) or treated for 30 min with zVAD and SM prior to 24 h TNFα exposure (right), and cell viability monitored by Annexin V and Propidium Iodide (PI) staining followed by flow cytometry. E, Quantification of the double positive stained cells in (D). Data from three independent experiments were used for the quantification. Error bars represent triplicate measurements + s.e.m. Data were analyzed by Student's t test, **p ≤ 0.01, *p ≤ 0.05.
Antibodies Against P Rip3 (Ser232, #Ta7443), supplied by Abmart Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against p-rip3 (ser232, #ta7443)/product/Abmart Inc
Average 90 stars, based on 1 article reviews
antibodies against p-rip3 (ser232, #ta7443) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


Ser320 on RIPK1 is important for its biological function. A, Upper panel: Representative immuno-blot comparing RIPK1 phosphorylation at Ser166 in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1, RIPK1-S320A, or RIPK1-K45M. The indicated cell lines were control-treated or treated for 30 min with zVAD and SM prior to 2 h TNFα exposure, the cells lysed, and immuno-blotted with the indicated antibodies. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. B, As in (A), but the indicated cell lines were control-treated or treated for 30 min with zVAD, SM, and TPCA-1 prior to 2 h TNFα exposure. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. C, Left panel: Representative immuno-blots comparing Complex II assembly in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were treated for 30 min with zVAD and SM prior to 4 h. TNFα exposure, the cells lysed, Complex II isolated by immuno-precipitation with a Flag antibody, and the immuno-precipitates immuno-blotted with the indicated antibodies. The cell lysates were immunoblotted with the indicated antibodies to monitor total protein levels. Right Panel: Quantification of the CASPASE-8/RIPK1 and FADD/RIPK1 ratios in Complex II. Data from three independent experiments were used for the quantification. D, Representative dot plots of flow cytometry analysis to compare necrotic cell death in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were control-treated (left) or treated for 30 min with zVAD and SM prior to 24 h TNFα exposure (right), and cell viability monitored by Annexin V and Propidium Iodide (PI) staining followed by flow cytometry. E, Quantification of the double positive stained cells in (D). Data from three independent experiments were used for the quantification. Error bars represent triplicate measurements + s.e.m. Data were analyzed by Student's t test, **p ≤ 0.01, *p ≤ 0.05.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Phospho-proteomic Analysis of TNFα/NFκB Signaling Reveals a Role for RIPK1 Phosphorylation in Suppressing Necrotic Cell Death *

doi: 10.1074/mcp.M117.068189

Figure Lengend Snippet: Ser320 on RIPK1 is important for its biological function. A, Upper panel: Representative immuno-blot comparing RIPK1 phosphorylation at Ser166 in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1, RIPK1-S320A, or RIPK1-K45M. The indicated cell lines were control-treated or treated for 30 min with zVAD and SM prior to 2 h TNFα exposure, the cells lysed, and immuno-blotted with the indicated antibodies. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. B, As in (A), but the indicated cell lines were control-treated or treated for 30 min with zVAD, SM, and TPCA-1 prior to 2 h TNFα exposure. Lower panel: Quantification of the pRIPK1-S166/RIPK1 ratio for the immuno-blot. Data from three independent experiments were used for the quantification. C, Left panel: Representative immuno-blots comparing Complex II assembly in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were treated for 30 min with zVAD and SM prior to 4 h. TNFα exposure, the cells lysed, Complex II isolated by immuno-precipitation with a Flag antibody, and the immuno-precipitates immuno-blotted with the indicated antibodies. The cell lysates were immunoblotted with the indicated antibodies to monitor total protein levels. Right Panel: Quantification of the CASPASE-8/RIPK1 and FADD/RIPK1 ratios in Complex II. Data from three independent experiments were used for the quantification. D, Representative dot plots of flow cytometry analysis to compare necrotic cell death in Jurkat RIPK1−/− cells reconstituted with wild-type RIPK1 or RIPK1-S320A. The indicated cell lines were control-treated (left) or treated for 30 min with zVAD and SM prior to 24 h TNFα exposure (right), and cell viability monitored by Annexin V and Propidium Iodide (PI) staining followed by flow cytometry. E, Quantification of the double positive stained cells in (D). Data from three independent experiments were used for the quantification. Error bars represent triplicate measurements + s.e.m. Data were analyzed by Student's t test, **p ≤ 0.01, *p ≤ 0.05.

Article Snippet: Cell lines stably expressing RIPK1 proteins were selected with 0.5 μg/ml Puromycin. . Antibodies and Reagents The following antibodies were purchased from Cell Signaling (Danvers, MA): IKKβ (#8943), pIKKα/β-Ser176/Ser180 (#2697), NFκB p65 (#8242), pNFκB p65-Ser536 (#3033), cIAP1 (#7065), cIAP2 (#3130), IκBα (#9242), pIκBα-Ser32 (#2859), JUN (#9165), pJUN-Ser63 (#2361), HSP27 (#2402), pHSP27-Ser15 (#2404), RPS6, pRPS6-Ser235/Ser236 (#4858), ATF2 (#9226), pATF2-Thr69/Thr71 (#9225), pRIP1-Ser166 (#65746).

Techniques: Phospho-proteomics, Control, Western Blot, Isolation, Immunoprecipitation, Flow Cytometry, Staining