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Image Search Results
Journal: Science signaling
Article Title: Integrated in vivo multi-omics analysis identifies p21-activated kinase signaling as a driver of colitis
doi: 10.1126/scisignal.aan3580
Figure Lengend Snippet: Differential regulation of RNA and protein. (A) Scatter plot of fold change (inflamed vs. non-inflamed) in RNA expression plotted against fold change in protein abundance for species that were present in both data sets. Colored dots represent ECM proteins (purple), acute-phase proteins (red), and neutrophil proteins (orange), with the arrowheads indicating genes that were further investigated in panels D and E. (B) Collagen expression in the RNA data and abundance in the protein data. Differential abundance of MMPs and TIMPs in the MS data are indicated in the heatmap inset with a key. (C) Tissue expression patterns of acute phase (red) and neutrophil (orange) transcripts. Each gene was normalized to a maximum of one, and all of the genes from each category averaged to generate bars. (D) Induction of acute phase RNA in the liver during inflammation. Bars represent the ratio of liver expression to colon expression for Orm1 and Fga from N = 2 each of inflamed and non-inflamed animals. Assays were performed in duplicate. * p < 0.05 in an unpaired T test. p = 0.085 for Orm1. (E) Loss of neutrophil gene expression by colonic neutrophils. Abundance of the RNA for Camp and Elane from bone marrow neutrophils relative to that in neutrophils isolated from colon is shown. The plot represents log-transformed data from N = 2 inflamed animals, normalized to the smallest expression value for each gene. For both genes, p < 0.05 in a paired T test. (F) Model depicting RNA expression (R) and protein abundance (P) for acute phase proteins (APP) and neutrophil proteins (represented by a polymorphonuclear cell symbol) in the colon and distant organ sites.
Article Snippet: For liver genes, we assayed Fibronectin alpha chain ( Fga , TaqMan Mm00802584_m1) and Alpha-1-acid glycoprotein 1 ( Orm1 , TaqMan
Techniques: RNA Expression, Expressing, Isolation, Transformation Assay
Journal: Neuroscience Bulletin
Article Title: Comprehensive Proteomic Profiling of Patients’ Tears Identifies Potential Biomarkers for the Traumatic Vegetative State
doi: 10.1007/s12264-018-0259-x
Figure Lengend Snippet: Gene Ontology analysis revealed 21 proteins involved in response to wounding.
Article Snippet: ELISA and Receiver Operating Characteristic (ROC) Curve Analysis In the verification stage, the levels of 7 promising tear proteins [cystatin B (CTSB), protease, serine 1 (PRSS1), S100 calcium-binding protein A7 (S100A7), glutathione S-transferase P (GSTP1), complement factor H (CFH), kininogen 1 (KNG1), and alpha-1-acid glycoprotein 1 (ORM1)] were measured using the ELISA kits for human CSTB, human PRSS1, human CFH, human KNG1, and
Techniques:
Journal: Neuroscience Bulletin
Article Title: Comprehensive Proteomic Profiling of Patients’ Tears Identifies Potential Biomarkers for the Traumatic Vegetative State
doi: 10.1007/s12264-018-0259-x
Figure Lengend Snippet: Levels of representative proteins in tears from healthy controls and traumatic vegetative state patients. A List of 7 selected differentially-expressed proteins. Levels of CTSB (B), PRSS1 (C), S100A7 (D), GSTP1 (E), CFH (F), KNG1 (G), and ORM1 (H).
Article Snippet: ELISA and Receiver Operating Characteristic (ROC) Curve Analysis In the verification stage, the levels of 7 promising tear proteins [cystatin B (CTSB), protease, serine 1 (PRSS1), S100 calcium-binding protein A7 (S100A7), glutathione S-transferase P (GSTP1), complement factor H (CFH), kininogen 1 (KNG1), and alpha-1-acid glycoprotein 1 (ORM1)] were measured using the ELISA kits for human CSTB, human PRSS1, human CFH, human KNG1, and
Techniques: