Journal: PeerJ

Article Title: Aspirin inhibits tumor progression and enhances cisplatin sensitivity in epithelial ovarian cancer

doi: 10.7717/peerj.11591

Figure Lengend Snippet: (A) MTT cell viability assays were performed to determine the IC50s of aspirin in A2780, Caov-3 and SK-OV-3 cells over a 48-h culture period. (B) Cell proliferation was detected using EdU cell proliferation assays (bar, 50 µm) after treatment with different concentrations of aspirin (0 µM, 100 µM, and 1,000 µM) in A2780 cells. (C) Quantitative assay of proliferation rate in A2780, Caov-3 and SK-OV-3 cells. (D) Colony formation assays were conducted after cells were cultured with different concentrations of aspirin (0 µM, 25 µM, 100 µM, 500 µM, 1,000 µM, and 25 µM) for 14 days in A2780 cells. (E) Quantitative assay of colony counts in A2780, Caov-3 and SK-OV-3 cells. (F) Cells were treated with different concentrations of aspirin (0 µM, 100 µM, and 1,000 µM) for 48 h in A2780 cells, stained with the Annexin V-FITC/PI staining kit, and analyzed using flow cytometry. (G) Quantitative assay of apoptosis rate in A2780, Caov-3 and SK-OV-3 cells. Data are presented as the mean ± SD values. * p < 0.05.

Article Snippet: The EOC cell lines A2780, Caov-3 and SK-OV-3 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Cell Culture, Staining, Flow Cytometry

Journal: PeerJ

Article Title: Aspirin inhibits tumor progression and enhances cisplatin sensitivity in epithelial ovarian cancer

doi: 10.7717/peerj.11591

Figure Lengend Snippet: (A) The cell proliferation rate was determined using EdU cell proliferation assays after the incubation of A2780 cells with CDDP (20 µM) alone or in combination with aspirin (100 µM) (CDDP + aspirin) for 48 h (bar, 50 µm). (B) Quantitative assay of proliferation rate in A2780 and SK-OV-3 cells. (C) Colony formation assays were conducted to observe the growth of A2780 cells after an incubation with CDDP (5 µM) alone or with aspirin (25 µM) for 48 h. (D) Quantitative assay of colony counts in A2780 and SK-OV-3 cells. (E) The percentage of apoptotic cells was determined using flow cytometry after the treatment of A2780 cells with CDDP (20 µM) alone or with aspirin (100 µM). (F) Quantitative assay of apoptosis rate in A2780 and SK-OV-3 cells. Data are presented as the mean ± SD values. * p < 0.05.

Article Snippet: The EOC cell lines A2780, Caov-3 and SK-OV-3 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Incubation, Flow Cytometry

Journal: PeerJ

Article Title: Aspirin inhibits tumor progression and enhances cisplatin sensitivity in epithelial ovarian cancer

doi: 10.7717/peerj.11591

Figure Lengend Snippet: Orthotopic xenograft mouse models of EOC were established with A2780-Luciferase cells using surgical orthotopic implantation to observe the effects of aspirin in vivo . Aspirin was initially administered on day 9 at a dose of 20 mg/kg by gavage and then every other day thereafter, while CDDP administration was initiated at a dose of 3 mg/kg by intraperitoneal injection on day 17 and then every fourth day thereafter. Tumor growth was observed with an IVIS Lumina II beginning on day 8 and then once per week thereafter to obtain in vivo bioluminescence images. (A) The tumor signals in all mice (control group, CDDP group, and CDDP + aspirin group) at every time point are displayed. (B) The tumor growth curve for each group based on the tumor signals is shown. (C) All mice were euthanized via overdose isoflurane inhalation on day 36 and necropsied for the measurement of tumor diameters with Vernier calipers. (D) Quantitative assay of tumor volume in mice. (E) The expression levels of cleaved caspase 3, caspase 3 and Ki67 in tumor tissues harvested from mice bearing orthotopic EOC xenografts (control group, CDDP group, and CDDP + aspirin group) were assessed using IHC. (F) Quantitative assay for the IHC data of cleaved caspase 3, caspase 3 and Ki67. Data are presented as the mean ± SD values. * p < 0.05.

Article Snippet: The EOC cell lines A2780, Caov-3 and SK-OV-3 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Luciferase, In Vivo, Injection, Control, Expressing