Journal: Journal of Neuroscience Research

Article Title: Neuronal extracellular microRNAs miR‐124 and miR‐9 mediate cell–cell communication between neurons and microglia

doi: 10.1002/jnr.24344

Figure Lengend Snippet: Antibody characterization

Article Snippet: Rabbit polyclonal antibodies for human/mouse/rat HDLs (immunogen: KLH conjugated synthetic peptide from a 41–125 amino acid sequence of human HDL) were purchased from Biorbyt (cat#orb1003; 0.5 mg/ml, dilution 1:1,000; RRID: AB_2737031).

Techniques: Concentration Assay, Marker, Binding Assay, Immunofluorescence, Blocking Assay, Sequencing, Western Blot

Journal: Proceedings of SPIE--the International Society for Optical Engineering

Article Title: Fluorescence Image-Guided Surgery – a Perspective on Contrast Agent Development

doi: 10.1117/12.2545292

Figure Lengend Snippet: Novel FGS contrast agents under clinical development.

Article Snippet: RGD peptide -cy7 (ORL-1) 84 – 88 , Alpha(v) beta(3) integrin targeting peptide labeled with cy7. Topically applied to skin surface. , , , Cy7 , Orlucent , Melanoma , , NCT03535077 , recruiting.

Techniques: Single Photon Emission Computed Tomography, Fluorescence, Labeling, Injection, Activation Assay, Imaging, Positron Emission Tomography, IV Injection, Clinical Proteomics, Binding Assay, Ointment

Journal:

Article Title: Nonphotic entrainment of the circadian body temperature rhythm by the selective ORL1 receptor agonist W-212393 in rats

doi: 10.1038/sj.bjp.0706311

Figure Lengend Snippet: Binding affinity of W-212393 for ORL1, μ , δ , κ opioid receptors and serotonin transporter (SERT)

Article Snippet: W-212393 ( ) and an ORL1 antagonist J-113397 ( Ozaki et al ., 2000 ) were synthesized at Mitsubishi Pharma Corporation (Japan).

Techniques: Binding Assay, Recombinant

Journal:

Article Title: Nonphotic entrainment of the circadian body temperature rhythm by the selective ORL1 receptor agonist W-212393 in rats

doi: 10.1038/sj.bjp.0706311

Figure Lengend Snippet: Effects of N/OFQ and W-212393 on the spontaneous action potential firing of SCN neurons. (a) SCN slice placed on MED. (b) Representative trace of spontaneous firing activity recorded by the marked electrode (white square) in (a). Effect of N/OFQ (c) and W-212393 (d). N/OFQ and W-212393 were applied by bath perfusion for 10 min. Both N/OFQ and W-212393 significantly suppress the activity of spontaneously firing rat SCN neurons. The suppressive effects of N/OFQ and W-212393 did not recover after extensive washes for up to 40 min. (e) ORL1 receptor antagonist J-113397 (1 μM) prevented the reduction of the spontaneous firing rate by N/OFQ (100 nM) and W-212393 (100 nM). J-113397 was applied by bath perfusion 10 min before N/OFQ application. N/OFQ and W-212393 were applied by bath perfusion for 10 min with 20 min interval.

Article Snippet: W-212393 ( ) and an ORL1 antagonist J-113397 ( Ozaki et al ., 2000 ) were synthesized at Mitsubishi Pharma Corporation (Japan).

Techniques: Activity Assay