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Image Search Results
Journal: Reproductive biology
Article Title: A change in the steroid metabolic pathway in human testes showing deteriorated spermatogenesis.
doi: 10.1016/j.repbio.2020.02.008
Figure Lengend Snippet: Fig. 2. Autoradiograms of radioactive steroid meta- bolites. (1) Steroid metabolites from 14C-pregnenolone in human testicular samples after TLC development in benzene:acetone (4:1, v/v) solvent systems. A) High-JS group. B) Low-JS group. (2) Steroid metabolites in spot 2 in first TLC in human testicular samples after second TLC de- velopment in cyclohexane:ethyl acetate (1:1, v/v) solvent system. A) High-JS group. B) Low-JS group. (3) Steroid metabolites in spot 3 in first TLC in human testicular samples of Low-JS group after second TLC development in benzene:ethylaceta- te:acetone (8:2:1, v/v) solvent system. Acetylated steroid metabolites in spot 3 in first TLC in human testicular samples of High-JS group after TLC de- velopment in benzene:acetone (4:1, v/v) solvent system. A) Low-JS group. B) Acetylated steroid metabolites of High-JS group (left lane) and acetylated 5β-pregnan- 3β-ol-20-one (right lane). Asterisk represents acety- lated main metabolites. (4) Steroid metabolites in spot 5 in first TLC of human testicular samples of Low-JS group after second and third TLC development in various solvent systems. A) Third TLC development in cyclohexane:ethyl acetate = 3:7. B) Second TLC development in chloroform:MeOH:H2O = 188:12:1. (5) Steroid metabolites with NADPH for in vitro conversion in first TLC of human testicular sam- ples of High- and Low-JS groups after develop- ment in benzene:acetone (4:1, v/v) solvent system. A) High-JS group. B) Low-JS group. Asterisk re- presents Spot 7-1. P4: progesterone; AD: androstenedione; 17α-P4: 17α- hydroxyprogesterone; T: testosterone; 17a, 20b-P: 17α,20β-hydroxy-4-pregnen-3-one.
Article Snippet: For recrystallisation analysis of the radioactive metabolites on spot 7, 5α-pregnan-3β,
Techniques: Solvent, In Vitro