Journal: bioRxiv

Article Title: Implicating candidate genes at GWAS signals by leveraging topologically associating domains

doi: 10.1101/087718

Figure Lengend Snippet: siRNA was used to knock down expression of TNAP (positive control), ARHGAP1 , ACP2 , BET1L and DEAF1 . Relative expression of the osteoblast marker genes OSX , OCN and IBSP suggest that GWAS/TAD hits are not major regulators of bone differentiation in this model. Red bars highlight specificity of each siRNA knockdown. Values represent mean ± standard deviation. Statistical significance relative to the scrambled siRNA control is annotated as: * p ≤ 0.05 and # p ≤ 0.10 using a two-tailed Student’s t -test.

Article Snippet: All experiments were conducted in three temporally separated independent technical replicates from cryopreserved P2 aliquots of a human fetal osteoblast cell line (ATCC hFOB 1.19 CRL-11372).

Techniques: Knockdown, Expressing, Positive Control, Marker, Standard Deviation, Control, Two Tailed Test

Journal: bioRxiv

Article Title: Implicating candidate genes at GWAS signals by leveraging topologically associating domains

doi: 10.1101/087718

Figure Lengend Snippet: Validating two ‘TAD Pathway’ predictions for Bone Mineral Density GWAS hits on hFOB cells. siRNA was used to knock down expression of TNAP, ARHGAP1, ACP2, BET1L and DEAF1 . (A) Knockdown of ACP2 decreases cellular metabolic activity, demonstrated using an MTT assay. (B) ALP staining and quantitation indicates that knockdown of TNAP or ACP2 inhibits performance in an osteoblast differentiation assay. Values represent mean ± standard deviation. Statistical significance relative to the scrambled siRNA control is annotated as: * p ≤ 0.05 and # p ≤ 0.10 using a two-tailed Student’s t -test.

Article Snippet: All experiments were conducted in three temporally separated independent technical replicates from cryopreserved P2 aliquots of a human fetal osteoblast cell line (ATCC hFOB 1.19 CRL-11372).

Techniques: Knockdown, Expressing, Activity Assay, MTT Assay, Staining, Quantitation Assay, Differentiation Assay, Standard Deviation, Control, Two Tailed Test

Journal: Journal of Indian Society of Periodontology

Article Title: Biomarkers in biological fluids in adults with periodontitis and/or obesity: A meta-analysis

doi: 10.4103/jisp.jisp_512_19

Figure Lengend Snippet: Search Strategy from different databases

Article Snippet: 3. Balli et al ., 2013[ ] , Turkey, University clinic , Prospective; Interventional , 20 , 20 , O+P+: 40.56±4.11 O−P+: 39.67±3.87 , Standards of the international world workshop for classification of periodontal disease and conditions[ ] , GCF Chemerin: ELISA, Hangzhou Eastbiopharm (China);IL-6: ELISA, Boster Biological Technology (USA) , O+P + group showed higher GCF levels of chemerin and IL-6, compared to O−P + group ( P <0.05 and P <0.008 respectively) , Not included in meta-analysis (just Spearman’s rank correlation values of inflammatory markers are shown).

Techniques: Biomarker Discovery, Marker

Journal: Journal of biomedical materials research. Part A

Article Title: Role of integrin α 2 β 1 in mediating osteoblastic differentiation on three-dimensional titanium scaffolds with submicron-scale texture

doi: 10.1002/jbm.a.35323

Figure Lengend Snippet: Effects of structural properties of 3D scaffolds combined with surface roughness modification on MG63 maturation. MG63 cells were plated on the PT, acid PT, 3D and acid 3D scaffolds and harvested at confluence on TCPS. A: Cell number, (B) ALP specific activity, (C) OCN, (D) OPG, and (E) VEGF levels were measured. Data represented are the mean ± SE of six independent samples. * refers to a statistically-significant p value below 0.05 versus PT; ^ refers to a statistically significant p value below 0.05 versus acid PT; $ refers to a statistically significant p value below 0.05 versus 3D.

Article Snippet: The conditioned media were collected for the ELISA of the late osteoblastic differentiation marker osteocalcin (OCN) (Human Osteocalcin RIA kit, Biomedical Technologies, Stoughton, MA), osteoprotegerin (OPG), a regulator of osteoclast production and activity, and vascular endothelial growth factor (VEGF), a growth factor involved in vasculogenesis (DY805, Osteoprotegerin, DY293B VEGF Duoset, R&D systems, Minneapolis, MN).

Techniques: Modification, Activity Assay

Journal: Journal of biomedical materials research. Part A

Article Title: Role of integrin α 2 β 1 in mediating osteoblastic differentiation on three-dimensional titanium scaffolds with submicron-scale texture

doi: 10.1002/jbm.a.35323

Figure Lengend Snippet: Effects of structural properties of 3D microstructure combined with surface roughness modification on normal human osteoblast maturation. Normal human osteoblasts were plated and harvested at confluence on TCPS. A: Cell number, (B) ALP specific activity, (C) OCN, (D) OPG, and (E) VEGF levels were measured. Data represented are the mean ± SE of six independent samples. * refers to a statistically significant p value below 0.05 versus PT; ^ refers to a statistically significant p value below 0.05 versus acid PT.

Article Snippet: The conditioned media were collected for the ELISA of the late osteoblastic differentiation marker osteocalcin (OCN) (Human Osteocalcin RIA kit, Biomedical Technologies, Stoughton, MA), osteoprotegerin (OPG), a regulator of osteoclast production and activity, and vascular endothelial growth factor (VEGF), a growth factor involved in vasculogenesis (DY805, Osteoprotegerin, DY293B VEGF Duoset, R&D systems, Minneapolis, MN).

Techniques: Modification, Activity Assay

Journal: Journal of biomedical materials research. Part A

Article Title: Role of integrin α 2 β 1 in mediating osteoblastic differentiation on three-dimensional titanium scaffolds with submicron-scale texture

doi: 10.1002/jbm.a.35323

Figure Lengend Snippet: Role of integrin β1 and α2 in osteoblast maturation on 3D scaffolds. MG63 cells were plated and harvested at confluence on TCPS. A: Cell number, (B) ALP specific activity, (C) OCN, (D) OPG, and (E) VEGF levels were measured. Data represented are the mean ± SE of six independent samples. * refers to a statistically significant p value below 0.05 versus PT among the same cell type; ^ refers to a statistically significant p value below 0.05 versus acid PT among the same cell type; # refers to a statistically significant p value below 0.05 versus WT MG63; + refers to a statistically significant p value below 0.05 versus shITGβ1 MG63.

Article Snippet: The conditioned media were collected for the ELISA of the late osteoblastic differentiation marker osteocalcin (OCN) (Human Osteocalcin RIA kit, Biomedical Technologies, Stoughton, MA), osteoprotegerin (OPG), a regulator of osteoclast production and activity, and vascular endothelial growth factor (VEGF), a growth factor involved in vasculogenesis (DY805, Osteoprotegerin, DY293B VEGF Duoset, R&D systems, Minneapolis, MN).

Techniques: Activity Assay

Journal: Journal of biomedical materials research. Part A

Article Title: Role of integrin α 2 β 1 in mediating osteoblastic differentiation on three-dimensional titanium scaffolds with submicron-scale texture

doi: 10.1002/jbm.a.35323

Figure Lengend Snippet: Effects of structural properties of 3D scaffolds on MG63 cells during culture time. MG63 were plated and the day when cells get confluence on TCPS is marked as 0. A: Cell number, (B) ALP specific activity, (C) OCN, (D) OPG, and (E) VEGF levels were measured. Data represented are the mean ± SE of six independent samples. * refers to a statistically significant p value below 0.05 versus TCPS base line; ^ refers to a statistically significant p value below 0.05 versus PT at the same time point.

Article Snippet: The conditioned media were collected for the ELISA of the late osteoblastic differentiation marker osteocalcin (OCN) (Human Osteocalcin RIA kit, Biomedical Technologies, Stoughton, MA), osteoprotegerin (OPG), a regulator of osteoclast production and activity, and vascular endothelial growth factor (VEGF), a growth factor involved in vasculogenesis (DY805, Osteoprotegerin, DY293B VEGF Duoset, R&D systems, Minneapolis, MN).

Techniques: Activity Assay

Journal: The Journal of investigative dermatology

Article Title: Epithelium-derived Wnt ligands are essential for maintenance of underlying digit bone

doi: 10.1016/j.jid.2016.03.018

Figure Lengend Snippet: (a–g) Immunohistochemistory for Osx (a anb b), MITF (c), Tcf1 (d and g), Wls (e) and β-catenin (f) on terminal phalanx and nail epithelium of human toe. (h) Schematic illustration of the distribution pattern of Osx+, MITF+, Tcf1+ and Wls+ cells in mouse digit and human toe. (i) Experimental scheme of co-culture experiment and TopFlash assay on human osteoblasts and osteoclast precursors. (j and k) Representative result of TopFlash assay of human osteoblast and human osteoclast precursors. (l) Experimental scheme of differentiation assay of human osteoclast precursors. (m–p) Differentiation analysis of human osteoclast precursors cultured with no nail matrix (m), nail matrix (n), nail matrix with β-catenin knockdown (o), and nail matrix and IWP-2 inhibitor (p). (q) qPCR analysis of osteoclast differentiation markers using cells at 3 days after induction of osteoclast differentiation.(r and s) Quantification analysis of the area that covered by mature osteoclast (r) and number of osteoclast precursors and mature osteoclasts (s). Lines indicate border between terminal phalanx and dermis. Dashed lines indicate border between epithelium and dermis. Arrowheads indicate cells that are positive for indicated marker. Data are presented as the mean ± SD (n=6). Scale bars, 1mm in (a); 50 µm in (b); 200 µm in (h).

Article Snippet: Human osteoblasts/osteoclast precursors and mouse nail epithelial cell co-culture with TopFlash Assay Human osteoblasts and osteoclast precursors were purchased from PromoCell GmbH (Germany, C-12760) and Lonza (MD, 711.98) and cultured according to the manufacturer’s instruction.

Techniques: Co-Culture Assay, TOPFlash assay, Differentiation Assay, Cell Culture, Marker

Journal: The Journal of investigative dermatology

Article Title: Epithelium-derived Wnt ligands are essential for maintenance of underlying digit bone

doi: 10.1016/j.jid.2016.03.018

Figure Lengend Snippet: In the presence of Wnt ligands derived from the nail epithelium, osteoblasts that form the bone and precursors of osteoclasts that break down the bone both display Wnt pathway activation. Wnt activation in osteoblasts results in osteoprotegerin (Opg) expression, which is known to inhibit osteoclast activation and differentiation. Wnt activation in osteoclast precursors, on the other hand, inhibits their differentiation into mature osteoclasts, together ensuring the bone maintenance.

Article Snippet: Human osteoblasts/osteoclast precursors and mouse nail epithelial cell co-culture with TopFlash Assay Human osteoblasts and osteoclast precursors were purchased from PromoCell GmbH (Germany, C-12760) and Lonza (MD, 711.98) and cultured according to the manufacturer’s instruction.

Techniques: Derivative Assay, Activation Assay, Expressing