Journal: Ecology and Evolution

Article Title: Copepod reproductive effort and oxidative status as responses to warming in the marine environment

doi: 10.1002/ece3.8594

Figure Lengend Snippet: Study design of field monitoring and experimental setup. Adult Acartia sp. were collected with a 200‐m net, then sorted and picked under a microscope for incubation (experiment: 24‐h acclimation and 48 h in the experiment; field monitoring: 24 h). The eggs and nauplii were counted from the sample, and 30 females were picked for GSH, GSSG, GST, CAT, ORAC, and LPX analyses

Article Snippet: Concerning Oxygen Radical Antioxidant Capacity ORAC Activity Assay (Cell Biolabs), we used the assay kit protocol.

Techniques: Microscopy, Incubation

Journal: Ecology and Evolution

Article Title: Copepod reproductive effort and oxidative status as responses to warming in the marine environment

doi: 10.1002/ece3.8594

Figure Lengend Snippet: Biomarkers of oxidative stress in 9, 13, and 16°C treatments in the experiment: (a) GSH:GSSG ratio, (b) LPX (M cumene hydroperoxide equivalents mg −1 mg protein −1 ), (c) ORAC (M trolox equivalents mg −1 ), (d) CAT activity (mol min −1 mg −1 ), and (e) GST activity (mol min −1 mg −1 ). Mean values ( x ¯ ) are shown above each boxplot. The boxplots show the median (vertical line), interquartile range (IQR, the box), and minimum and maximum within 1.5 × IQR (“whiskers”) and outliers (circle)

Article Snippet: Concerning Oxygen Radical Antioxidant Capacity ORAC Activity Assay (Cell Biolabs), we used the assay kit protocol.

Techniques: Activity Assay

Journal: Ecology and Evolution

Article Title: Copepod reproductive effort and oxidative status as responses to warming in the marine environment

doi: 10.1002/ece3.8594

Figure Lengend Snippet: A list of response variables, fixed effects, and test results of chosen linear mixed models

Article Snippet: Concerning Oxygen Radical Antioxidant Capacity ORAC Activity Assay (Cell Biolabs), we used the assay kit protocol.

Techniques: In Situ

Journal: Ecology and Evolution

Article Title: Copepod reproductive effort and oxidative status as responses to warming in the marine environment

doi: 10.1002/ece3.8594

Figure Lengend Snippet: Biomarkers for oxidative stress and antioxidant defenses during summer 2018 in adult Acartia copepods. (a) GSH:GSSG ratio, (b) LPX (M cumene hydroperoxide equivalents mg −1 mg protein −1 ), (c) ORAC (M trolox equivalents mg −1 ), (d) CAT activity (mol min −1 mg −1 ), and (e) GST activity (mol min −1 mg −1 ). Mean values ( x ¯ ) are shown above each boxplot. The boxplots show the median (vertical line), interquartile range (IQR, the box), and minimum and maximum within 1.5 × IQR (“whiskers”) and outliers (circle)

Article Snippet: Concerning Oxygen Radical Antioxidant Capacity ORAC Activity Assay (Cell Biolabs), we used the assay kit protocol.

Techniques: Activity Assay

Journal: Marine Drugs

Article Title: Diphlorethohydroxycarmalol Attenuates Fine Particulate Matter-Induced Subcellular Skin Dysfunction

doi: 10.3390/md17020095

Figure Lengend Snippet: DPHC protected cells against PM 2.5 -induced damage of macromolecules. ( A ) Cell viability was analyzed using trypan blue assay. ( B ) Confocal microscopy was used to analyze lipid peroxidation after diphenyl-1-pyrenylphosphine (DPPP) staining (5 μM). ( C ) Comet assay was used to evaluate DNA damage. ( D ) Avidin-tetramethylrhodamine isothiocyanate (TRITC) (1:200) bound to 8-oxoguanine in DNA was determined using confocal microscopy. ( E ) Cells lysates were analyzed for H2A.X expression using western blotting with actin as loading control. Protein carbonylation was analyzed using a protein carbonyl ELISA kit both ( F ) in vitro and ( H ) in vivo. ( G ) Lipid peroxidation was determined using 8-isoprostaglandin F2α ELISA kit in vivo. ( I ) The mouse epidermal heights were assessed, and images were obtained from every group. Scale bars, 20 μm. * p < 0.05 compared to control groups; # p < 0.05 compared to PM 2.5 -treated groups.

Article Snippet: The levels of 8-isoprostane in the mouse skin tissue was assayed using the Oxiselect TM 8-iso-prostaglandin F2α ELISA kit (Cell Biolabs, San Diego, CA, USA), according to the manufacturer’s instructions.

Techniques: Confocal Microscopy, Staining, Single Cell Gel Electrophoresis, Avidin-Biotin Assay, Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay, In Vitro, In Vivo