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Image Search Results
Journal: PLoS ONE
Article Title: Sinomenine Sensitizes Multidrug-Resistant Colon Cancer Cells (Caco-2) to Doxorubicin by Downregulation of MDR-1 Expression
doi: 10.1371/journal.pone.0098560
Figure Lengend Snippet: Then MDR-Caco-2 cells were treated with or without sinomenine (500 µM) and celecoxib (25 µM) for 48 hours. ( A ) immunocytochemistry targeting P65 (green). ( B ) Western blot analysis of sinomenine and celecoxib mediated effect on nuclear translocation of P65 in Caco-2 cells and MDR-Caco-2 cells. Antibody to beta-actin was used to ensure equal loading of protein in each lane. ( C ) The relative band density values in the nuclear expression P65 lanes are described in the bar chat. ( D ) The relative band density values in the cytoplasmic p-IκB-α lanes are described in the bar chat. All the results above are expressed as the means ± SE (n = 3) of three independent experiments. *, P<0.05 and **, P<0.01 compared with MDR-Caco-2 group.
Article Snippet: P-glycoprotein (P-gp) mouse anti-human monoclonal antibody,
Techniques: Immunocytochemistry, Western Blot, Translocation Assay, Expressing